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TPR (NM_003292) Human Tagged ORF Clone
CAT#: RC214233
- TrueORF®
TPR (Myc-DDK-tagged)-Human translocated promoter region (to activated MET oncogene) (TPR). Note: ORF is codon optimized
ORF Plasmid: tGFP
"NM_003292" in other vectors (2)
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | TPR |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214233 ORF sequence, codon optimized.
Due to the complexity of NM_003292, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGGTCCTCCAGCAAGTGCTGGAGAGAACTGAACTGAATAAATTGCCCAAGTCCGTGCAGAATA AGCTTGAAAAGTTCCTTGCAGATCAACAATCTGAGATCGACGGGTTGAAGGGTAGACACGAAAAGTTTAA GGTTGAGTCAGAACAACAATACTTCGAGATCGAGAAAAGGCTGTCTCATTCTCAAGAGCGCTTGGTGAAC GAGACACGCGAGTGTCAGTCCCTTAGACTTGAACTGGAGAAGCTCAATAACCAGTTGAAGGCACTGACAG AAAAGAACAAAGAGCTGGAGATCGCACAAGACAGAAATATAGCCATTCAATCCCAGTTTACCAGAACAAA GGAAGAGCTGGAAGCCGAGAAAAGAGACTTGATCCGCACTAACGAACGCTTGAGCCAGGAACTGGAGTAT CTGACTGAGGATGTTAAAAGGCTGAACGAAAAGCTGAAGGAAAGCAATACGACGAAGGGAGAGCTGCAGC TCAAATTGGACGAACTTCAGGCATCCGATGTGAGTGTCAAGTATAGGGAGAAACGCCTGGAGCAGGAGAA GGAGTTGCTGCACTCCCAGAACACCTGGCTCAACACAGAGCTGAAAACAAAAACTGACGAGCTTCTCGCG TTGGGTAGGGAAAAAGGCAACGAGATCCTTGAGCTCAAGTGTAACCTCGAGAATAAGAAGGAGGAGGTAT CCCGCCTGGAGGAGCAGATGAACGGACTGAAAACTAGCAACGAACACTTGCAGAAACACGTCGAGGATCT GCTCACCAAGCTGAAGGAGGCAAAAGAGCAGCAGGCCAGCATGGAGGAGAAGTTCCACAACGAGCTGAAC GCTCACATTAAACTGAGCAACCTGTATAAATCCGCTGCCGATGATAGCGAGGCAAAAAGCAATGAACTCA CTAGAGCCGTTGAGGAGCTGCACAAGCTGCTGAAAGAGGCGGGAGAGGCTAACAAGGCGATCCAAGATCA TCTGTTGGAGGTGGAACAGAGTAAGGATCAAATGGAAAAGGAGATGTTGGAAAAAATTGGCCGCTTGGAG AAAGAACTTGAAAATGCTAACGATCTCCTGTCCGCAACAAAGCGCAAGGGGGCCATTCTCTCTGAGGAGG AGCTGGCCGCTATGTCCCCAACAGCCGCCGCGGTCGCAAAGATCGTCAAGCCTGGTATGAAGCTGACGGA GCTGTATAACGCTTATGTGGAGACACAAGACCAGCTGCTGTTGGAAAAGTTGGAGAACAAGCGCATCAAT AAGTATTTGGACGAAATTGTTAAGGAGGTGGAGGCCAAAGCGCCGATTTTGAAGCGGCAGAGAGAGGAGT ACGAGAGAGCTCAAAAAGCAGTGGCATCCCTCTCAGTGAAGCTTGAACAGGCGATGAAAGAAATCCAGAG ACTGCAGGAGGACACCGATAAAGCCAACAAACAGTCTTCCGTCCTGGAGCGAGATAACCGGAGGATGGAA ATCCAGGTGAAGGACCTCTCTCAGCAGATTCGGGTGCTTCTGATGGAACTGGAGGAGGCAAGGGGGAATC ACGTCATTAGGGACGAGGAGGTGAGCTCCGCCGACATCAGTTCTTCAAGCGAAGTTATTAGCCAACACTT GGTATCCTACAGGAATATTGAGGAGTTGCAACAGCAGAACCAGAGGCTGCTGGTGGCTCTTCGCGAACTG