FOG2 (ZFPM2) (NM_012082) Human Tagged ORF Clone

CAT#: RC214338

ZFPM2 (Myc-DDK-tagged)-Human zinc finger protein, multitype 2 (ZFPM2)

Product Images

Circular map for RC214338

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FOG2
• Synonyms: DIH3; FOG2; hFOG-2; SRXY9; ZC2HC11B; ZNF89B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214338 representing NM_012082
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCGGCGAAAGCAAAGCAAACCCCGGCAGATCAAACGGCCGCTTGAAGATGCCATTGAAGATGAGG
  AAGAAGAATGTCCATCAGAGGAAACAGACATCATCTCCAAAGGAGACTTTCCATTGGAGGAAAGCTTTTC
  CACAGAATTTGGGCCTGAAAATCTGAGCTGCGAAGAAGTGGAATACTTTTGTAACAAAGGTGATGATGAA
  GGAATCCAGGAGACAGCAGAATCAGATGGGGACACACAGTCAGAGAAACCGGGGCAACCTGGAGTTGAGA
  CAGACGACTGGGATGGACCAGGAGAGCTGGAGGTGTTTCAGAAAGATGGGGAACGAAAAATTCAGAGTCG
  ACAGCAACTTCCAGTGGGAACAACCTGGGGGCCGTTTCCTGGGAAGATGGACTTGAATAATAATTCTTTG
  AAGACAAAGGCTCAGGTCCCAATGGTGCTGACTGCTGGTCCCAAGTGGTTGCTGGATGTGACTTGGCAAG
  GAGTGGAAGACAACAAAAACAACTGCATTGTGTACAGCAAAGGGGGTCAGCTTTGGTGTACAACTACGAA
  GGCCATCTCTGAGGGTGAAGAGCTAATTGCCTTTGTGGTGGATTTTGACTCAAGGCTACAAGCTGCCAGT
  CAGATGACTCTCACAGAAGGGATGTACCCTGCACGCCTGCTGGACTCAATTCAGCTGCTTCCTCAGCAAG
  CTGCCATGGCTTCTATTTTGCCCACAGCTATTGTCAATAAGGATATATTCCCTTGCAAGTCCTGTGGCAT
  CTGGTATCGGAGTGAGCGGAATCTGCAGGCCCATTTGATGTACTACTGCAGTGGGAGGCAAAGAGAAGCT
  GCTCCGGTGTCAGAGGAAAATGAAGACAGTGCCCATCAGATTTCCAGCCTGTGCCCCTTCCCACAGTGCA
  CCAAGAGCTTTTCAAATGCTCGAGCTCTAGAAATGCACCTGAATTCACACAGTGGAGTGAAAATGGAAGA
  ATTCCTGCCCCCTGGTGCTAGTCTAAAATGCACCGTCTGTAGCTACACTGCTGATTCCGTGATCAACTTT
  CACCAACACCTGTTCTCCCATCTCACTCAAGCTGCCTTCCGATGTAATCACTGCCATTTCGGCTTCCAGA
  CTCAGAGGGAGTTATTGCAGCACCAGGAGCTCCATGTCCCTAGCGGCAAACTTCCCAGAGAAAGTGACAT
  GGAACACTCTCCAAGTGCAACTGAAGACAGCTTACAGCCAGCCACAGACTTATTGACCAGAAGCGAACTT
  CCCCAGAGCCAAAAGGCCATGCAGACTAAAGATGCGAGCTCTGACACAGAGCTGGACAAGTGTGAGAAAA
  AGACTCAGCTCTTTCTCACGAACCAGAGACCAGAGATACAGCCTACAACAAATAAACAAAGCTTTTCTTA
  CACAAAAATAAAGTCTGAGCCCTCTAGCCCAAGACTTGCCTCATCTCCAGTTCAGCCTAATATTGGGCCT
  TCTTTCCCTGTGGGCCCTTTCCTATCTCAGTTTTCTTTCCCCCAAGATATCACCATGGTCCCTCAAGCTT
  CAGAGATCTTAGCTAAGATGTCTGAACTGGTGCATCGGCGACTGAGGCATGGCAGTAGTAGCTACCCTCC
