USP47 (NM_017944) Human Tagged ORF Clone

CAT#: RC214048

USP47 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 47 (USP47)

Product Images

Circular map for RC214048

• 

  Western blot validation of overexpression lysate (Cat# LY413428) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214048 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: USP47
• Synonyms: TRFP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214048 representing NM_017944
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCCCGGCGAGGAGAACCAACTGGTCCCGAAAGAGGCACCACTGGATCATACCAGTGACAAGTCAC
  TTCTCGACGCTAATTTTGAGCCAGGAAAGAAGAACTTTCTGCATTTGACAGATAAAGATGGTGAACAACC
  TCAAATACTGCTGGAGGATTCCAGTGCTGGGGAAGACAGTGTTCATGACAGGTTTATAGGTCCGCTTCCA
  AGAGAAGGTTCTGGGGGTTCTACCAGTGATTATGTCAGCCAAAGCTACTCCTACTCATCTATTTTGAATA
  AATCAGAAACTGGATATGTGGGACTAGTAAACCAAGCAATGACTTGCTATTTGAATAGCCTTTTGCAAAC
  ACTTTTTATGACTCCTGAATTTAGGAATGCATTATATAAGTGGGAATTTGAAGAATCTGAAGAAGATCCA
  GTGACAAGTATTCCATACCAACTTCAAAGGCTTTTTGTTTTGTTACAAACCAGCAAAAAGAGAGCAATTG
  AAACCACAGATGTTACAAGGAGCTTTGGATGGGATAGTAGTGAGGCTTGGCAGCAGCATGATGTACAAGA
  ACTATGCAGAGTCATGTTTGATGCTTTGGAACAGAAATGGAAGCAAACAGAACAGGCTGATCTTATAAAT
  GAGCTATATCAAGGCAAGCTGAAGGACTACGTGAGATGTCTGGAATGTGGTTATGAGGGCTGGCGAATCG
  ACACATATCTTGATATTCCATTGGTCATCCGACCTTATGGGTCCAGCCAAGCATTTGCTAGTGTGGAAGA
  AGCATTGCATGCATTTATTCAGCCAGAGATTCTGGATGGCCCAAATCAGTATTTTTGTGAACGTTGTAAG
  AAGAAGTGTGATGCACGGAAGGGCCTTCGGTTTTTGCATTTTCCTTATCTGCTGACCTTACAGCTGAAAA
  GATTCGATTTTGATTATACAACCATGCATAGGATTAAACTGAATGATCGAATGACATTTCCCGAGGAACT
  AGATATGAGTACTTTTATTGATGTTGAAGATGAGAAATCTCCTCAGACTGAAAGTTGCACTGACAGTGGA
  GCAGAAAATGAAGGTAGTTGTCACAGTGATCAGATGAGCAACGATTTCTCCAATGATGATGGTGTTGATG
  AAGGAATCTGTCTTGAAACCAATAGTGGAACTGAAAAGATCTCAAAATCTGGACTTGAAAAGAATTCCTT
  GATCTATGAACTTTTCTCTGTTATGGTTCATTCTGGGAGCGCTGCTGGTGGTCATTATTATGCATGTATA
  AAGTCATTCAGTGATGAGCAGTGGTACAGCTTCAATGATCAACATGTCAGCAGGATAACACAAGAGGACA
  TTAAGAAAACACATGGTGGATCTTCAGGAAGCAGAGGATATTATTCTAGTGCTTTCGCAAGTTCCACAAA
  TGCATATATGCTGATCTATAGACTGAAGGATCCAGCCAGAAATGCAAAATTTCTAGAAGTGGATGAATAC
  CCAGAACATATTAAAAACTTGGTGCAGAAAGAGAGAGAGTTGGAAGAACAAGAAAAGAGACAACGAGAAA
  TTGAGCGCAATACATGCAAGATAAAATTATTCTGTTTGCATCCTACAAAACAAGTAATGATGGAAAATAA
  ATTGGAGGTTCATAAGGATAAGACATTAAAGGAAGCAGTAGAAATGGCTTATAAGATGATGGATTTAGAA
  GAGGTAATACCCCTGGATTGCTGTCGCCTTGTTAAATATGATGAGTTTCATGATTATCTAGAACGGTCAT
