ARHGAP4 (NM_001666) Human Tagged ORF Clone

CAT#: RC213860

ARHGAP4 (Myc-DDK-tagged)-Human Rho GTPase activating protein 4 (ARHGAP4), transcript variant 2

Product Images

Circular map for RC213860

• 

  Western blot validation of overexpression lysate (Cat# LY419816) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213860 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARHGAP4
• Synonyms: C1; p115; RGC1; RhoGAP4; SrGAP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213860 representing NM_001666
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCTCACGGGAAGCTGCGGCGGGAGCGGGGGCTGCAGGCTGAGTATGAGACGCAAGTCAAAGAGA
  TGCGCTGGCAGCTGAGCGAGCAGCTGCGCTGCCTGGAGCTGCAGGGCGAGCTGCGGCGGGAGTTGCTGCA
  GGAGCTGGCAGAGTTCATGCGGCGCCGCGCTGAGGTGGAGCTGGAATACTCCCGGGGCCTGGAAAAGCTG
  GCCGAGCGCTTCTCCAGCCGTGGAGGCCGCCTGGGGAGCAGCCGGGAGCACCAAAGCTTCCGGAAGGAGC
  CGTCCCTCCTGTCGCCCTTGCACTGCTGGGCGGTGCTGCTGCAGCACACGCGGCAGCAGAGCCGGGAGAG
  CGCGGCCCTGAGTGAGGTGCTGGCCGGGCCCCTGGCCCAGCGCCTGAGTCACATTGCAGAGGACGTGGGG
  CGCCTGGTCAAGAAGAGCAGGGATCTGGAGCAGCAGCTGCAGGATGAGCTCCTGGAGGTGGTCTCAGAGC
  TCCAGACGGCCAAGAAGACGTACCAGGCATATCACATGGAGAGCGTGAATGCCGAGGCCAAGCTCCGGGA
  GGCCGAGCGGCAGGAGGAGAAGCGGGCAGGCCGGAGTGTCCCCACCACCACCGCTGGTGCCACTGAGGCA
  GGGCCCCTCCGCAAGAGCTCCCTCAAGAAGGGAGGGAGGCTGGTGGAGAAGCGGCAGGCCAAGTTCATGG
  AGCACAAACTCAAGTGCACAAAGGCGCGCAACGAGTACCTGCTTAGCCTGGCTAGTGTCAACGCTGCTGT
  CAGTAACTACTACCTGCATGACGTCTTGGACCTCATGGACTGCTGTGACACAGGGTTCCACCTGGCCCTG
  GGGCAGGTGCTCCGGAGCTACACGGCCGCTGAGAGCCGCACCCAAGCCTCCCAAGTGCAGGGCCTGGGCA
  GCCTGGAAGAAGCTGTGGAGGCCCTGGATCCTCCAGGGGACAAAGCCAAGGTTCTCGAGGTGCATGCTAC
  CGTCTTCTGTCCCCCGCTGCGCTTTGACTACCACCCCCATGATGGGGATGAGGTGGCTGAGATCTGCGTT
  GAAATGGAGCTGCGGGACGAGATTCTGCCCAGAGCCCAGAACATCCAGAGCCGCCTGGACCGACAGACCA
  TTGAGACAGAGGAGGTGAACAAGACTCTGAAGGCGACACTGCAGGCCCTGCTGGAGGTGGTGGCCTCGGA
  TGACGGGGATGTGCTTGATTCCTTCCAGACCAGCCCCTCCACCGAGTCCCTCAAGTCCACCAGCTCAGAC
  CCAGGCAGCCGGCAGGCGGGCCGGAGGCGCGGCCAGCAGCAGGAGACCGAAACCTTCTACCTCACGAAGC
  TCCAGGAGTATCTGAGTGGACGGAGCATCCTCGCCAAGCTGCAGGCCAAGCACGAGAAGCTGCAGGAGGC
  CCTTCAGCGAGGTGACAAGGAGGAGCAGGAGGTGTCTTGGACCCAGTACACACAGAGAAAATTCCAGAAG
  AGCCGCCAGCCCCGCCCCAGCTCCCAGTATAACCAGAGACTCTTTGGGGGAGACATGGAGAAGTTTATCC
  AGAGCTCAGGCCAGCCTGTGCCCCTGGTGGTGGAGAGCTGCATTCGCTTCATCAACCTCAATGGCCTGCA
  GCATGAAGGCATCTTCCGGGTATCGGGTGCCCAGCTCCGGGTCTCAGAGATCCGTGATGCCTTCGAGAGA
  GGGGAGGACCCACTGGTGGAGGGCTGCACTGCCCATGACCTGGACTCGGTGGCCGGGGTGCTGAAGCTCT
  ACTTCCGGAGCCTGGAGCCCCCACTCTTCCCCCCAGACCTGTTCGGCGAGCTGCTGGCTTCTTCGGAGCT
  GGAGGACACAGCGGAGAGGGTGGAGCACGTGAGCCGCCTGCTGTGGCGGCTGCCCGCGCCGGTGCTGGTG
  GTTCTGCGCTACCTCTTCACCTTCCTCAACCACCTGGCCCAGTACAGCGATGAGAACATGATGGACCCCT
  ACAACCTGGCCGTGTGCTTCGGGCCCACGCTGCTACCGGTGCCCGCTGGGCAGGACCCGGTGGCGCTGCA
  GGGCCGGGTGAACCAGCTGGTGCAGACGCTCATAGTGCAGCCCGATCGGGTCTTCCCGCCCCTGACCTCG
  CTGCCTGGCCCCGTCTACGAGAAGTGCATGGCACCGCCTTCCGCCAGCTGCCTGGGGGACGCCCAGCTGG
  AGAGCCTGGGGGCGGACAATGATCCGGAGCTGGAAGCCGAGATGCCCGCACAGGAGGATGACCTGGAGGG
  GGTCGTGGAGGCTGTGGCCTGCTTTGCCTACACGGGCCGCACAGCCCAGGAGCTGAGCTTCCGGCGGGGG
  GACGTACTGCGGCTGCACGAGAGGGCCTCGAGCGACTGGTGGCGGGGGGAGCACAACGGCATGCGGGGCC
  TCATCCCCCACAAGTATATCACGCTGCCCGCCGGGACGGAGAAGCAGGTGGTGGGCGCAGGGCTGCAGAC
  TGCAGGGGAGTCTGGGAGCAGTCCCGAGGGCCTCCTGGCATCGGAGCTGGTCCACCGGCCAGAGCCATGC
  ACCTCACCTGAGGCCATGGGACCCTCTGGACACAGACGACGCTGCTTGGTCCCAGCCTCCCCAGAGCAAC
  ACGTGGAGGTGGATAAGGCTGTGGCACAGAACATGGACTCTGTGTTTAAGGAGCTCTTGGGAAAGACCTC
  TGTCCGCCAGGGCCTTGGGCCAGCATCTACCACCTCTCCCAGTCCTGGGCCCCGAAGCCCAAAGGCCCCG
  CCCAGCAGCCGCCTGGGCAGGAACAAAGGCTTCTCCCGGGGCCCTGGGGCCCCAGCCTCACCCTCAGCTT
  CCCACCCCCAGGGCCTAGACACGACCCCCAAGCCACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213860 representing NM_001666
  Red=Cloning site Green=Tags(s)
  
