BACH2 (NM_021813) Human Tagged ORF Clone

CAT#: RC214061

BACH2 (Myc-DDK-tagged)-Human BTB and CNC homology 1, basic leucine zipper transcription factor 2 (BACH2), transcript variant 1

Product Images

Circular map for RC214061

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  Western blot validation of overexpression lysate (Cat# LY402878) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214061 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified BACH2 protein (Cat# TP314061). The protein was produced from HEK293T cells transfected with BACH2 cDNA clone (Cat# RC214061) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BACH2
• Synonyms: BTBD25; IMD60
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214061 representing NM_021813
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTGGATGAGAAGCCTGACTCCCCCATGTATGTGTATGAGTCCACAGTCCACTGCACCAACATCC
  TCCTGGGCCTCAATGACCAGCGGAAAAAGGATATTCTCTGTGACGTGACTTTGATCGTGGAGAGGAAGGA
  GTTCCGGGCCCACCGGGCTGTGCTGGCCGCATGCAGTGAATATTTTTGGCAGGCGCTGGTTGGACAGACA
  AAAAATGATTTGGTGGTCAGCTTGCCTGAGGAGGTCACAGCCAGGGGCTTTGGGCCGCTGTTACAGTTTG
  CCTACACTGCCAAGCTGTTACTCAGCAGAGAAAACATCCGCGAGGTCATCCGCTGTGCTGAGTTCCTGCG
  CATGCACAACCTGGAGGACTCCTGCTTCAGCTTCCTGCAGACCCAGCTCCTGAACAGTGAGGATGGCCTG
  TTTGTGTGCCGGAAGGATGCTGCGTGCCAGCGCCCACACGAGGACTGCGAGAACTCTGCAGGAGAGGAGG
  AGGATGAAGAGGAGGAGACGATGGATTCAGAGACGGCCAAGATGGCTTGCCCCAGGGACCAGATGCTTCC
  AGAGCCCATCAGCTTTGAGGCCGCCGCCATCCCCGTAGCAGAGAAGGAAGAAGCCCTGCTGCCCGAGCCT
  GACGTGCCCACAGACACCAAGGAGAGCTCAGAAAAGGACGCGTTAACGCAGTACCCCAGATACAAGAAAT
  ACCAGCTTGCATGTACCAAGAATGTCTATAATGCATCATCACACAGTACCTCAGGTTTTGCAAGCACATT
  CCGGGAAGATAACTCTAGCAACAGCCTCAAGCCGGGGCTTGCCAGGGGGCAGATTAAAAGTGAGCCGCCC
  AGTGAAGAGAATGAGGAAGAGAGCATCACGCTCTGCCTGTCTGGAGATGAGCCTGACGCCAAGGACAGAG
  CGGGGGATGTCGAGATGGACCGGAAACAGCCCAGCCCTGCCCCTACCCCCACGGCCCCAGCTGGGGCCGC
  CTGCCTGGAGAGATCCAGGAGCGTGGCCTCGCCCTCCTGCTTAAGGTCTCTGTTCAGCATAACGAAAAGT
  GTGGAGCTGTCTGGCCTGCCCAGTACATCTCAGCAGCACTTTGCCAGGAGTCCAGCCTGCCCTTTTGACA
  AGGGGATCACTCAGGGTGACCTTAAAACTGACTACACCCCTTTCACAGGGAATTATGGACAGCCCCACGT
  GGGCCAGAAGGAGGTGTCCAACTTCACCATGGGGTCGCCCCTCAGGGGGCCTGGGTTGGAGGCTCTCTGT
  AAACAGGAGGGAGAGCTGGACCGGAGGAGCGTGATCTTCTCCTCCAGCGCTTGTGACCAAGTGAGCACCT
  CGGTGCATTCTTATTCTGGGGTGAGCAGTTTGGACAAAGACCTCTCTGAGCCGGTGCCAAAGGGTCTGTG
  GGTGGGAGCCGGCCAGTCCCTCCCCAGCTCGCAGGCCTACTCCCACGGTGGGCTGATGGCCGACCACTTG
  CCAGGAAGGATGCGGCCCAACACCAGCTGCCCGGTGCCAATCAAAGTCTGCCCTCGCTCACCCCCCTTGG
  AGACCAGGACCAGGACTTCCAGCTCCTGCTCTTCCTATTCCTACGCGGAGGACGGGAGCGGGGGCTCACC
  CTGCAGCCTCCCTCTCTGTGAGTTCTCCTCCTCGCCCTGTTCCCAGGGAGCCAGATTCCTTGCCACAGAA
  CATCAGGAACCAGGCCTGATGGGAGATGGAATGTACAACCAAGTGCGGCCCCAAATTAAATGTGAGCAGT
  CTTATGGAACCAACTCCAGTGACGAATCCGGATCGTTCTCGGAAGCAGACAGTGAGTCGTGTCCTGTGCA
  GGACAGGGGCCAGGAGGTAAAACTTCCTTTTCCTGTAGATCAAATCACAGATCTTCCAAGGAACGATTTC
  CAGATGATGATTAAAATGCACAAGCTAACCTCAGAACAGTTAGAGTTTATTCATGATGTCCGACGGCGCA
  GCAAGAACCGCATCGCGGCCCAGCGCTGCCGCAAAAGGAAACTGGACTGTATTCAGAATTTAGAATGTGA
  AATCCGCAAATTGGTGTGTGAGAAAGAGAAACTGTTGTCAGAGAGGAATCAACTGAAAGCATGCATGGGG
  GAACTGTTGGACAACTTCTCCTGCCTTTCCCAGGAAGTTTGCCGAGACATCCAGAGCCCCGAGCAGATCC
  AGGCCCTGCATCGGTATTGCCCTGTCCTCAGACCCATGGACTTGCCCACGGCCTCCAGTATTAACCCTGC
  GCCCTTGGGTGCTGAGCAGAACATTGCGGCCTCCCAATGCGCAGTGGGGGAAAACGTGCCCTGCTGCTTG
  GAGCCAGGCGCGGCTCCCCCCGGACCCCCCTGGGCACCCAGCAACACCTCCGAGAATTGTACCTCTGGGA
  GGAGACTAGAAGGCACTGACCCGGGAACCTTCTCAGAGAGAGGACCTCCTCTTGAACCCAGGAGCCAAAC
  AGTGACCGTGGACTTCTGCCAGGAAATGACTGATAAGTGTACAACTGACGAACAGCCCAGGAAAGATTAT
  ACC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214061 representing NM_021813
  Red=Cloning site Green=Tags(s)
  