  ATGAAGGAGAAGAAGATACACCAATGGGGCTTCTACTAGGTGGCGTCAAGTCAACATATATGTTTGATCT
  GCTGTTGGAGACGAGAAAGCCTGATCAGGTTTTCCAATCTTATAAACCTGGAGAAGTGATGGTGAAAGTT
  CATGTTGTTGATCTAAAGGCAGAATCTGTAGCTGCTCCTATAACTGTTCGTGCTTACTTAAATCAGACAG
  TTACAGAATTCAAACAACTGATTTCAAAGGCCATCCATTTACCTGCTGAAACAATGAGAATAGTGCTGGA
  ACGCTGCTACAATGATTTGCGTCTTCTCAGTGTCTCCAGTAAAACCCTGAAAGCTGAAGGATTTTTTAGA
  AGTAACAAGGTGTTTGTTGAAAGCTCCGAGACTTTGGATTACCAGATGGCCTTTGCAGACTCTCATTTAT
  GGAAACTCCTGGATCGGCATGCAAATACAATCAGATTATTTGTTTTGCTACCTGAACAATCCCCAGTATC
  TTATTCCAAAAGGACAGCATACCAGAAAGCTGGAGGCGATTCTGGTAATGTGGATGATGACTGTGAAAGA
  GTCAAAGGACCTGTAGGAAGCCTAAAGTCTGTGGAAGCTATTCTAGAAGAAAGCACTGAAAAACTCAAAA
  GCTTGTCACTGCAGCAACAGCAGGATGGAGATAATGGGGACAGCAGCAAAAGTACTGAGACAAGTGACTT
  TGAAAACATCGAATCACCTCTCAATGAGAGGGACTCTTCAGCATCAGTGGATAATAGAGAACTTGAACAG
  CATATTCAGACTTCTGATCCAGAAAATTTTCAGTCTGAAGAACGATCAGACTCAGATGTGAATAATGACA
  GGAGTACAAGTTCAGTGGACAGTGATATTCTTAGCTCCAGTCATAGCAGTGATACTTTGTGCAATGCAGA
  CAATGCTCAGATCCCTTTGGCTAATGGACTTGACTCTCACAGTATCACAAGTAGTAGAAGAACGAAAGCA
  AATGAAGGGAAAAAAGAAACATGGGATACAGCAGAAGAAGACTCTGGAACTGATAGTGAATATGATGAGA
  GTGGCAAGAGTAGGGGAGAAATGCAGTACATGTATTTCAAAGCTGAACCTTATGCTGCAGATGAAGGTTC
  TGGGGAAGGACATAAATGGTTGATGGTGCATGTTGATAAAAGAATTACTCTGGCAGCTTTCAAACAACAT
  TTAGAGCCCTTTGTTGGAGTTTTGTCCTCTCACTTCAAGGTCTTTCGAGTGTATGCCAGCAATCAAGAGT
  TTGAGAGCGTCCGGCTGAATGAGACACTTTCATCATTTTCTGATGACAATAAGATTACAATTAGACTGGG
  GAGAGCACTTAAAAAAGGAGAATACAGAGTTAAAGTATACCAGCTTTTGGTCAATGAACAAGAGCCATGC
  AAGTTTCTGCTAGATGCTGTGTTTGCTAAAGGAATGACTGTACGGCAATCAAAAGAGGAATTAATTCCTC
  AGCTCAGGGAGCAATGTGGTTTAGAGCTCAGTATTGACAGGTTTCGTCTAAGGAAAAAAACATGGAAGAA
  TCCTGGCACTGTCTTTTTGGATTATCATATTTATGAAGAAGATATTAATATTTCCAGCAACTGGGAGGTT
  TTCCTTGAAGTTCTTGATGGGGTAGAGAAGATGAAGTCCATGTCACAGCTTGCAGTTTTGTCAAGACGGT
  GGAAGCCTTCAGAGATGAAGTTGGATCCCTTCCAGGAGGTTGTATTGGAAAGCAGTAGTGTGGACGAATT
  GCGAGAGAAGCTTAGTGAAATCAGTGGGATTCCTTTGGATGATATTGAATTTGCTAAGGGTAGAGGAACA
  TTTCCCTGTGATATTTCTGTCCTTGATATTCATCAGGATTTAGACTGGAATCCTAAAGTTTCTACCCTGA
  ATGTCTGGCCTCTTTATATCTGTGATGATGGTGCGGTCATATTTTATAGGGATAAAACAGAAGAATTAAT
  GGAATTGACAGATGAGCAAAGAAATGAACTGATGAAAAAAGAAAGCAGTCGACTCCAGAAGACTGGACAT
  CGTGTAACATACTCACCTCGTAAAGAGAAAGCACTAAAAATATATCTGGATGGAGCACCAAATAAAGATC
  TGACTCAAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214048 representing NM_017944
  Red=Cloning site Green=Tags(s)
  