GGGGAGACTCGCGAACGCGAGGAACAGGAGACTACCTCTTCCAAGATAACTGAGCTGCAGTTGAAGCTCG AGTCCGCCCTCACCGAGCTTGAGCAGCTGCGGAAGTCAAGACAGCACCAGATGCAGCTTGTTGACTCCAT CGTCCGGCAAAGGGACATGTACCGCATCCTGCTGAGTCAGACCACAGGGGTAGCTATCCCCCTGCATGCC AGTAGTCTGGATGACGTCTCTCTGGCCAGTACCCCAAAGAGGCCCTCTACCAGTCAAACCGTCAGTACCC CTGCTCCGGTGCCTGTTATCGAGAGCACAGAGGCAATTGAGGCGAAGGCAGCTCTTAAGCAGCTCCAGGA AATATTCGAAAACTACAAGAAGGAGAAAGCCGAAAACGAAAAGATTCAAAACGAGCAGCTCGAGAAGCTC CAGGAGCAAGTGACTGATCTCCGCTCTCAGAACACTAAAATATCCACACAATTGGACTTCGCCTCCAAGC GGTATGAGATGCTTCAAGACAACGTGGAGGGTTACAGACGAGAGATTACCAGCCTGCATGAGAGGAACCA AAAGCTGACAGCCACCACCCAGAAACAAGAGCAAATCATTAACACCATGACGCAAGACCTGAGGGGCGCG AACGAGAAGCTGGCGGTGGCCGAGGTCCGCGCAGAGAACCTTAAGAAGGAGAAAGAGATGCTTAAACTTA GCGAGGTCAGACTGAGCCAGCAACGCGAAAGCCTGCTGGCTGAACAGAGGGGCCAGAATCTCCTCCTGAC TAATCTGCAAACAATTCAGGGTATCTTGGAGAGGTCCGAAACTGAAACAAAGCAACGGCTTTCAAGCCAG ATTGAAAAATTGGAGCACGAAATTAGCCACCTCAAGAAGAAGCTGGAGAATGAGGTGGAGCAGCGACACA CCCTCACCCGGAATCTCGACGTCCAGCTGCTTGACACTAAAAGGCAACTGGATACAGAAACCAACCTGCA CCTTAATACTAAGGAGCTGCTGAAGAACGCACAGAAAGAAATTGCTACTCTTAAGCAGCACCTCTCAAAT ATGGAGGTCCAGGTAGCCTCACAATCTTCACAGCGGACCGGAAAAGGGCAGCCGTCCAACAAAGAGGACG TGGATGACCTCGTGTCCCAGCTGCGGCAGACAGAAGAGCAGGTGAACGACCTCAAGGAACGCCTTAAAAC TTCCACTAGTAACGTTGAACAGTATCAAGCGATGGTTACCAGTCTGGAAGAGTCACTGAACAAAGAAAAG CAGGTGACCGAGGAGGTCAGGAAAAACATCGAGGTGCGCCTGAAAGAGTCTGCAGAATTCCAGACCCAGC TGGAGAAGAAACTGATGGAGGTGGAGAAGGAGAAGCAGGAGCTCCAGGATGATAAAAGGCGGGCCATCGA GTCTATGGAGCAGCAACTGTCCGAACTCAAAAAAACCTTGTCTTCTGTGCAGAATGAAGTGCAAGAGGCG TTGCAGAGAGCATCCACCGCTCTGAGTAATGAACAGCAGGCCAGACGGGACTGTCAGGAACAAGCCAAGA TCGCAGTCGAAGCTCAGAACAAGTACGAACGCGAACTGATGCTTCATGCAGCAGACGTGGAAGCACTGCA AGCCGCAAAAGAGCAGGTGTCCAAGATGGCCAGCGTACGGCAACATTTGGAAGAGACAACACAAAAGGCA GAGAGCCAGCTTTTGGAATGCAAGGCCAGCTGGGAGGAGCGCGAGAGAATGCTTAAGGACGAAGTGTCAA AGTGTGTGTGCAGATGCGAGGACCTGGAGAAACAAAATCGACTCCTCCACGATCAGATCGAGAAACTGTC CGATAAAGTTGTTGCATCAGTGAAGGAGGGGGTGCAGGGCCCTCTGAATGTGTCCCTGTCCGAGGAGGGC AAAAGTCAAGAGCAGATACTCGAAATACTCAGGTTCATCAGGCGGGAGAAAGAAATTGCAGAGACAAGAT TTGAGGTGGCCCAGGTGGAAAGTCTGCGATATAGACAAAGGGTCGAGCTCCTCGAACGCGAGCTGCAAGA ACTCCAAGATAGTCTCAACGCAGAAAGAGAGAAGGTCCAAGTTACTGCCAAAACAATGGCCCAGCACGAG GAGCTGATGAAGAAAACGGAAACGATGAATGTCGTCATGGAGACAAATAAGATGCTGAGAGAGGAAAAAG