ORP150 (HYOU1) (NM_006389) Human Tagged ORF Clone

CAT#: RC214178

HYOU1 (Myc-DDK-tagged)-Human hypoxia up-regulated 1 (HYOU1), transcript variant 1

Product Images

Circular map for RC214178

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORP150
• Synonyms: GRP-170; Grp170; HSP12A; IMD59; ORP-150; ORP150
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214178 representing NM_006389
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGACAAAGTTAGGAGGCAGAGGCCGAGGAGGCGAGTCTGTTGGGCCTTGGTGGCTGTGCTCTTGG
  CAGACCTGTTGGCACTGAGTGATACACTGGCAGTGATGTCTGTGGACCTGGGCAGTGAGTCCATGAAGGT
  GGCCATTGTCAAACCTGGAGTGCCCATGGAAATTGTCTTGAATAAGGAATCTCGGAGGAAAACACCGGTG
  ATCGTGACCCTGAAAGAAAATGAAAGATTCTTTGGAGACAGTGCAGCAAGCATGGCGATTAAGAATCCAA
  AGGCTACGCTACGTTACTTCCAGCACCTCCTGGGGAAGCAGGCAGATAACCCCCATGTAGCTCTTTACCA
  GGCCCGCTTCCCGGAGCACGAGCTGACTTTCGACCCACAGAGGCAGACTGTGCACTTTCAGATCAGCTCG
  CAGCTGCAGTTCTCACCTGAGGAAGTGTTGGGCATGGTTCTCAATTATTCTCGTTCTCTAGCTGAAGATT
  TTGCAGAGCAGCCCATCAAGGATGCAGTGATCACCGTGCCAGTCTTCTTCAACCAGGCCGAGCGCCGAGC
  TGTGCTGCAGGCTGCTCGTATGGCTGGCCTCAAAGTGCTGCAGCTCATCAATGACAACACCGCCACTGCC
  CTCAGCTATGGTGTCTTCCGCCGGAAAGATATTAACACCACTGCCCAGAATATCATGTTCTATGACATGG
  GCTCAGGCAGCACCGTATGCACCATTGTGACCTACCAGATGGTGAAGACTAAGGAAGCTGGGATGCAGCC
  ACAGCTGCAGATCCGGGGAGTAGGATTTGACCGTACCCTGGGGGGCCTGGAGATGGAGCTCCGGCTTCGA
  GAACGCCTGGCTGGGCTTTTCAATGAGCAGCGCAAGGGTCAGAGAGCAAAGGATGTGCGGGAGAACCCGC
  GTGCCATGGCCAAGCTGCTGCGTGAGGCTAATCGGCTCAAAACCGTCCTCAGTGCCAACGCTGACCACAT
  GGCACAGATTGAAGGCCTGATGGATGATGTGGACTTCAAGGCAAAAGTGACTCGTGTGGAATTTGAGGAG
  TTGTGTGCAGACTTGTTTGAGCGGGTGCCTGGGCCTGTACAGCAGGCCCTCCAGAGTGCCGAAATGAGTC
  TGGATGAGATTGAGCAGGTGATCCTGGTGGGTGGGGCCACTCGGGTCCCCAGAGTTCAGGAGGTGCTGCT
  GAAGGCCGTGGGCAAGGAGGAGCTGGGGAAGAACATCAATGCAGATGAAGCAGCCGCCATGGGGGCAGTG
  TACCAGGCAGCTGCGCTCAGCAAAGCCTTTAAAGTGAAGCCATTTGTCGTCCGAGATGCAGTGGTCTACC
  CCATCCTGGTGGAGTTCACGAGGGAGGTGGAGGAGGAGCCTGGGATTCACAGCCTGAAGCACAATAAACG
  GGTACTCTTCTCTCGGATGGGGCCCTACCCTCAACGCAAAGTCATCACCTTTAACCGCTACAGCCATGAT
  TTCAACTTCCACATCAACTACGGCGACCTGGGCTTCCTGGGGCCTGAAGATCTTCGGGTATTTGGCTCCC
  AGAATCTGACCACAGTGAAGCTAAAAGGGGTGGGTGACAGCTTCAAGAAGTATCCTGACTACGAGTCCAA
  GGGCATCAAGGCTCACTTCAACCTGGATGAGAGTGGCGTGCTCAGTCTAGACAGGGTGGAGTCTGTATTT
  GAGACACTGGTAGAGGACAGCGCAGAAGAGGAATCTACTCTCACCAAACTTGGCAACACCATTTCCAGCC
  TGTTTGGAGGCGGTACCACACCAGATGCCAAGGAGAATGGTACTGATACTGTCCAGGAGGAAGAGGAGAG
  CCCTGCAGAGGGGAGCAAGGACGAGCCTGGGGAGCAGGTGGAGCTCAAGGAGGAAGCTGAGGCCCCAGTG
  GAGGATGGCTCTCAGCCCCCACCCCCTGAACCTAAGGGAGATGCAACCCCTGAGGGAGAAAAGGCCACAG
  AAAAAGAAAATGGGGACAAGTCTGAGGCCCAGAAACCAAGTGAGAAGGCAGAGGCAGGGCCTGAGGGCGT
  CGCTCCAGCCCCAGAGGGAGAGAAGAAGCAGAAGCCCGCCAGGAAGCGGCGAATGGTAGAGGAGATCGGG
  GTGGAGCTGGTTGTTCTGGACCTGCCTGACTTGCCAGAGGATAAGCTGGCTCAGTCGGTGCAGAAACTTC
  AGGACTTGACACTCCGAGACCTGGAGAAGCAGGAACGGGAAAAAGCTGCCAACAGCTTGGAAGCGTTCAT
  ATTTGAGACCCAGGACAAGCTGTACCAGCCCGAGTACCAGGAAGTGTCCACAGAGGAGCAGCGTGAGGAG
  ATCTCTGGGAAGCTCAGCGCCGCATCCACCTGGCTGGAGGATGAGGGTGTTGGAGCCACCACAGTGATGT
  TGAAGGAGAAGCTGGCTGAGCTGAGGAAGCTGTGCCAAGGGCTGTTTTTTCGGGTAGAGGAGCGCAAGAA
  GTGGCCCGAACGGCTGTCTGCCCTCGATAATCTCCTCAACCATTCCAGCATGTTCCTCAAGGGGGCCCGG
  CTCATCCCAGAGATGGACCAGATCTTCACTGAGGTGGAGATGACAACGTTAGAGAAAGTCATCAATGAGA
  CCTGGGCCTGGAAGAATGCAACTCTGGCCGAGCAGGCTAAGCTGCCCGCCACAGAGAAGCCTGTGTTGCT
  CTCAAAAGACATTGAAGCTAAGATGATGGCCCTGGACCGAGAGGTGCAGTATCTGCTCAATAAGGCCAAG
  TTTACCAAGCCCCGGCCCCGGCCTAAGGACAAGAATGGGACCCGGGCAGAGCCACCCCTCAATGCCAGTG
  CCAGTGACCAGGGGGAGAAGGTCATCCCTCCAGCAGGCCAGACTGAAGATGCAGAGCCCATTTCAGAACC
  TGAGAAAGTAGAGACTGGATCCGAGCCAGGAGACACTGAGCCTTTGGAGTTAGGAGGTCCTGGAGCAGAA
  CCTGAACAGAAAGAACAATCGACAGGACAGAAGCGGCCTTTGAAGAACGACGAACTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214178 representing NM_006389
  Red=Cloning site Green=Tags(s)
  
