Apolipoprotein O like (APOOL) (NM_198450) Human Tagged ORF Clone

CAT#: RC214060

APOOL (Myc-DDK-tagged)-Human apolipoprotein O-like (APOOL)

Product Images

Circular map for RC214060

• 

  Western blot validation of overexpression lysate (Cat# LY404937) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214060 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Apolipoprotein O like
• Synonyms: CXorf33; FAM121A; Mic27; MICOS27; UNQ8193
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214060 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCATCAGGATGGGAAAACTGACAACCATGCCTGCAGGTCTGATATATGCATCTGTAAGTGTAC
  ATGCAGCCAAACAAGAGGAATCCAAAAAGCAGCTAGTGAAACCAGAGCAGCTCCCCATATATACTGCACC
  ACCGCTCCAGTCTAAATATGTTGAAGAGCAGCCTGGTCATTTACAAATGGGCTTTGCTTCCATCCGCACT
  GCAACTGGTTGTTACATTGGCTGGTGCAAGGGTGTTTATGTCTTTGTGAAAAATGGGATAATGGATACAG
  TACAATTTGGAAAAGATGCTTATGTCTATCTGAAGAATCCTCCTCGAGATTTTCTTCCGAAAATGGGAGT
  TATTACAGTTTCAGGATTGGCGGGCTTGGTTTCAGCGAGAAAAGGTTCCAAGTTTAAGAAAATTACTTAT
  CCTCTGGGACTGGCCACTTTAGGAGCAACTGTTTGCTACCCAGTTCAGTCCGTAATAATTGCTAAGGTAA
  CAGCAAAAAAGGTATATGCTACAAGCCAGCAAATTTTTGGAGCAGTTAAATCATTGTGGACAAAAAGCAG
  CAAAGAAGAGTCACTCCCTAAACCTAAAGAAAAAACTAAGCTAGGATCCTCTTCCGAAATAGAAGTACCT
  GCAAAAACAACTCACGTCTTGAAACACTCAGTGCCCTTGCCAACAGAACTCAGCTCTGAAGCAAAGACCA
  AATCAGAATCCACTTCAGGTGCAACCCAGTTTATGCCTGACCCCAAGCTCATGGATCACGGGCAGTCCCA
  CCCAGAAGATATAGATATGTACAGCACTAGAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214060 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAIRMGKLTTMPAGLIYASVSVHAAKQEESKKQLVKPEQLPIYTAPPLQSKYVEEQPGHLQMGFASIRT
  ATGCYIGWCKGVYVFVKNGIMDTVQFGKDAYVYLKNPPRDFLPKMGVITVSGLAGLVSARKGSKFKKITY
  PLGLATLGATVCYPVQSVIIAKVTAKKVYATSQQIFGAVKSLWTKSSKEESLPKPKEKTKLGSSSEIEVP
  AKTTHVLKHSVPLPTELSSEAKTKSESTSGATQFMPDPKLMDHGQSHPEDIDMYSTRS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198450
• ORF Size: 804 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198450.6
• RefSeq Size: 6479 bp
• RefSeq ORF: 807 bp
• Locus ID: 139322
• UniProt ID: Q6UXV4
• Cytogenetics: Xq21.1
• MW: 29.2 kDa
• Gene Summary: This gene encodes a protein which contains an apolipoprotein O superfamily domain. This domain is found on proteins in circulating lipoprotein complexes. [provided by RefSeq, Sep 2011]