  CGTCATTTACAGCCCTTTGATGCCCAAGGGGGCTACTTGTTTTGAGTGTAACATAACATTCAATAATTTG
  GATAATTATCTAGTGCACAAAAAGCATTATTGCAGCAGCCGATGGCAGCAGATGGCTAAGTCCCCAGAGT
  TCCCTAGTGTGTCAGAAAAGATGCCTGAAGCTTTGAGTCCCAACACTGGCCAAACCTCCATAAACCTTCT
  CAACCCAGCTGCTCATTCTGCTGATCCTGAGAATCCACTTCTTCAAACATCTTGCATCAATTCTTCCACT
  GTCTTAGATTTAATTGGGCCAAATGGGAAGGGCCATGACAAGGACTTTTCCACTCAAACTAAGAAGCTCT
  CCACCTCCAGTAACAATGATGACAAAATTAATGGAAAACCTGTTGATGTGAAAAATCCCAGTGTCCCCTT
  AGTGGATGGGGAAAGTGACCCAAATAAGACTACCTGTGAAGCTTGCAACATTACCTTCAGCCGGCACGAA
  ACATACATGGTCCACAAACAGTATTACTGTGCTACACGCCACGACCCTCCACTGAAGAGGTCTGCTTCCA
  ACAAAGTGCCTGCCATGCAGAGAACCATGCGCACACGCAAGCGCAGAAAGATGTATGAGATGTGCCTACC
  TGAGCAGGAACAAAGGCCTCCACTGGTTCAGCAGAGATTTCTTGACGTAGCCAACCTCAATAATCCTTGT
  ACCTCCACTCAAGAACCCACAGAAGGGCTAGGAGAGTGCTACCACCCAAGATGTGATATCTTTCCAGGAA
  TTGTCTCTAAACACTTGGAAACTTCTCTGACGATCAACAAGTGTGTTCCAGTTTCCAAATGTGATACTAC
  TCATTCCAGTGTTTCCTGCCTAGAGATGGACGTGCCCATAGATCTCAGCAAAAAGTGTTTATCTCAGTCT
  GAGCGGACGACCACGTCTCCCAAAAGGCTGCTGGACTATCACGAGTGCACTGTGTGCAAGATCAGTTTCA
  ATAAGGTAGAAAACTATCTGGCCCACAAGCAGAATTTCTGCCCGGTTACTGCACATCAGCGTAATGACCT
  GGGTCAACTGGACGGCAAAGTGTTTCCGAATCCAGAAAGCGAACGAAACAGCCCTGATGTCAGCTACGAA
  AGAAGCATAATAAAATGTGAGAAAAATGGGAATTTGAAGCAGCCTTCCCCCAATGGAAACTTATTTTCAT
  CCCACCTAGCAACCCTGCAAGGCTTGAAGGTCTTTAGTGAAGCTGCTCAGCTCATTGCTACAAAAGAAGA
  AAACAGACATTTGTTTCTTCCACAATGCCTTTACCCTGGAGCAATAAAGAAAGCAAAAGGAGCCGACCAG
  CTTTCTCCATATTATGGAATCAAGCCAAGTGATTATATTTCTGGTTCTCTTGTCATCCATAACACTGACA
  TCGAGCAAAGCAGAAATGCAGAAAATGAATCTCCTAAAGGCCAGGCTTCCTCAAATGGGTGTGCTGCGCT
  GAAGAAAGATTCTCTGCCATTGTTGCCCAAAAATCGAGGAATGGTAATAGTGAATGGTGGACTGAAACAA
  GATGAGAGACCTGCTGCCAACCCACAGCAAGAGAACATTTCCCAGAATCCTCAGCACGAAGACGACCACA
  AATCTCCCTCGTGGATCTCTGAGAACCCATTAGCTGCCAATGAGAATGTCTCACCAGGAATTCCCTCAGC
  AGAGGAACAGTTGTCTAGTATAGCAAAAGGTGTGAATGGTTCCAGCCAGGCTCCAACCAGTGGGAAATAT
  TGCCGGCTATGTGATATCCAGTTCAACAACCTTTCAAACTTTATAACTCACAAGAAGTTTTATTGCTCAT
  CACATGCAGCAGAACATGTCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214338 representing NM_012082
  Red=Cloning site Green=Tags(s)
  