AGCGCCTGGAACAAGATCTCCAGCAAATGCAGGCGAAAGTTCGAAAACTGGAGCTTGACATCCTCCCCCT TCAGGAAGCAAACGCGGAGCTCTCAGAAAAAAGCGGCATGTTGCAAGCCGAAAAGAAATTGCTCGAAGAA GACGTGAAACGCTGGAAGGCTAGGAACCAACACCTGGTGAGCCAGCAAAAGGATCCGGATACCGAAGAAT ATCGGAAGTTGTTGTCAGAGAAGGAGGTTCACACCAAGCGCATTCAACAGCTTACCGAAGAAATAGGGAG ACTCAAGGCCGAGATCGCTCGCTCAAACGCCTCACTCACTAACAACCAAAACCTCATACAGAGTCTGAAA GAGGACCTGAATAAAGTGCGAACGGAAAAAGAAACTATCCAAAAGGATTTGGACGCAAAGATCATCGATA TCCAGGAGAAGGTAAAAACTATCACACAGGTCAAAAAGATTGGTCGCCGCTATAAGACACAGTATGAGGA ACTGAAAGCTCAACAGGACAAGGTAATGGAGACGTCTGCACAATCCTCCGGGGATCATCAGGAGCAGCAC GTTTCTGTGCAGGAGATGCAGGAGCTGAAAGAGACTCTGAATCAAGCGGAAACCAAGTCCAAGAGCCTGG AGTCCCAGGTTGAGAACCTTCAGAAGACTCTTTCTGAGAAAGAAACAGAAGCTAGGAACTTGCAGGAGCA GACAGTCCAGTTGCAAAGCGAGCTGAGCCGCCTCAGACAGGACCTGCAGGATCGCACCACTCAGGAAGAG CAGCTTCGGCAGCAGATCACCGAAAAAGAGGAGAAGACACGCAAGGCAATTGTAGCAGCAAAGAGCAAGA TCGCTCACTTGGCTGGCGTGAAAGACCAACTCACAAAGGAAAACGAGGAGTTGAAGCAGCGAAATGGAGC CTTGGATCAGCAGAAGGATGAGCTCGACGTGCGGATCACGGCCTTGAAAAGCCAGTATGAGGGGCGCATC TCAAGACTGGAACGCGAGCTGAGGGAACACCAGGAACGCCACCTGGAGCAGAGGGACGAACCACAGGAAC CGTCTAACAAAGTACCGGAGCAGCAGAGGCAGATAACCCTGAAGACCACCCCGGCATCCGGGGAGCGGGG AATCGCTTCAACTAGCGACCCTCCTACCGCGAACATAAAGCCCACACCAGTCGTTTCCACACCTAGTAAG GTGACGGCTGCCGCAATGGCCGGCAATAAAAGTACGCCAAGGGCCTCAATCCGGCCCATGGTGACCCCTG CAACGGTCACTAACCCCACAACAACACCCACCGCCACCGTAATGCCAACCACCCAAGTGGAATCACAGGA GGCCATGCAGAGCGAAGGCCCAGTGGAGCACGTGCCAGTCTTTGGCAGCACGTCTGGCTCTGTTCGGTCA ACGAGCCCGAATGTTCAGCCATCAATCTCACAGCCCATCCTGACTGTACAGCAGCAAACGCAGGCAACCG CCTTTGTCCAGCCTACTCAGCAGTCACATCCTCAGATCGAGCCGGCCAATCAAGAGCTTAGTTCCAATAT TGTAGAGGTGGTGCAGAGCTCTCCCGTGGAGAGGCCGTCTACATCCACGGCAGTCTTTGGGACCGTGAGC GCCACACCTAGTTCTTCTTTGCCTAAGCGGACCAGAGAAGAGGAGGAGGACAGTACTATAGAAGCTTCTG ATCAAGTAAGCGATGACACGGTGGAAATGCCGCTTCCCAAGAAGCTGAAGTCTGTCACCCCAGTGGGTAC CGAAGAAGAGGTCATGGCAGAGGAGAGCACCGATGGAGAGGTGGAGACACAGGTGTACAATCAGGATTCC CAAGACTCCATTGGTGAGGGCGTCACTCAGGGCGACTATACACCAATGGAGGATTCTGAGGAGACATCCC AATCTCTGCAGATAGACCTGGGCCCGCTCCAGAGTGACCAGCAAACAACCACATCCTCTCAGGACGGACA GGGCAAAGGAGACGACGTTATCGTCATTGATAGCGATGATGAAGAAGAGGATGATGATGAGAATGATGGC GAACATGAGGACTATGAAGAGGACGAGGAGGATGACGACGATGATGAAGACGATACAGGGATGGGAGACG AGGGGGAAGATAGCAACGAAGGCACAGGTAGTGCCGATGGAAATGACGGATATGAAGCTGATGACGCTGA