  MADKVRRQRPRRRVCWALVAVLLADLLALSDTLAVMSVDLGSESMKVAIVKPGVPMEIVLNKESRRKTPV
  IVTLKENERFFGDSAASMAIKNPKATLRYFQHLLGKQADNPHVALYQARFPEHELTFDPQRQTVHFQISS
  QLQFSPEEVLGMVLNYSRSLAEDFAEQPIKDAVITVPVFFNQAERRAVLQAARMAGLKVLQLINDNTATA
  LSYGVFRRKDINTTAQNIMFYDMGSGSTVCTIVTYQMVKTKEAGMQPQLQIRGVGFDRTLGGLEMELRLR
  ERLAGLFNEQRKGQRAKDVRENPRAMAKLLREANRLKTVLSANADHMAQIEGLMDDVDFKAKVTRVEFEE
  LCADLFERVPGPVQQALQSAEMSLDEIEQVILVGGATRVPRVQEVLLKAVGKEELGKNINADEAAAMGAV
  YQAAALSKAFKVKPFVVRDAVVYPILVEFTREVEEEPGIHSLKHNKRVLFSRMGPYPQRKVITFNRYSHD
  FNFHINYGDLGFLGPEDLRVFGSQNLTTVKLKGVGDSFKKYPDYESKGIKAHFNLDESGVLSLDRVESVF
  ETLVEDSAEEESTLTKLGNTISSLFGGGTTPDAKENGTDTVQEEEESPAEGSKDEPGEQVELKEEAEAPV
  EDGSQPPPPEPKGDATPEGEKATEKENGDKSEAQKPSEKAEAGPEGVAPAPEGEKKQKPARKRRMVEEIG
  VELVVLDLPDLPEDKLAQSVQKLQDLTLRDLEKQEREKAANSLEAFIFETQDKLYQPEYQEVSTEEQREE
  ISGKLSAASTWLEDEGVGATTVMLKEKLAELRKLCQGLFFRVEERKKWPERLSALDNLLNHSSMFLKGAR
  LIPEMDQIFTEVEMTTLEKVINETWAWKNATLAEQAKLPATEKPVLLSKDIEAKMMALDREVQYLLNKAK
  FTKPRPRPKDKNGTRAEPPLNASASDQGEKVIPPAGQTEDAEPISEPEKVETGSEPGDTEPLELGGPGAE
  PEQKEQSTGQKRPLKNDEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006389
• ORF Size: 2997 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006389.2, NP_006380.1
• RefSeq Size: 4552 bp
• RefSeq ORF: 3000 bp
• Locus ID: 10525
• UniProt ID: Q9Y4L1
• Cytogenetics: 11q23.3
• Domains: HSP70
• Protein Families: Transmembrane
• MW: 111.34 kDa
• Gene Summary: The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]