ADA2 (NM_177405) Human Tagged ORF Clone

CAT#: RC214292

CECR1 (Myc-DDK-tagged)-Human cat eye syndrome chromosome region, candidate 1 (CECR1), transcript variant 2

Product Images

Circular map for RC214292

• 

  Western blot validation of overexpression lysate (Cat# LY406155) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214292 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADA2
• Synonyms: ADGF; CECR1; IDGFL; PAN; SNEDS; VAIHS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214292 representing NM_177405
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTCCCTGGAATGGAACTGGGCTCTGGTGTATGAGCTCAGTGGAGAGCACCATGACGAAGAGTGGT
  CAGTGAAGACTTACCAGGAAGTAGCTCAGAAGTTTGTGGAAACTCACCCTGAGTTTATTGGAATCAAAAT
  CATTTATTCGGATCACAGATCCAAAGATGTGGCTGTCATCGCAGAATCCATCCGAATGGCCATGGGGCTC
  CGAATCAAGTTCCCCACGGTGGTGGCAGGGTTTGACCTGGTGGGGCATGAGGACACTGGCCACTCCTTGC
  ATGACTACAAGGAAGCTCTGATGATCCCCGCCAAGGATGGCGTTAAGCTGCCTTACTTCTTCCACGCCGG
  AGAAACAGACTGGCAGGGTACTTCCATAGACAGGAACATTCTGGATGCTCTGATGCTGAACACTACCAGA
  ATCGGCCATGGATTTGCTTTGAGCAAACACCCCGCAGTCAGGACTTACTCCTGGAAAAAGGACATCCCCA
  TAGAAGTCTGTCCCATCTCTAACCAGGTGCTGAAACTGGTGTCTGACTTGAGGAACCACCCTGTAGCCAC
  TCTGATGGCCACTGGGCACCCCATGGTGATCAGCTCTGATGACCCAGCTATGTTTGGTGCCAAAGGCTTG
  TCCTATGATTTCTATGAGGTCTTCATGGGCATTGGGGGGATGAAGGCTGACCTGAGGACCCTCAAACAGC
  TGGCCATGAACTCTATCAAGTACAGTACCCTGTTGGAGAGTGAGAAAAATACTTTCATGGAAATCTGGAA
  GAAGAGATGGGATAAGTTCATAGCAGATGTGGCTACAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214292 representing NM_177405
  Red=Cloning site Green=Tags(s)
  
  MDSLEWNWALVYELSGEHHDEEWSVKTYQEVAQKFVETHPEFIGIKIIYSDHRSKDVAVIAESIRMAMGL
  RIKFPTVVAGFDLVGHEDTGHSLHDYKEALMIPAKDGVKLPYFFHAGETDWQGTSIDRNILDALMLNTTR
  IGHGFALSKHPAVRTYSWKKDIPIEVCPISNQVLKLVSDLRNHPVATLMATGHPMVISSDDPAMFGAKGL
  SYDFYEVFMGIGGMKADLRTLKQLAMNSIKYSTLLESEKNTFMEIWKKRWDKFIADVATK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177405
• ORF Size: 810 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177405.3
• RefSeq Size: 3071 bp
• RefSeq ORF: 813 bp
• Locus ID: 51816
• UniProt ID: Q9NZK5
• Cytogenetics: 22q11.1
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• MW: 30.5 kDa
• Gene Summary: This gene encodes a member of a subfamily of the adenosine deaminase protein family. The encoded protein is one of two adenosine deaminases found in humans, which regulate levels of the signaling molecule, adenosine. The encoded protein is secreted from monocytes undergoing differentiation and may regulate cell proliferation and differentiation. This gene may be responsible for some of the phenotypic features associated with cat eye syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]