FAM82A1 (RMDN2) (NM_144713) Human Tagged ORF Clone

CAT#: RC214471

RMDN2 (Myc-DDK-tagged)-Human family with sequence similarity 82, member A1 (FAM82A1), transcript variant 1

Product Images

Circular map for RC214471

• 

  Western blot validation of overexpression lysate (Cat# LY408164) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214471 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FAM82A1
• Synonyms: BLOCK18; FAM82A; FAM82A1; PRO34163; PYST9371; RMD-2; RMD2; RMD4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214471 representing NM_144713
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAAGTGCTTAAGCTGCTGCAAAGAGGACCAGAGCTTCCAACGATGTTCTCCAGAAGATCAAGTTA
  GTACAGACGCCCAGCATCGTGGAGCCTCTTCTATTTCACAACCAAGTATATCTCTTGGTCATAAAACATC
  TTATTCCCCTGTAACACATAAAGTCAATGCAGCCAAAGCAAGTAGAAGGTTATTATCTGTATCAAGTCCA
  TCTTTCTCTGAAAGAAGATATTCTTTATTTGTTGGATTTCAAAAAAGAAATGCTTCCCCTTACTGGCAAC
  AAAGTAGAGCTAACTTTGATTCTGAAGAAGACACAGGCTTCACTGATATAAAATCTTCCTCAGACCATTG
  TGGTAGCTTTATTTCCCGCAGAAGACGTTTCTCATCCCGTAAACTAAGTATAGTTTCCTATTACAAGAGT
  GCCATTTTTTTTGATCCTCAAGCAAGTGGCCAGAATGTGTTTAATCTAAATGAAATCGAAATCTTTTCTA
  AAACTTCAAGTAATACTGATGCTAAAAAACATATAACCATCTCTGCTCCTGAATATAACACTAAAAATTT
  TAAGAATTTTGAAACAAACACTACTTCTCCAGCCTTTGGGAACACTATTGATACAGCCTCCTATCAACAA
  AGCACATCATCCTTCTTTTCTCTTGCAAGTGATATTTCCTCTCCCGATCAACAAAATGGAATTGCCAATG
  ATATTCAACAAAGGGGCCAATTATGTAAAGATTTAAAAGATTTCCTTCATCCTCGTCCTGAAAGTTACAG
  CACAGGTCATTCTCCAATCATGATTCCACAGCATCCCTCTCAAAGTGGAACTTTCCCATTCCTCCATAAA
  GCTGGATTTTCTTCATCTTATAAAAATTCTGGTTGCTTTATCCCACCTCAAAGCGAATTAACTTCAGGCC
  TGTTTGAAGATGAAGACTTTGCTGTCTTGTTTCAAGATGAAGACAGATCTTCACCTATTGAAATTCCTAA
  AATAAGGTATATTACAGCTAATACTGACACAGAAGAACAGAGTTTTCCAGTCCCTAAGGCATTTAACACA
  CGTGTAGAGGAATTAAATTTAGATGTCCTTCTTCAGAAGGTAGATCATTTACGTATGAGTGAGTCTGGCA
  AGTCGGAGAGTTTTGAACTACTTCGTGACCACAAAGAAAAGTTTAGAGATGAAATAGAGTTTATGTGGCG
  ATTTGCTCGTGCTTATGGAGACATGTATGAACTATCTACAAACACACAAGAAAAGAAACATTATGCTAAT
  ATTGGAAAAACTTTAAGTGAAAGAGCTATTAATAGAGCACCCATGAATGGACATTGTCATCTGTGGTATG
  CAGTTTTGTGTGGCTATGTATCTGAGTTTGAGGGTTTACAAAACAAAATCAACTATGGGCACCTCTTCAA
  GGAACATCTAGATATAGCAATCAAACTTTTACCAGAGGAACCCTTTCTATATTACCTCAAAGGGAGATAC
  TGCTATACTGTCTCAAAACTGAGCTGGATTGAGAAAAAAATGGCTGCTACTCTGTTTGGAAAAATACCAT
  CTTCAACTGTACAAGAAGCTTTACACAATTTCCTTAAGGCTGAAGAACTATGCCCTGGTTATTCTAATCC
  CAATTACATGTACTTAGCAAAGTGTTATACTGATCTTGAGGAAAACCAGAATGCTTTGAAGTTCTGTAAT
  TTGGCTTTATTGCTTCCTACTGTTACCAAAGAGGAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214471 representing NM_144713
  Red=Cloning site Green=Tags(s)
  
  MGKCLSCCKEDQSFQRCSPEDQVSTDAQHRGASSISQPSISLGHKTSYSPVTHKVNAAKASRRLLSVSSP
  SFSERRYSLFVGFQKRNASPYWQQSRANFDSEEDTGFTDIKSSSDHCGSFISRRRRFSSRKLSIVSYYKS
  AIFFDPQASGQNVFNLNEIEIFSKTSSNTDAKKHITISAPEYNTKNFKNFETNTTSPAFGNTIDTASYQQ
  STSSFFSLASDISSPDQQNGIANDIQQRGQLCKDLKDFLHPRPESYSTGHSPIMIPQHPSQSGTFPFLHK
  AGFSSSYKNSGCFIPPQSELTSGLFEDEDFAVLFQDEDRSSPIEIPKIRYITANTDTEEQSFPVPKAFNT
  RVEELNLDVLLQKVDHLRMSESGKSESFELLRDHKEKFRDEIEFMWRFARAYGDMYELSTNTQEKKHYAN
  IGKTLSERAINRAPMNGHCHLWYAVLCGYVSEFEGLQNKINYGHLFKEHLDIAIKLLPEEPFLYYLKGRY
  CYTVSKLSWIEKKMAATLFGKIPSSTVQEALHNFLKAEELCPGYSNPNYMYLAKCYTDLEENQNALKFCN
  LALLLPTVTKEEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144713
• ORF Size: 1719 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144713.3
• RefSeq Size: 1986 bp
• RefSeq ORF: 1722 bp
• Locus ID: 151393
• UniProt ID: Q96LZ7
• Cytogenetics: 2p22.2
• MW: 65 kDa