KIF1A (NM_004321) Human Tagged ORF Clone

CAT#: RC214527

KIF1A (Myc-DDK-tagged)-Human kinesin family member 1A (KIF1A)

Product Images

Circular map for RC214527

• 

  Western blot validation of overexpression lysate (Cat# LY418066) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214527 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KIF1A
• Synonyms: ATSV; C2orf20; HSN2C; MRD9; NESCAVS; SPG30; UNC104
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214527 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGGGCTTCGGTGAAGGTGGCGGTGCGGGTCCGCCCCTTCAATTCCCGGGAAATGAGCCGTGACT
  CCAAGTGCATCATTCAGATGTCTGGAAGCACCACCACCATTGTTAACCCCAAACAGCCCAAGGAGACGCC
  CAAAAGCTTCAGCTTTGACTACTCCTACTGGTCGCACACCTCACCTGAGGACATCAACTACGCGTCGCAG
  AAGCAGGTGTACCGGGACATCGGCGAGGAGATGCTGCAGCATGCCTTTGAGGGATACAACGTGTGCATCT
  TCGCCTATGGGCAGACGGGTGCCGGCAAGTCCTACACCATGATGGGCAAGCAGGAGAAGGACCAGCAGGG
  CATCATCCCACAGCTCTGCGAGGACCTCTTCTCTCGGATCAACGACACGACCAACGACAACATGTCCTAC
  TCCGTGGAGGTCAGCTACATGGAGATTTACTGTGAGCGCGTCCGTGACCTCCTGAACCCCAAGAACAAGG
  GCAACCTTCGCGTGAGGGAGCACCCACTGCTGGGGCCCTACGTGGAGGACCTCTCCAAGCTGGCTGTCAC
  CTCCTACAATGACATCCAGGACCTCATGGACTCAGGGAACAAGGCCAGGACCGTGGCGGCCACCAACATG
  AATGAGACCAGCAGTCGCTCCCACGCCGTCTTCAACATCATCTTCACCCAGAAGCGCCATGACGCAGAGA
  CCAATATCACCACGGAGAAGGTGAGCAAAATCAGCCTGGTGGACCTGGCTGGGAGCGAGCGGGCTGACTC
  CACGGGAGCCAAGGGCACGCGCCTCAAGGAGGGGGCCAACATCAACAAGTCGCTGACCACCCTGGGCAAG
  GTCATCTCCGCCCTGGCTGAAATGGACTCCGGACCCAACAAGAACAAGAAAAAGAAGAAGACAGATTTCA
  TTCCGTACCGAGATTCCGTGTTGACCTGGCTCCTCCGGGAAAACCTGGGCGGTAACTCAAGGACAGCTAT
  GGTGGCAGCCTTGAGTCCTGCAGACATCAACTACGATGAGACCCTTAGCACGCTGAGGTATGCTGACCGG
  GCCAAGCAGATCCGCTGCAATGCTGTCATCAATGAGGACCCCAACAACAAGCTGATCCGCGAGCTGAAGG
  ATGAGGTGACCCGGCTGCGGGACCTTCTGTACGCCCAGGGTCTTGGCGACATCACTGACATGACCAATGC
  CCTGGTGGGTATGAGCCCCTCATCCTCGCTCTCAGCCCTGTCCAGCCGCGCGGCCTCCGTGTCCAGCCTC
  CACGAGCGCATCTTGTTTGCCCCGGGCAGCGAGGAGGCCATTGAAAGACTGAAGGAAACAGAGAAGATCA
  TAGCTGAGCTCAATGAGACCTGGGAGGAGAAGCTGCGGCGGACAGAAGCCATCCGGATGGAGAGGGAAGC
  CCTGCTGGCCGAGATGGGTGTGGCCATGAGGGAGGATGGCGGCACCTTGGGCGTATTCTCTCCCAAAAAG
  ACACCACACCTCGTCAACCTGAACGAGGACCCGCTGATGTCTGAGTGCCTGCTCTACTACATCAAGGATG
  GGATCACCAGAGTGGGCAGGGAGGATGGCGAGAGGCGGCAGGACATTGTTCTGAGTGGGCACTTCATCAA
  GGAGGAGCACTGCGTCTTCCGGAGCGACTCCAGGGGAGGCAGCGAAGCTGTGGTGACCTTGGAGCCCTGT
  GAGGGGGCAGACACCTACGTCAATGGCAAGAAAGTCACAGAGCCCAGCATCCTGCGTTCAGGAAACCGCA
  TCATCATGGGTAAGAGCCATGTGTTCCGGTTCAACCACCCCGAGCAGGCCCGGCAGGAGCGTGAGCGCAC
  GCCTTGTGCGGAGACGCCAGCTGAGCCTGTGGACTGGGCCTTCGCCCAGCGTGAGCTGCTGGAGAAGCAG
  GGCATCGACATGAAGCAGGAGATGGAGCAGAGGCTCCAGGAACTGGAGGACCAGTACCGCCGCGAGCGGG
  AGGAGGCCACCTACCTGCTGGAGCAGCAGCGGCTGGACTATGAGAGCAAGCTGGAGGCTCTGCAGAAGCA
  GATGGACTCCAGGTACTACCCGGAGGTGAACGAGGAGGAGGAGGAGCCCGAGGATGAAGTCCAGTGGACA
  GAGCGGGAGTGTGAGCTGGCGCTCTGGGCCTTCCGGAAGTGGAAGTGGTACCAGTTCACGTCTCTGCGGG
