WDR36 (NM_139281) Human Tagged ORF Clone

CAT#: RC214584

WDR36 (Myc-DDK-tagged)-Human WD repeat domain 36 (WDR36)

Product Images

Circular map for RC214584

• 

  Western blot validation of overexpression lysate (Cat# LY408337) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214584 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified WDR36 protein (Cat# TP314584). The protein was produced from HEK293T cells transfected with WDR36 cDNA clone (Cat# RC214584) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WDR36
• Synonyms: GLC1G; TA-WDRP; TAWDRP; UTP21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214584 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTTGCACTGAGGGCAGTCTCCGGAAACGCGATTCGCAGCGGGCGCCGGAAGCGGTGTTGTGTCTGC
  AGCTCTGGCAGAGGACTGTTCCACTAGACACGCTGAAGGGACTGGGTACGTGTTTTCCTTCAGGACCAGA
  GCTGAGAGGAGCTGGGATCGCGGCGGCAATGGAACGGGCCTCAGAAAGGCGCACGGCCAGCGCGCTTTTT
  GCGGGGTTCCGGGCCTTGGGACTTTTCAGCAACGACATTCCACACGTGGTGCGGTTCAGCGCGCTCAAGC
  GCCGGTTCTATGTAACAACCTGCGTGGGCAAGAGTTTCCACACCTATGACGTTCAGAAACTTAGTCTGGT
  TGCAGTAAGTAATTCTGTTCCACAGGATATCTGCTGTATGGCAGCTGATGGCAGATTAGTCTTTGCTGCT
  TATGGAAATGTTTTCTCTGCATTTGCCCGTAATAAAGAGATAGTACATACCTTTAAGGGTCATAAGGCAG
  AAATCCATTTCTTGCAACCCTTTGGAGACCACATTATCTCTGTTGATACTGATGGCATTCTTATTATTTG
  GCACATATATTCAGAAGAAGAATACCTGCAGTTGACTTTTGATAAATCAGTATTTAAAATTTCTGCAATT
  TTGCATCCAAGTACCTACTTGAATAAAATACTTCTGGGCAGTGAACAAGGAAGCCTGCAGTTGTGGAATG
  TAAAATCCAATAAACTTCTATATACATTTCCAGGATGGAAAGTTGGAGTGACAGCTCTTCAGCAGGCACC
  AGCCGTGGATGTTGTTGCTATTGGTCTTATGTCAGGTCAAGTTATCATTCACAACATTAAATTTAATGAA
  ACATTAATGAAGTTTCGTCAAGACTGGGGACCCATTACTTCAATTTCATTTCGCACAGATGGTCATCCAG
  TAATGGCAGCTGGAAGCCCATGTGGCCATATTGGACTCTGGGATCTAGAAGACAAAAAATTAATCAACCA
  AATGAGAAATGCACACTCTACAGCAATTGCCGGACTGACATTTCTCCATAGAGAGCCACTTCTTGTCACA
  AATGGCGCTGACAATGCTCTTAGGATATGGATATTTGATGGTCCTACAGGTGAAGGCCGACTTTTGAGAT
  TCAGAATGGGTCATAGTGCTCCTCTTACCAATATCAGATATTATGGACAGAATGGACAGCAGATTCTAAG
  TGCAAGTCAAGATGGAACTCTTCAGTCATTTTCCACGGTACATGAAAAATTCAATAAGAGCTTGGGACAT
  GGATTAATAAATAAAAAGAGAGTTAAACGTAAAGGACTTCAGAATACCATGTCAGTGAGACTTCCACCCA
  TCACAAAGTTTGCAGCAGAGGAAGCTCGTGAAAGTGACTGGGATGGTATCATTGCTTGCCATCAAGGTAA
  GCTATCTTGCTCAACCTGGAATTATCAGAAATCTACAATAGGCGCTTACTTTCTCAAGCCAAAAGAGTTG
  AAGAAAGATGACATAACTGCAACAGCAGTGGATATAACTTCTTGTGGAAACTTTGCTGTAATTGGCCTCT
  CATCAGGAACTGTAGATGTATATAACATGCAGTCTGGCATACATCGAGGAAGTTTTGGCAAGGATCAAGC
  TCACAAGGGATCTGTTAGAGGTGTCGCAGTGGATGGATTAAACCAGTTGACAGTTACAACTGGTAGTGAA
  GGATTACTCAAATTCTGGAACTTTAAAAACAAAATTTTAATCCATTCTGTGAGCCTCAGTTCATCTCCAA
  ATATCATGTTGCTACATAGGGACAGTGGCATTCTGGGACTCGCCTTGGATGACTTCTCCATTAGTGTTCT
  GGACATAGAAACTAGGAAGATTGTCAGAGAGTTTTCTGGACACCAAGGCCAAATAAATGACATGGCTTTT
