NARG1L (NAA16) (NM_024561) Human Tagged ORF Clone

CAT#: RC214224

NAA16 (Myc-DDK-tagged)-Human N(alpha)-acetyltransferase 16, NatA auxiliary subunit (NAA16), transcript variant 1

Product Images

Circular map for RC214224

• 

  Western blot validation of overexpression lysate (Cat# LY403001) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214224 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NARG1L
• Synonyms: NARG1L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214224 representing NM_024561
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGAACGTGCTGCTGCCGCCCAAGGAGAGCAACCTCTTCAAACGCATCTTGAAATGTTATGAACAGA
  AGCAGTACAAAAATGGCCTCAAGTTTTGCAAGATGATTCTGTCGAACCCAAAATTTGCTGAACATGGAGA
  GACTTTGGCTATGAAAGGATTAACACTGAACTGTTTAGGAAAAAAAGAAGAAGCTTATGAGTTTGTTCGT
  AAAGGACTTCGTAATGATGTCAAGAGTCATGTCTGTTGGCATGTATATGGACTCTTGCAGCGTTCTGATA
  AAAAATATGATGAAGCTATAAAATGTTACCGAAATGCCCTCAAATTAGATAAAGATAACCTGCAAATTTT
  GAGGGATCTCTCACTGTTGCAGATCCAAATGAGAGACCTTGAAGGTTACCGAGAGACAAGATACCAGCTT
  CTTCAGTTGCGCCCCACACAGCGTGCCTCCTGGATTGGATATGCTATTGCATACCATTTGCTGAAAGATT
  ATGATATGGCCCTAAAACTGTTGGAAGAATTTAGACAAACTCAGCAAGTTCCTCCAAACAAAATAGATTA
  TGAATATAGTGAATTGATATTATACCAGAATCAAGTGATGAGAGAGGCAGATCTGTTGCAGGAATCTTTG
  GAACATATAGAAATGTATGAGAAACAAATATGTGATAAACTTTTGGTGGAAGAAATTAAAGGGGAAATAC
  TGTTGAAATTGGGAAGATTAAAAGAAGCCAGTGAAGTGTTCAAAAACTTGATTGATCGAAATGCAGAAAA
  CTGGTGTTATTATGAAGGCTTGGAAAAAGCTCTACAAATTAGCACTTTAGAAGAGAGGCTTCAAATTTAT
  GAAGAAATTAGTAAGCAGCACCCCAAAGCAATTACACCCAGAAGATTACCTTTGACTCTTGTCCCAGGTG
  AAAGATTTAGAGAACTAATGGATAAGTTCCTGAGGGTTAACTTCAGTAAAGGCTGCCCACCCTTGTTTAC
  TACTTTGAAATCTTTATATTACAATACAGAAAAGGTTTCTATAATCCAGGAACTTGTTACTAATTATGAA
  GCCTCTCTTAAAACGTGTGACTTTTTTAGCCCATATGAGAATGGGGAGAAGGAACCCCCGACAACACTAC
  TCTGGGTTCAGTATTTCCTGGCACAGCACTTTGATAAACTTGGACAGTATTCTTTGGCTTTGGATTATAT
  TAATGCTGCAATTGCTAGTACTCCAACTCTAATAGAATTATTCTATATGAAAGCAAAAATTTACAAGCAT
  ATAGGTAATCTCAAAGAAGCTGCAAAGTGGATGGATGAAGCACAGTCTTTGGACACAGCTGATAGATTCA
  TCAATTCCAAATGTGCAAAATACATGCTTCGAGCAAATATGATAAAAGAAGCAGAGGAAATGTGCTCCAA
  GTTCACAAGGGAAGGAACATCTGCCATGGAAAATCTAAATGAAATGCAGTGTATGTGGTTTCAGACAGAA
  TGCATTTCAGCTTATCAGCGTCTGGGGAGATACGGGGATGCCTTGAAAAAATGTCATGAAGTAGAAAGGC
  ATTTTTTTGAGATAACTGATGACCAATTCGACTTCCATACATACTGCATGAGAAAGATGACCCTTCGTGC
  CTATGTTGACCTTTTGAGATTAGAAGATATACTCAGAAGACATGCCTTTTATTTCAAGGCTGCTAGATCA
  GCGATTGAAATATACTTGAAATTGTATGATAATCCCTTAACCAATGAAAGCAAACAACAAGAAATAAACT
  CAGAAAACTTGTCAGCCAAAGAATTGAAGAAAATGCTTAGCAAGCAGAGAAGAGCTCAGAAAAAGGCTAA
  ACTAGAAGAAGAAAGAAAGCATGCAGAAAGAGAACGTCAACAGAAAAATCAAAAGAAAAAAAGAGATGAA
  GAAGAAGAAGAAGCCAGTGGCCTTAAGGAAGAACTTATACCTGAAAAATTAGAAAGGGTAGAAAATCCAT
  TAGAGGAAGCCGTTAAGTTCCTTATACCTCTTAAGAACCTTGTTGCTGATAACATTGACACTCATCTGTT
  AGCATTTGAAATATATTTTAGAAAAGGAAAGTTTCTGTTAATGCTGCAGTCTGTCAAACGAGCTTTTGCC
  ATTAACAGTAATAACCCATGGTTACATGAATGTTTAATTAGATTTTCTAAATCTGTGTCTAATCATAGTA
  ATCTTCCAGACATTGTGAGCAAAGTTCTATCTCAAGAAATGCAGAAAATATTTGTCAAAAAGGATTTGGA
  AAGTTTTAATGAGGATTTTCTGAAACGTAACGCTACCTCTCTTCAGCATCTACTTTCAGGTGCTAAAATG
  ATGTATTTTCTGGACAAGTCAAGGCAGGAGAAAGCAATTGCTATAGCCACTAGACTAGATGAAACTATAA
  AAGATAAAGATGTAAAGACATTAATAAAGGTTTCTGAAGCACTGCTTGATGGCAGCTTTGGGAACTGTAG
  TTCCCAATATGAAGAATATAGGATGGCCTGTCATAACCTGCTTCCTTTTACATCTGCCTTCTTGCCTGCT
  GTGAATGAAGTCGACAATCCTAATGTGGCACTGAACCATACAGCTAATTATGATGTCTTGGCAAATGAAA
  TT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214224 representing NM_024561
  Red=Cloning site Green=Tags(s)
  