  MVPGEENQLVPKEAPLDHTSDKSLLDANFEPGKKNFLHLTDKDGEQPQILLEDSSAGEDSVHDRFIGPLP
  REGSGGSTSDYVSQSYSYSSILNKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYKWEFEESEEDP
  VTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLIN
  ELYQGKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGSSQAFASVEEALHAFIQPEILDGPNQYFCERCK
  KKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMTFPEELDMSTFIDVEDEKSPQTESCTDSG
  AENEGSCHSDQMSNDFSNDDGVDEGICLETNSGTEKISKSGLEKNSLIYELFSVMVHSGSAAGGHYYACI
  KSFSDEQWYSFNDQHVSRITQEDIKKTHGGSSGSRGYYSSAFASSTNAYMLIYRLKDPARNAKFLEVDEY
  PEHIKNLVQKERELEEQEKRQREIERNTCKIKLFCLHPTKQVMMENKLEVHKDKTLKEAVEMAYKMMDLE
  EVIPLDCCRLVKYDEFHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLETRKPDQVFQSYKPGEVMVKV
  HVVDLKAESVAAPITVRAYLNQTVTEFKQLISKAIHLPAETMRIVLERCYNDLRLLSVSSKTLKAEGFFR
  SNKVFVESSETLDYQMAFADSHLWKLLDRHANTIRLFVLLPEQSPVSYSKRTAYQKAGGDSGNVDDDCER
  VKGPVGSLKSVEAILEESTEKLKSLSLQQQQDGDNGDSSKSTETSDFENIESPLNERDSSASVDNRELEQ
  HIQTSDPENFQSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADNAQIPLANGLDSHSITSSRRTKA
  NEGKKETWDTAEEDSGTDSEYDESGKSRGEMQYMYFKAEPYAADEGSGEGHKWLMVHVDKRITLAAFKQH
  LEPFVGVLSSHFKVFRVYASNQEFESVRLNETLSSFSDDNKITIRLGRALKKGEYRVKVYQLLVNEQEPC
  KFLLDAVFAKGMTVRQSKEELIPQLREQCGLELSIDRFRLRKKTWKNPGTVFLDYHIYEEDINISSNWEV
  FLEVLDGVEKMKSMSQLAVLSRRWKPSEMKLDPFQEVVLESSSVDELREKLSEISGIPLDDIEFAKGRGT
  FPCDISVLDIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDKTEELMELTDEQRNELMKKESSRLQKTGH
  RVTYSPRKEKALKIYLDGAPNKDLTQD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017944
• ORF Size: 3861 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017944.4
• RefSeq Size: 7777 bp
• RefSeq ORF: 3864 bp
• Locus ID: 55031
• UniProt ID: Q96K76
• Cytogenetics: 11p15.3
• Domains: UCH
• MW: 147 kDa
• Gene Summary: Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER). Acts as a regulator of cell growth and genome integrity. May also indirectly regulate CDC25A expression at a transcriptional level.[UniProtKB/Swiss-Prot Function]