GGGCGGAGATGGTACAGATCCCGGCACAGAGACAGAGGAGAGCATGGGTGGGGGCGAAGGGAATCATCGG GCAGCTGACTCACAGAATAGCGGCGAAGGCAATACAGGGGCTGCAGAAAGTTCTTTTTCCCAGGAAGTAT CACGAGAGCAACAGCCATCCTCTGCCAGCGAACGCCAGGCCCCTAGAGCTCCCCAGTCACCTAGGAGGCC TCCTCACCCACTGCCCCCCAGACTCACAATCCACGCACCCCCCCAAGAACTGGGTCCTCCCGTCCAGAGA ATCCAGATGACCCGGCGGCAGTCAGTAGGAAGGGGCCTGCAACTCACGCCTGGAATAGGCGGGATGCAAC AGCATTTTTTTGATGACGAGGACCGCACCGTGCCTTCAACACCAACCCTGGTGGTACCGCACCGAACCGA TGGATTCGCCGAGGCAATACATTCTCCTCAGGTAGCAGGAGTGCCAAGATTCAGATTTGGTCCTCCAGAA GACATGCCTCAAACAAGCAGTTCTCATAGCGATCTCGGACAGCTTGCAAGCCAGGGCGGGTTGGGGATGT ACGAAACCCCATTGTTTCTGGCTCACGAAGAGGAGAGCGGAGGCAGAAGCGTGCCCACTACACCTTTGCA GGTGGCCGCCCCCGTCACTGTCTTCACTGAAAGCACCACCTCTGACGCTAGCGAACACGCTTCCCAAAGT GTTCCCATGGTAACAACCAGCACCGGCACACTTAGTACAACGAATGAAACCGCGACAGGCGACGACGGAG ATGAGGTGTTTGTGGAGGCCGAATCTGAAGGTATTTCATCAGAGGCCGGATTGGAAATTGATTCCCAGCA GGAGGAGGAGCCAGTTCAAGCTAGCGACGAGTCCGATCTGCCATCCACTAGTCAGGACCCACCGTCAAGC TCCTCAGTAGATACATCATCCAGCCAGCCCAAGCCGTTCAGAAGAGTGCGGCTCCAGACCACACTTCGGC AAGGCGTGAGGGGGCGGCAGTTCAACCGACAGCGAGGTGTTTCTCATGCCATGGGCGGGAGAGGAGGAAT TAACAGGGGAAATATCAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214233 representing NM_003292
Red=Cloning site Green=Tags(s) MAAVLQQVLERTELNKLPKSVQNKLEKFLADQQSEIDGLKGRHEKFKVESEQQYFEIEKRLSHSQERLVN ETRECQSLRLELEKLNNQLKALTEKNKELEIAQDRNIAIQSQFTRTKEELEAEKRDLIRTNERLSQELEY LTEDVKRLNEKLKESNTTKGELQLKLDELQASDVSVKYREKRLEQEKELLHSQNTWLNTELKTKTDELLA LGREKGNEILELKCNLENKKEEVSRLEEQMNGLKTSNEHLQKHVEDLLTKLKEAKEQQASMEEKFHNELN AHIKLSNLYKSAADDSEAKSNELTRAVEELHKLLKEAGEANKAIQDHLLEVEQSKDQMEKEMLEKIGRLE KELENANDLLSATKRKGAILSEEELAAMSPTAAAVAKIVKPGMKLTELYNAYVETQDQLLLEKLENKRIN KYLDEIVKEVEAKAPILKRQREEYERAQKAVASLSVKLEQAMKEIQRLQEDTDKANKQSSVLERDNRRME IQVKDLSQQIRVLLMELEEARGNHVIRDEEVSSADISSSSEVISQHLVSYRNIEELQQQNQRLLVALREL GETREREEQETTSSKITELQLKLESALTELEQLRKSRQHQMQLVDSIVRQRDMYRILLSQTTGVAIPLHA SSLDDVSLASTPKRPSTSQTVSTPAPVPVIESTEAIEAKAALKQLQEIFENYKKEKAENEKIQNEQLEKL QEQVTDLRSQNTKISTQLDFASKRYEMLQDNVEGYRREITSLHERNQKLTATTQKQEQIINTMTQDLRGA NEKLAVAEVRAENLKKEKEMLKLSEVRLSQQRESLLAEQRGQNLLLTNLQTIQGILERSETETKQRLSSQ IEKLEHEISHLKKKLENEVEQRHTLTRNLDVQLLDTKRQLDTETNLHLNTKELLKNAQKEIATLKQHLSN MEVQVASQSSQRTGKGQPSNKEDVDDLVSQLRQTEEQVNDLKERLKTSTSNVEQYQAMVTSLEESLNKEK QVTEEVRKNIEVRLKESAEFQTQLEKKLMEVEKEKQELQDDKRRAIESMEQQLSELKKTLSSVQNEVQEA