ERC1 (NM_178040) Human Tagged ORF Clone

CAT#: RC213864

ERC1 (Myc-DDK-tagged)-Human ELKS/RAB6-interacting/CAST family member 1 (ERC1), transcript variant epsilon

Product Images

Circular map for RC213864

• 

  Western blot validation of overexpression lysate (Cat# LY406029) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213864 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ERC1 protein (Cat# TP313864). The protein was produced from HEK293T cells transfected with ERC1 cDNA clone (Cat# RC213864) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ERC1
• Synonyms: Cast2; ELKS; ERC-1; RAB6IP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213864 representing NM_178040
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATGGAAGTGCCCGCTCTGTTGGGAAGGTGGAGCCGAGCAGCCAGAGCCCTGGGCGTTCACCCAGGC
  TTCCACGTTCCCCTCGCTTGGGTCACCGTCGAACCAACAGTACGGGAGGGAGTTCGGGAAGCAGTGTTGG
  AGGTGGCAGTGGGAAAACCCTTTCAATGGAAAATATACAATCTTTAAATGCTGCCTATGCCACCTCTGGC
  CCTATGTATCTAAGTGACCATGAAAATGTGGGTTCAGAAACACCTAAAAGCACCATGACACTTGGCCGTT
  CTGGGGGACGTCTGCCTTACGGTGTTCGGATGACTGCTATGGGTAGTAGCCCCAATATAGCTAGCAGTGG
  GGTTGCTAGTGACACCATAGCATTTGGAGAGCATCACCTCCCTCCTGTGAGTATGGCATCCACTGTACCT
  CACTCCCTTCGTCAGGCGAGAGATAACACAATCATGGATCTGCAGACACAGCTGAAGGAAGTATTAAGAG
  AAAATGATCTCTTGCGGAAGGATGTGGAAGTAAAGGAGAGCAAATTGAGTTCTTCAATGAATAGCATCAA
  GACCTTCTGGAGCCCAGAGCTGAAGAAGGAACGAGCCCTGAGAAAAGATGAAGCTTCCAAAATCACCATT
  TGGAAGGAACAGTACAGAGTTGTACAGGAGGAAAACCAGCACATGCAGATGACAATCCAGGCTCTCCAGG
  ATGAATTGCGGATCCAGAGGGACCTGAATCAGCTGTTTCAGCAGGATAGTAGCAGCAGGACTGGCGAACC
  TTGTGTAGCAGAGCTGACAGAGGAGAACTTTCAGAGGCTTCATGCTGAGCATGAGCGGCAGGCCAAAGAG
  CTGTTTCTTCTTCGAAAGACATTGGAGGAAATGGAGCTGCGTATTGAGACTCAAAAGCAGACCCTAAATG
  CTCGGGATGAATCCATTAAGAAGCTTCTGGAAATGTTGCAGAGCAAAGGACTTTCTGCCAAGGCTACCGA
  GGAAGACCATGAGAGAACAAGACGACTGGCAGAGGCAGAGATGCACGTTCATCACCTAGAAAGCCTTTTG
  GAGCAGAAGGAAAAAGAGAACAGTATGTTGAGAGAGGAGATGCATCGAAGGTTTGAGAATGCTCCTGATT
  CTGCCAAAACAAAAGCTCTGCAAACTGTTATTGAGATGAAGGATTCAAAAATTTCCTCTATGGAGCGTGG
  GCTTCGAGACCTGGAAGAGGAAATTCAGATGCTGAAATCGAATGGTGCTTTGAGTACTGAGGAAAGGGAA
  GAAGAAATGAAGCAAATGGAAGTGTATCGGAGCCATTCTAAATTTATGAAAAATAAGGTAGAACAACTGA
  AGGAGGAACTAAGTTCGAAAGAGGCTCAATGGGAGGAGCTGAAAAAGAAAGCGGCTGGTCTTCAGGCTGA