  MSVDEKPDSPMYVYESTVHCTNILLGLNDQRKKDILCDVTLIVERKEFRAHRAVLAACSEYFWQALVGQT
  KNDLVVSLPEEVTARGFGPLLQFAYTAKLLLSRENIREVIRCAEFLRMHNLEDSCFSFLQTQLLNSEDGL
  FVCRKDAACQRPHEDCENSAGEEEDEEEETMDSETAKMACPRDQMLPEPISFEAAAIPVAEKEEALLPEP
  DVPTDTKESSEKDALTQYPRYKKYQLACTKNVYNASSHSTSGFASTFREDNSSNSLKPGLARGQIKSEPP
  SEENEEESITLCLSGDEPDAKDRAGDVEMDRKQPSPAPTPTAPAGAACLERSRSVASPSCLRSLFSITKS
  VELSGLPSTSQQHFARSPACPFDKGITQGDLKTDYTPFTGNYGQPHVGQKEVSNFTMGSPLRGPGLEALC
  KQEGELDRRSVIFSSSACDQVSTSVHSYSGVSSLDKDLSEPVPKGLWVGAGQSLPSSQAYSHGGLMADHL
  PGRMRPNTSCPVPIKVCPRSPPLETRTRTSSSCSSYSYAEDGSGGSPCSLPLCEFSSSPCSQGARFLATE
  HQEPGLMGDGMYNQVRPQIKCEQSYGTNSSDESGSFSEADSESCPVQDRGQEVKLPFPVDQITDLPRNDF
  QMMIKMHKLTSEQLEFIHDVRRRSKNRIAAQRCRKRKLDCIQNLECEIRKLVCEKEKLLSERNQLKACMG
  ELLDNFSCLSQEVCRDIQSPEQIQALHRYCPVLRPMDLPTASSINPAPLGAEQNIAASQCAVGENVPCCL
  EPGAAPPGPPWAPSNTSENCTSGRRLEGTDPGTFSERGPPLEPRSQTVTVDFCQEMTDKCTTDEQPRKDY
  T
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021813
• ORF Size: 2523 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021813.1, NP_068585.1
• RefSeq Size: 9120 bp
• RefSeq ORF: 2526 bp
• Locus ID: 60468
• UniProt ID: Q9BYV9
• Cytogenetics: 6q15
• Domains: BTB, BRLZ
• Protein Families: Druggable Genome, Transcription Factors
• MW: 92.4 kDa
• Gene Summary: Transcriptional regulator that acts as repressor or activator (By similarity). Binds to Maf recognition elements (MARE) (By similarity). Plays an important role in coordinating transcription activation and repression by MAFK (By similarity). Induces apoptosis in response to oxidative stress through repression of the antiapoptotic factor HMOX1 (PubMed:17018862). Positively regulates the nuclear import of actin (By similarity).[UniProtKB/Swiss-Prot Function]