  MPNVLLPPKESNLFKRILKCYEQKQYKNGLKFCKMILSNPKFAEHGETLAMKGLTLNCLGKKEEAYEFVR
  KGLRNDVKSHVCWHVYGLLQRSDKKYDEAIKCYRNALKLDKDNLQILRDLSLLQIQMRDLEGYRETRYQL
  LQLRPTQRASWIGYAIAYHLLKDYDMALKLLEEFRQTQQVPPNKIDYEYSELILYQNQVMREADLLQESL
  EHIEMYEKQICDKLLVEEIKGEILLKLGRLKEASEVFKNLIDRNAENWCYYEGLEKALQISTLEERLQIY
  EEISKQHPKAITPRRLPLTLVPGERFRELMDKFLRVNFSKGCPPLFTTLKSLYYNTEKVSIIQELVTNYE
  ASLKTCDFFSPYENGEKEPPTTLLWVQYFLAQHFDKLGQYSLALDYINAAIASTPTLIELFYMKAKIYKH
  IGNLKEAAKWMDEAQSLDTADRFINSKCAKYMLRANMIKEAEEMCSKFTREGTSAMENLNEMQCMWFQTE
  CISAYQRLGRYGDALKKCHEVERHFFEITDDQFDFHTYCMRKMTLRAYVDLLRLEDILRRHAFYFKAARS
  AIEIYLKLYDNPLTNESKQQEINSENLSAKELKKMLSKQRRAQKKAKLEEERKHAERERQQKNQKKKRDE
  EEEEASGLKEELIPEKLERVENPLEEAVKFLIPLKNLVADNIDTHLLAFEIYFRKGKFLLMLQSVKRAFA
  INSNNPWLHECLIRFSKSVSNHSNLPDIVSKVLSQEMQKIFVKKDLESFNEDFLKRNATSLQHLLSGAKM
  MYFLDKSRQEKAIAIATRLDETIKDKDVKTLIKVSEALLDGSFGNCSSQYEEYRMACHNLLPFTSAFLPA
  VNEVDNPNVALNHTANYDVLANEI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024561
• ORF Size: 2592 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024561.5
• RefSeq Size: 4266 bp
• RefSeq ORF: 2595 bp
• Locus ID: 79612
• UniProt ID: Q6N069
• Cytogenetics: 13q14.11
• Protein Families: Druggable Genome, Transcription Factors
• MW: 101.3 kDa
• Gene Summary: Auxillary subunit of the N-terminal acetyltransferase A (NatA) complex which displays alpha (N-terminal) acetyltransferase activity.[UniProtKB/Swiss-Prot Function]