  GATTGGCCAGGTGAAACAGGAGCTGTCCAGAAAGGACACAGAACTACTCGCCCTGCAGACAAAGCTAGAA
  ACACTCACAAACCAGTTCTCAGATAGTAAACAGCACATTGAAGTGTTGAAGGAGTCCTTGACTGCTAAGG
  AGCAGAGGGCTGCCATCCTGCAGACTGAGGTGGATGCTCTCCGATTGCGTTTGGAAGAGAAGGAAACCAT
  GTTGAATAAAAAGACAAAACAAATTCAGGATATGGCTGAAGAGAAGGGGACACAAGCTGGAGAGATACAT
  GACCTCAAGGACATGTTGGATGTGAAGGAGCGGAAGGTTAATGTTCTTCAGAAGAAGATTGAAAATCTTC
  AAGAGCAGCTTAGAGACAAGGAAAAGCAGATGAGCAGCTTGAAAGAACGGGTCAAATCCTTGCAGGCTGA
  CACCACCAACACTGACACTGCCTTGACAACTTTGGAGGAGGCCCTTGCAGAGAAAGAGCGGACAATTGAA
  CGCTTAAAGGAGCAGAGGGACAGAGATGAGCGAGAGAAGCAAGAGGAAATTGATAACTACAAAAAAGATC
  TTAAAGACTTGAAGGAAAAAGTCAGCCTGTTGCAAGGCGACCTTTCAGAGAAAGAGGCTTCACTTTTGGA
  TCTGAAAGAGCATGCTTCTTCTCTGGCATCCTCAGGACTGAAAAAGGACTCACGGCTTAAGACACTAGAG
  ATTGCTTTGGAGCAGAAGAAGGAGGAGTGTCTGAAAATGGAATCACAATTGAAAAAGGCACATGAGGCAG
  CATTGGAAGCCAGAGCCAGTCCAGAGATGAGTGACCGAATACAGCACTTGGAGAGAGAGATCACCAGGTA
  CAAAGATGAATCTAGCAAGGCCCAGGCAGAAGTTGATCGACTCTTAGAAATCTTGAAGGAGGTGGAAAAT
  GAGAAGAATGACAAAGATAAGAAGATAGCTGAGTTGGAAAGGCAAGTGAAAGACCAGAATAAGAAGGTAG
  CAAATCTGAAGCACAAGGAACAGGTGGAAAAAAAGAAGAGTGCACAAATGTTAGAGGAGGCGCGACGACG
  GGAGGACAATCTCAACGACAGCTCTCAGCAGCTACAGGACAGTCTCCGTAAGAAGGATGACAGGATTGAA
  GAGCTGGAAGAAGCACTAAGAGAAAGTGTACAGATAACTGCAGAGCGGGAAATGGTGCTAGCACAAGAGG
  AATCAGCCAGGACCAATGCTGAAAAACAGGTGGAGGAGTTACTGATGGCCATGGAGAAGGTAAAGCAGGA
  ACTAGAATCCATGAAAGCAAAGCTGTCCTCCACCCAGCAGTCTCTGGCAGAAAAGGAAACTCACTTGACT
  AATCTTCGGGCAGAGAGAAGGAAACACTTAGAGGAAGTTCTGGAGATGAAGCAAGAAGCTCTTCTGGCTG
  CCATTAGTGAAAAAGACGCCAATATAGCTCTCTTGGAGCTTTCGTCCTCTAAGAAGAAGACCCAAGAGGA
  AGTGGCTGCCCTGAAGCGGGAGAAGGATCGTCTGGTACAGCAGCTTAAGCAGCAGACGCAAAATCGAATG
  AAGCTAATGGCCGACAACTACGAGGATGACCACTTCAAATCCTCCCATTCCAATCAAACAAATCACAAGC
  CCTCCCCAGACCAGATCATCCAGCCCCTCTTAGAACTTGACCAAAATAGAAGTAAATTAAAGTTGTACAT
  TGGACACCTGACAACCCTCTGCCATGACCGAGACCCCCTGATCCTCCGTGGACTCACTCCACCAGCTTCC
  TATAACTTGGACGATGACCAGGCGGCTTGGGAGAATGAGCTGCAGAAGATGACCCGGGGGCAGCTTCAGG
  ATGAGTTAGAGAAAGGTGAACGGGACAATGCAGAACTGCAGGAGTTTGCCAACGCCATTCTTCAGCAGAT
  AGCAGACCATTGTCCCGACATCCTAGAGCAAGTGGTCAACGCCCTGGAAGAGTCCTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213864 representing NM_178040
  Red=Cloning site Green=Tags(s)
  