  AGTCCTGATGGTCGTTGGTTAATAAGTGCTGCGATGGATTGCTCTATTAGGACTTGGGACCTTCCTTCTG
  GGTGCCTTATAGACTGCTTTTTGTTGGACTCGGCTCCTCTCAATGTTTCTATGTCTCCTACTGGAGACTT
  TCTGGCAACTTCCCATGTGGACCACCTTGGAATTTATCTATGGTCCAATATTTCCCTGTATTCAGTTGTT
  TCATTACGGCCACTTCCTGCAGATTATGTCCCTTCAATAGTGATGCTTCCTGGTACTTGTCAAACCCAAG
  ATGTAGAAGTATCAGAAGAAACAGTAGAACCAAGTGATGAATTGATAGAATATGATTCGCCAGAACAGTT
  GAATGAGCAATTGGTGACTCTTTCACTTCTTCCTGAATCACGATGGAAAAACCTTCTTAACCTTGATGTT
  ATTAAGAAAAAGAATAAACCAAAGGAACCACCCAAAGTACCCAAATCAGCACCATTTTTCATTCCAACAA
  TTCCTGGCCTTGTACCCAGATATGCTGCACCTGAACAAAATAATGATCCCCAGCAGTCTAAAGTGGTAAA
  TCTTGGAGTTTTGGCTCAAAAATCAGATTTCTGCTTGAAACTTGAAGAAGGACTGGTAAATAATAAGTAT
  GACACTGCTCTCAACCTTCTGAAAGAATCAGGCCCATCAGGAATTGAAACAGAGCTGCGAAGCTTGTCTC
  CTGATTGTGGTGGGTCCATAGAAGTTATGCAGAGCTTCTTGAAAATGATTGGGATGATGCTGGACAGAAA
  GCGTGATTTTGAGTTAGCCCAGGCATACCTTGCATTGTTTCTAAAGTTACACCTTAAAATGCTTCCTTCA
  GAGCCAGTACTCCTAGAAGAAATAACAAATTTGTCATCCCAGGTGGAAGAAAACTGGACCCATTTGCAAT
  CACTCTTCAATCAAAGCATGTGTATTTTAAATTATCTCAAAAGTGCTTTGTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214584 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MCCTEGSLRKRDSQRAPEAVLCLQLWQRTVPLDTLKGLGTCFPSGPELRGAGIAAAMERASERRTASALF
  AGFRALGLFSNDIPHVVRFSALKRRFYVTTCVGKSFHTYDVQKLSLVAVSNSVPQDICCMAADGRLVFAA
  YGNVFSAFARNKEIVHTFKGHKAEIHFLQPFGDHIISVDTDGILIIWHIYSEEEYLQLTFDKSVFKISAI
  LHPSTYLNKILLGSEQGSLQLWNVKSNKLLYTFPGWKVGVTALQQAPAVDVVAIGLMSGQVIIHNIKFNE
  TLMKFRQDWGPITSISFRTDGHPVMAAGSPCGHIGLWDLEDKKLINQMRNAHSTAIAGLTFLHREPLLVT
  NGADNALRIWIFDGPTGEGRLLRFRMGHSAPLTNIRYYGQNGQQILSASQDGTLQSFSTVHEKFNKSLGH
  GLINKKRVKRKGLQNTMSVRLPPITKFAAEEARESDWDGIIACHQGKLSCSTWNYQKSTIGAYFLKPKEL
  KKDDITATAVDITSCGNFAVIGLSSGTVDVYNMQSGIHRGSFGKDQAHKGSVRGVAVDGLNQLTVTTGSE
  GLLKFWNFKNKILIHSVSLSSSPNIMLLHRDSGILGLALDDFSISVLDIETRKIVREFSGHQGQINDMAF
  SPDGRWLISAAMDCSIRTWDLPSGCLIDCFLLDSAPLNVSMSPTGDFLATSHVDHLGIYLWSNISLYSVV
  SLRPLPADYVPSIVMLPGTCQTQDVEVSEETVEPSDELIEYDSPEQLNEQLVTLSLLPESRWKNLLNLDV
  IKKKNKPKEPPKVPKSAPFFIPTIPGLVPRYAAPEQNNDPQQSKVVNLGVLAQKSDFCLKLEEGLVNNKY
  DTALNLLKESGPSGIETELRSLSPDCGGSIEVMQSFLKMIGMMLDRKRDFELAQAYLALFLKLHLKMLPS
  EPVLLEEITNLSSQVEENWTHLQSLFNQSMCILNYLKSALL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139281
• ORF Size: 2853 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139281.2, NP_644810.1
• RefSeq Size: 6611 bp
• RefSeq ORF: 2688 bp
• Locus ID: 134430
• UniProt ID: Q8NI36
• Cytogenetics: 5q22.1
• Domains: WD40, Utp21
• MW: 105.3 kDa
• Gene Summary: This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Mutations in this gene have been associated with adult-onset primary open-angle glaucoma (POAG). [provided by RefSeq, Jul 2008]