  MSRRKQSKPRQIKRPLEDAIEDEEEECPSEETDIISKGDFPLEESFSTEFGPENLSCEEVEYFCNKGDDE
  GIQETAESDGDTQSEKPGQPGVETDDWDGPGELEVFQKDGERKIQSRQQLPVGTTWGPFPGKMDLNNNSL
  KTKAQVPMVLTAGPKWLLDVTWQGVEDNKNNCIVYSKGGQLWCTTTKAISEGEELIAFVVDFDSRLQAAS
  QMTLTEGMYPARLLDSIQLLPQQAAMASILPTAIVNKDIFPCKSCGIWYRSERNLQAHLMYYCSGRQREA
  APVSEENEDSAHQISSLCPFPQCTKSFSNARALEMHLNSHSGVKMEEFLPPGASLKCTVCSYTADSVINF
  HQHLFSHLTQAAFRCNHCHFGFQTQRELLQHQELHVPSGKLPRESDMEHSPSATEDSLQPATDLLTRSEL
  PQSQKAMQTKDASSDTELDKCEKKTQLFLTNQRPEIQPTTNKQSFSYTKIKSEPSSPRLASSPVQPNIGP
  SFPVGPFLSQFSFPQDITMVPQASEILAKMSELVHRRLRHGSSSYPPVIYSPLMPKGATCFECNITFNNL
  DNYLVHKKHYCSSRWQQMAKSPEFPSVSEKMPEALSPNTGQTSINLLNPAAHSADPENPLLQTSCINSST
  VLDLIGPNGKGHDKDFSTQTKKLSTSSNNDDKINGKPVDVKNPSVPLVDGESDPNKTTCEACNITFSRHE
  TYMVHKQYYCATRHDPPLKRSASNKVPAMQRTMRTRKRRKMYEMCLPEQEQRPPLVQQRFLDVANLNNPC
  TSTQEPTEGLGECYHPRCDIFPGIVSKHLETSLTINKCVPVSKCDTTHSSVSCLEMDVPIDLSKKCLSQS
  ERTTTSPKRLLDYHECTVCKISFNKVENYLAHKQNFCPVTAHQRNDLGQLDGKVFPNPESERNSPDVSYE
  RSIIKCEKNGNLKQPSPNGNLFSSHLATLQGLKVFSEAAQLIATKEENRHLFLPQCLYPGAIKKAKGADQ
  LSPYYGIKPSDYISGSLVIHNTDIEQSRNAENESPKGQASSNGCAALKKDSLPLLPKNRGMVIVNGGLKQ
  DERPAANPQQENISQNPQHEDDHKSPSWISENPLAANENVSPGIPSAEEQLSSIAKGVNGSSQAPTSGKY
  CRLCDIQFNNLSNFITHKKFYCSSHAAEHVK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012082
• ORF Size: 3453 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012082.4
• RefSeq Size: 4507 bp
• RefSeq ORF: 3456 bp
• Locus ID: 23414
• UniProt ID: Q8WW38
• Cytogenetics: 8q23.1
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• MW: 128 kDa
• Gene Summary: The zinc finger protein encoded by this gene is a widely expressed member of the FOG family of transcription factors. The family members modulate the activity of GATA family proteins, which are important regulators of hematopoiesis and cardiogenesis in mammals. It has been demonstrated that the protein can both activate and down-regulate expression of GATA-target genes, suggesting different modulation in different promoter contexts. A related mRNA suggests an alternatively spliced product but this information is not yet fully supported by the sequence. [provided by RefSeq, Jul 2008]