  ACCTGCTGTGGGGCAACGCCATCTTCCTCAAGGAGGCCAATGCCATCAGCGTGGAGCTGAAAAAGAAGGT
  ACAATTCCAGTTTGTCCTCCTGACGGACACACTCTACTCCCCTCTGCCACCCGACCTGCTGCCCCCAGAG
  GCCGCCAAAGACCGAGAGACGCGGCCCTTCCCCCGCACCATTGTGGCCGTGGAGGTCCAGGACCAGAAGA
  ACGGGGCCACCCACTACTGGACGCTGGAGAAGCTCAGGCAGCGTCTGGACCTGATGCGGGAGATGTACGA
  CCGCGCTGCAGAGGTGCCCTCCAGTGTCATCGAGGACTGTGACAACGTGGTGACCGGCGGAGACCCCTTC
  TATGACCGCTTCCCCTGGTTCCGGCTGGTGGGCAGGGCCTTCGTGTACCTGAGCAACCTGCTGTACCCCG
  TTCCCCTGGTACACCGTGTGGCAATCGTCAGCGAGAAGGGCGAGGTGAAGGGCTTCCTCCGCGTGGCCGT
  CCAGGCCATCTCAGCCGATGAAGAGGCCCCTGATTATGGCTCTGGCGTCCGCCAGTCGGGAACTGCTAAA
  ATCTCCTTTGATGACCAGCATTTTGAAAAGTTCCAGTCCGAGTCTTGCCCCGTGGTGGGGATGTCCCGCT
  CGGGAACCTCCCAGGAAGAGCTTCGCATCGTGGAGGGCCAGGGCCAGGGTGCAGACGTGGGGCCCTCAGC
  CGATGAAGTCAACAACAACACCTGTTCAGCAGTGCCCCCAGAAGGCCTCCTCCTAGACAGCTCTGAGAAA
  GCCGCCCTGGATGGGCCCCTGGATGCTGCCCTGGACCACCTCCGCCTGGGCAACACCTTCACCTTCCGTG
  TGACAGTCCTGCAGGCGTCCAGCATCTCTGCCGAATATGCCGACATCTTCTGCCAGTTCAACTTCATCCA
  CCGCCACGACGAGGCCTTCTCCACAGAGCCCCTGAAGAACACAGGCAGAGGCCCCCCACTTGGCTTCTAC
  CACGTCCAGAACATCGCAGTGGAGGTGACCAAGTCCTTCATTGAGTACATCAAGAGCCAGCCCATTGTTT
  TCGAGGTCTTTGGCCACTACCAGCAGCACCCGTTCCCGCCCCTCTGCAAGGACGTGCTCAGCCCCCTGAG
  GCCCTCGCGCCGCCACTTCCCTCGGGTCATGCCACTGTCCAAGCCAGTGCCCGCCACCAAGCTCAGCACA
  CTGACGCGGCCCTGTCCGGGACCCTGCCACTGCAAGTACGACCTGCTGGTCTACTTCGAGATCTGTGAGC
  TGGAGGCCAACGGCGATTACATCCCGGCCGTGGTGGACCACCGTGGGGGCATGCCATGCATGGGGACCTT
  CCTCCTCCACCAGGGCATCCAGCGACGGATTACGGTGACACTACTGCATGAGACAGGCAGCCATATCCGC
  TGGAAGGAAGTGCGCGAGCTGGTCGTGGGCCGCATCCGAAACACTCCAGAGACCGACGAGTCCCTGATCG
  ACCCCAACATCTTGTCTCTCAACATCCTCTCTTCCGGATACATCCACCCAGCCCAAGATGACCGGACCTT
  TTACCAATTTGAGGCTGCGTGGGACAGCTCCATGCACAACTCTCTCCTGCTGAACCGGGTCACCCCTTAT
  CGAGAGAAAATCTACATGACACTCTCCGCTTATATCGAGATGGAGAACTGCACCCAGCCGGCTGTTGTCA
  CCAAGGACTTCTGCATGGTCTTCTATTCCCGTGATGCCAAGCTGCCAGCCTCGCGCTCCATCCGCAACCT
  CTTTGGCAGTGGGAGCCTTCGGGCCTCAGAGAGTAACCGTGTGACTGGTGTGTACGAGCTCAGCCTGTGC
  CACGTGGCTGACGCGGGCAGCCCAGGGATGCAGCGCCGGCGCCGACGAGTCCTGGACACATCTGTGGCCT
  ATGTCCGGGGCGAGGAGAACCTGGCAGGCTGGAGGCCCCGGAGTGACAGTCTCATTCTGGACCACCAGTG
  GGAGCTGGAGAAGCTGAGCCTCCTGCAGGAGGTGGAGAAGACTAGGCACTACCTGCTCCTGCGGGAGAAG
  CTGGAGACCGCCCAGCGGCCTGTCCCGGAGGCACTGTCCCCGGCCTTCAGCGAGGACTCTGAGTCCCATG
  GCTCCTCCAGCGCCTCCTCCCCGCTCTCGGCTGAGGGCCGCCCATCACCCCTGGAGGCTCCCAACGAGAG
  GCAGCGGGAGCTGGCCGTCAAGTGCTTGCGCCTGCTCACGCACACATTCAACAGAGAGTACACACACAGC
  CACGTCTGCGTCAGTGCCAGCGAGAGCAAGCTCTCCGAGATGTCTGTCACCCTGCTCCGGGACCCGTCGA
  TGTCCCCTCTAGGGGTGGCCACTCTCACCCCCTCCTCCACTTGCCCCTCTCTGGTTGAAGGGCGGTACGG
  TGCCACTGACCTGAGGACCCCGCAGCCCTGCTCCCGGCCAGCCAGCCCAGAGCCCGAGCTGCTGCCAGAG
  GCCGACTCCAAGAAGCTCCCTTCCCCTGCCCGGGCAACAGAGACAGACAAGGAGCCCCAGCGCCTGCTGG
  TCCCTGACATCCAGGAGATCCGAGTCAGCCCGATCGTTTCCAAGAAGGGGTACCTGCACTTCCTGGAGCC