LQRASTALSNEQQARRDCQEQAKIAVEAQNKYERELMLHAADVEALQAAKEQVSKMASVRQHLEETTQKA ESQLLECKASWEERERMLKDEVSKCVCRCEDLEKQNRLLHDQIEKLSDKVVASVKEGVQGPLNVSLSEEG KSQEQILEILRFIRREKEIAETRFEVAQVESLRYRQRVELLERELQELQDSLNAEREKVQVTAKTMAQHE ELMKKTETMNVVMETNKMLREEKERLEQDLQQMQAKVRKLELDILPLQEANAELSEKSGMLQAEKKLLEE DVKRWKARNQHLVSQQKDPDTEEYRKLLSEKEVHTKRIQQLTEEIGRLKAEIARSNASLTNNQNLIQSLK EDLNKVRTEKETIQKDLDAKIIDIQEKVKTITQVKKIGRRYKTQYEELKAQQDKVMETSAQSSGDHQEQH VSVQEMQELKETLNQAETKSKSLESQVENLQKTLSEKETEARNLQEQTVQLQSELSRLRQDLQDRTTQEE QLRQQITEKEEKTRKAIVAAKSKIAHLAGVKDQLTKENEELKQRNGALDQQKDELDVRITALKSQYEGRI SRLERELREHQERHLEQRDEPQEPSNKVPEQQRQITLKTTPASGERGIASTSDPPTANIKPTPVVSTPSK VTAAAMAGNKSTPRASIRPMVTPATVTNPTTTPTATVMPTTQVESQEAMQSEGPVEHVPVFGSTSGSVRS TSPNVQPSISQPILTVQQQTQATAFVQPTQQSHPQIEPANQELSSNIVEVVQSSPVERPSTSTAVFGTVS ATPSSSLPKRTREEEEDSTIEASDQVSDDTVEMPLPKKLKSVTPVGTEEEVMAEESTDGEVETQVYNQDS QDSIGEGVTQGDYTPMEDSEETSQSLQIDLGPLQSDQQTTTSSQDGQGKGDDVIVIDSDDEEEDDDENDG EHEDYEEDEEDDDDDEDDTGMGDEGEDSNEGTGSADGNDGYEADDAEGGDGTDPGTETEESMGGGEGNHR AADSQNSGEGNTGAAESSFSQEVSREQQPSSASERQAPRAPQSPRRPPHPLPPRLTIHAPPQELGPPVQR IQMTRRQSVGRGLQLTPGIGGMQQHFFDDEDRTVPSTPTLVVPHRTDGFAEAIHSPQVAGVPRFRFGPPE DMPQTSSSHSDLGQLASQGGLGMYETPLFLAHEEESGGRSVPTTPLQVAAPVTVFTESTTSDASEHASQS VPMVTTSTGTLSTTNETATGDDGDEVFVEAESEGISSEAGLEIDSQQEEEPVQASDESDLPSTSQDPPSS SSVDTSSSQPKPFRRVRLQTTLRQGVRGRQFNRQRGVSHAMGGRGGINRGNIN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003292 |
| ORF Size | 7089 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003292.2, NP_003283.2 |
| RefSeq Size | 9636 bp |
| RefSeq ORF | 7092 bp |
| Locus ID | 7175 |
| UniProt ID | P12270 |
| Cytogenetics | 1q31.1 |
| Domains | M, Pox_A_type_inc |
| Protein Pathways | Pathways in cancer, Thyroid cancer |
| MW | 267.3 kDa |
| Gene Summary | This gene encodes a large coiled-coil protein that forms intranuclear filaments attached to the inner surface of nuclear pore complexes (NPCs). The protein directly interacts with several components of the NPC. It is required for the nuclear export of mRNAs and some proteins. Oncogenic fusions of the 5' end of this gene with several different kinase genes occur in some neoplasias. [provided by RefSeq, Jul 2008] |
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