CNTN4 (NM_175612) Human Tagged ORF Clone

CAT#: RC214500

CNTN4 (Myc-DDK-tagged)-Human contactin 4 (CNTN4), transcript variant 2

Product Images

Circular map for RC214500

• 

  Western blot validation of overexpression lysate (Cat# LY403573) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214500 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CNTN4
• Synonyms: AXCAM; axonal-associated cell adhesion molecule; axonal cell adhesion molecule; BIG-2; CNTN4A; contactin 4; MGC33615; neural cell adhesion protein BIG-2; OTTHUMP00000147566; OTTHUMP00000147567
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214500 representing NM_175612
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCTGGATGCTGACAGTGCTGGCCTCAGCTGATGCCTCTAGATACGTGTTCAGGAATGAGAGCGTGC
  ACCCCTTCTCTCCCTTTGAGGTTAAAGTAGGTGTCTTCAACAACAAAGGAGAAGGCCCTTTCAGTCCCAC
  CACGGTGGTGTATTCTGCAGAAGAAGAACCCACCAAACCACCAGCCAGTATCTTTGCCAGAAGTCTTTCT
  GCCACAGATATTGAAGTTTTCTGGGCCTCCCCACTGGAGAAGAATAGAGGACGAATACAAGGTTATGAGG
  TTAAATATTGGAGACATGAAGACAAAGAAGAAAATGCTAGAAAAATACGAACAGTTGGAAATCAGACATC
  AACAAAAATCACGAACTTAAAAGGCAGTGTGCTGTATCACTTAGCTGTCAAGGCATATAATTCTGCTGGG
  ACAGGCCCCTCTAGTGCAACAGTCAATGTGACAACCCGAAAGCCACCACCAAGTCAACCCCCCGGAAACA
  TCATATGGAATTCATCAGACTCCAAAATTATCCTGAATTGGGATCAAGTGAAGGCCCTGGATAATGAGTC
  GGAAGTAAAAGGATACAAAGTCTTGTACAGATGGAACAGACAAAGCAGCACATCTGTCATTGAAACAAAT
  AAAACATCGGTGGAGCTTTCTTTGCCTTTCGATGAAGATTATATAATAGAAATTAAGCCATTCAGCGACG
  GAGGAGATGGCAGCAGCAGTGAACAAATTCGAATTCCAAAGATATCAAATGCCTACGCGAGAGGATCTGG
  GGCTTCCACTTCGAATGCATGTACGCTGTCAGCCATCAGTACAATAATGATTTCCCTCACAGCTAGGTCC
  AGTTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214500 representing NM_175612
  Red=Cloning site Green=Tags(s)
  
  MIWMLTVLASADASRYVFRNESVHPFSPFEVKVGVFNNKGEGPFSPTTVVYSAEEEPTKPPASIFARSLS
  ATDIEVFWASPLEKNRGRIQGYEVKYWRHEDKEENARKIRTVGNQTSTKITNLKGSVLYHLAVKAYNSAG
  TGPSSATVNVTTRKPPPSQPPGNIIWNSSDSKIILNWDQVKALDNESEVKGYKVLYRWNRQSSTSVIETN
  KTSVELSLPFDEDYIIEIKPFSDGGDGSSSEQIRIPKISNAYARGSGASTSNACTLSAISTIMISLTARS
  SL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175612
• ORF Size: 846 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175612.1, NP_783301.1
• RefSeq Size: 3072 bp
• RefSeq ORF: 848 bp
• Locus ID: 152330
• Cytogenetics: 3p26.3-p26.2
• Protein Families: Secreted Protein
• MW: 29.6 kDa
• Gene Summary: This gene encodes a member of the contactin family of immunoglobulins. Contactins are axon-associated cell adhesion molecules that function in neuronal network formation and plasticity. The encoded protein is a glycosylphosphatidylinositol-anchored neuronal membrane protein that may play a role in the formation of axon connections in the developing nervous system. Deletion or mutation of this gene may play a role in 3p deletion syndrome and autism spectrum disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2011]