  GCACACGTCAGGCTGGGCCAGGCGCTTCGTGGTGGTGCGGCGCCCCTATGCCTACATGTACAACAGCGAC
  AAGGACACCGTGGAGCGGTTCGTGCTCAACCTGGCCACTGCCCAGGTGGAGTACAGTGAGGACCAGCAGG
  CTATGCTCAAGACACCCAACACATTCGCGGTGTGCACGGAACACCGCGGCATCCTGCTGCAGGCCGCCAG
  CGACAAGGACATGCATGACTGGCTGTACGCCTTCAACCCCCTCCTGGCCGGGACCATACGGTCCAAGCTC
  TCCAGAAGGAGGTCTGCCCAGATGCGGGTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214527 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQ
  KQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSY
  SVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNM
  NETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGK
  VISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADR
  AKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTNALVGMSPSSSLSALSSRAASVSSL
  HERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKK
  TPHLVNLNEDPLMSECLLYYIKDGITRVGREDGERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPC
  EGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQ
  GIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWT
  ERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPE
  AAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPF
  YDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAK
  ISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADVGPSADEVNNNTCSAVPPEGLLLDSSEK
  AALDGPLDAALDHLRLGNTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFY
  HVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLST
  LTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIR
  WKEVRELVVGRIRNTPETDESLIDPNILSLNILSSGYIHPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPY
  REKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLC
  HVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREK
  LETAQRPVPEALSPAFSEDSESHGSSSASSPLSAEGRPSPLEAPNERQRELAVKCLRLLTHTFNREYTHS
  HVCVSASESKLSEMSVTLLRDPSMSPLGVATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPELLPE
  ADSKKLPSPARATETDKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTSGWARRFVVVRRPYAYMYNSD
  KDTVERFVLNLATAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQAASDKDMHDWLYAFNPLLAGTIRSKL
  SRRRSAQMRV
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004321
• ORF Size: 5070 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004321.8
• RefSeq Size: 8743 bp
• RefSeq ORF: 5073 bp
• Locus ID: 547
• UniProt ID: Q12756
• Cytogenetics: 2q37.3
• Domains: FHA, kinesin, PH
• Protein Families: Druggable Genome
• MW: 191.1 kDa
• Gene Summary: The protein encoded by this gene is a member of the kinesin family and functions as an anterograde motor protein that transports membranous organelles along axonal microtubules. Mutations at this locus have been associated with spastic paraplegia-30 and hereditary sensory neuropathy IIC. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Apr 2012]