GABRE (NM_004961) Human Tagged ORF Clone

CAT#: RC214063

GABRE (Myc-DDK-tagged)-Human gamma-aminobutyric acid (GABA) A receptor, epsilon (GABRE)

Product Images

Circular map for RC214063

• 

  Western blot validation of overexpression lysate (Cat# LY401542) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214063 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GABRE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214063 representing NM_004961
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTCCAAAGTTCTTCCAGTCTTCCTAGGCATCTTATTGATCCTCCAGTCGAGGGTCGAGGGACCTC
  AGACTGAATCAAAGAATGAAGCCTCTTCCCGTGATGTTGTCTATGGCCCCCAGCCCCAGCCTCTGGAAAA
  TCAGCTCCTCTCTGAGGAAACAAAGTCAACTGAGACTGAGACTGGGAGCAGAGTTGGCAAACTGCCAGAA
  GCCTCTCGCATCCTGAACACTATCCTGAGTAATTATGACCACAAACTGCGCCCTGGCATTGGAGAGAAGC
  CCACTGTGGTCACTGTTGAGATCGCCGTCAACAGCCTTGGTCCTCTCTCTATCCTAGACATGGAATACAC
  CATTGACATCATCTTCTCCCAGACCTGGTACGACGAACGCCTCTGTTACAACGACACCTTTGAGTCTCTT
  GTTCTGAATGGCAATGTGGTGAGCCAGCTATGGATCCCGGACACCTTTTTTAGGAATTCTAAGAGGACCC
  ACGAGCATGAGATCACCATGCCCAACCAGATGGTCCGCATCTACAAGGATGGCAAGGTGTTGTACACAAT
  TAGGATGACCATTGATGCCGGATGCTCACTCCACATGCTCAGATTTCCAATGGATTCTCACTCTTGCCCT
  CTATCTTTCTCTAGCTTTTCCTATCCTGAGAATGAGATGATCTACAAGTGGGAAAATTTCAAGCTTGAAA
  TCAATGAGAAGAACTCCTGGAAGCTCTTCCAGTTTGATTTTACAGGAGTGAGCAACAAAACTGAAATAAT
  CACAACCCCAGTTGGTGACTTCATGGTCATGACGATTTTCTTCAATGTGAGCAGGCGGTTTGGCTATGTT
  GCCTTTCAAAACTATGTCCCTTCTTCCGTGACCACGATGCTCTCCTGGGTTTCCTTTTGGATCAAGACAG
  AGTCTGCTCCAGCCCGGACCTCTCTAGGGATCACCTCTGTTCTGACCATGACCACGTTGGGCACCTTTTC
  TCGTAAGAATTTCCCGCGTGTCTCCTATATCACAGCCTTGGATTTCTATATCGCCATCTGCTTCGTCTTC
  TGCTTCTGCGCTCTGTTGGAGTTTGCTGTGCTCAACTTCCTGATCTACAACCAGACAAAAGCCCATGCTT
  CTCCTAAACTCCGCCATCCTCGTATCAATAGCCGTGCCCATGCCCGTACCCGTGCACGTTCCCGAGCCTG
  TGCCCGCCAACATCAGGAAGCTTTTGTGTGCCAGATTGTCACCACTGAGGGAAGTGATGGAGAGGAGCGC
  CCGTCTTGCTCAGCCGAGCAGCCCCCTAGCCCAGGTAGCCCTGAGGGTCCCCGCAGCCTCTGCTCCAAGC
  TGGCCTGCTGTGAGTGGTGCAAGCGTTTTAAGAAGTACTTCTGCATGGTCCCCGATTGTGAGGGCAGTAC
  CTGGCAGCAGGGCCGCCTCTGCATCCATGTCTACCGCCTGGATAACTACTCGAGAGTTGTTTTCCCAGTG
  ACTTTCTTCTTCTTCAATGTGCTCTACTGGCTTGTTTGCCTTAACTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214063 representing NM_004961
  Red=Cloning site Green=Tags(s)
  
  MLSKVLPVFLGILLILQSRVEGPQTESKNEASSRDVVYGPQPQPLENQLLSEETKSTETETGSRVGKLPE
  ASRILNTILSNYDHKLRPGIGEKPTVVTVEIAVNSLGPLSILDMEYTIDIIFSQTWYDERLCYNDTFESL
  VLNGNVVSQLWIPDTFFRNSKRTHEHEITMPNQMVRIYKDGKVLYTIRMTIDAGCSLHMLRFPMDSHSCP
  LSFSSFSYPENEMIYKWENFKLEINEKNSWKLFQFDFTGVSNKTEIITTPVGDFMVMTIFFNVSRRFGYV
  AFQNYVPSSVTTMLSWVSFWIKTESAPARTSLGITSVLTMTTLGTFSRKNFPRVSYITALDFYIAICFVF
  CFCALLEFAVLNFLIYNQTKAHASPKLRHPRINSRAHARTRARSRACARQHQEAFVCQIVTTEGSDGEER
  PSCSAEQPPSPGSPEGPRSLCSKLACCEWCKRFKKYFCMVPDCEGSTWQQGRLCIHVYRLDNYSRVVFPV
  TFFFFNVLYWLVCLNL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004961
• ORF Size: 1518 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004961.4
• RefSeq Size: 3167 bp
• RefSeq ORF: 1521 bp
• Locus ID: 2564
• UniProt ID: P78334
• Cytogenetics: Xq28
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 58 kDa
• Gene Summary: The product of this gene belongs to the ligand-gated ionic channel (TC 1.A.9) family. It encodes the gamma-aminobutyric acid (GABA) A receptor which is a multisubunit chloride channel that mediates the fastest inhibitory synaptic transmission in the central nervous system. This gene encodes an epsilon subunit. It is mapped to chromosome Xq28 in a cluster comprised of genes encoding alpha 3, beta 4 and theta subunits of the same receptor. Alternatively spliced transcript variants have been identified, but only one is thought to encode a protein. [provided by RefSeq, Oct 2008]