  MYGSARSVGKVEPSSQSPGRSPRLPRSPRLGHRRTNSTGGSSGSSVGGGSGKTLSMENIQSLNAAYATSG
  PMYLSDHENVGSETPKSTMTLGRSGGRLPYGVRMTAMGSSPNIASSGVASDTIAFGEHHLPPVSMASTVP
  HSLRQARDNTIMDLQTQLKEVLRENDLLRKDVEVKESKLSSSMNSIKTFWSPELKKERALRKDEASKITI
  WKEQYRVVQEENQHMQMTIQALQDELRIQRDLNQLFQQDSSSRTGEPCVAELTEENFQRLHAEHERQAKE
  LFLLRKTLEEMELRIETQKQTLNARDESIKKLLEMLQSKGLSAKATEEDHERTRRLAEAEMHVHHLESLL
  EQKEKENSMLREEMHRRFENAPDSAKTKALQTVIEMKDSKISSMERGLRDLEEEIQMLKSNGALSTEERE
  EEMKQMEVYRSHSKFMKNKVEQLKEELSSKEAQWEELKKKAAGLQAEIGQVKQELSRKDTELLALQTKLE
  TLTNQFSDSKQHIEVLKESLTAKEQRAAILQTEVDALRLRLEEKETMLNKKTKQIQDMAEEKGTQAGEIH
  DLKDMLDVKERKVNVLQKKIENLQEQLRDKEKQMSSLKERVKSLQADTTNTDTALTTLEEALAEKERTIE
  RLKEQRDRDEREKQEEIDNYKKDLKDLKEKVSLLQGDLSEKEASLLDLKEHASSLASSGLKKDSRLKTLE
  IALEQKKEECLKMESQLKKAHEAALEARASPEMSDRIQHLEREITRYKDESSKAQAEVDRLLEILKEVEN
  EKNDKDKKIAELERQVKDQNKKVANLKHKEQVEKKKSAQMLEEARRREDNLNDSSQQLQDSLRKKDDRIE
  ELEEALRESVQITAEREMVLAQEESARTNAEKQVEELLMAMEKVKQELESMKAKLSSTQQSLAEKETHLT
  NLRAERRKHLEEVLEMKQEALLAAISEKDANIALLELSSSKKKTQEEVAALKREKDRLVQQLKQQTQNRM
  KLMADNYEDDHFKSSHSNQTNHKPSPDQIIQPLLELDQNRSKLKLYIGHLTTLCHDRDPLILRGLTPPAS
  YNLDDDQAAWENELQKMTRGQLQDELEKGERDNAELQEFANAILQQIADHCPDILEQVVNALEESS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178040
• ORF Size: 3348 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178040.4
• RefSeq Size: 6893 bp
• RefSeq ORF: 3351 bp
• Locus ID: 23085
• UniProt ID: Q8IUD2
• Cytogenetics: 12p13.33
• Protein Families: Druggable Genome
• MW: 127.9 kDa
• Gene Summary: The protein encoded by this gene is a member of a family of RIM-binding proteins. RIMs are active zone proteins that regulate neurotransmitter release. This gene has been found fused to the receptor-type tyrosine kinase gene RET by gene rearrangement due to the translocation t(10;12)(q11;p13) in thyroid papillary carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]