  MAAHGKLRRERGLQAEYETQVKEMRWQLSEQLRCLELQGELRRELLQELAEFMRRRAEVELEYSRGLEKL
  AERFSSRGGRLGSSREHQSFRKEPSLLSPLHCWAVLLQHTRQQSRESAALSEVLAGPLAQRLSHIAEDVG
  RLVKKSRDLEQQLQDELLEVVSELQTAKKTYQAYHMESVNAEAKLREAERQEEKRAGRSVPTTTAGATEA
  GPLRKSSLKKGGRLVEKRQAKFMEHKLKCTKARNEYLLSLASVNAAVSNYYLHDVLDLMDCCDTGFHLAL
  GQVLRSYTAAESRTQASQVQGLGSLEEAVEALDPPGDKAKVLEVHATVFCPPLRFDYHPHDGDEVAEICV
  EMELRDEILPRAQNIQSRLDRQTIETEEVNKTLKATLQALLEVVASDDGDVLDSFQTSPSTESLKSTSSD
  PGSRQAGRRRGQQQETETFYLTKLQEYLSGRSILAKLQAKHEKLQEALQRGDKEEQEVSWTQYTQRKFQK
  SRQPRPSSQYNQRLFGGDMEKFIQSSGQPVPLVVESCIRFINLNGLQHEGIFRVSGAQLRVSEIRDAFER
  GEDPLVEGCTAHDLDSVAGVLKLYFRSLEPPLFPPDLFGELLASSELEDTAERVEHVSRLLWRLPAPVLV
  VLRYLFTFLNHLAQYSDENMMDPYNLAVCFGPTLLPVPAGQDPVALQGRVNQLVQTLIVQPDRVFPPLTS
  LPGPVYEKCMAPPSASCLGDAQLESLGADNDPELEAEMPAQEDDLEGVVEAVACFAYTGRTAQELSFRRG
  DVLRLHERASSDWWRGEHNGMRGLIPHKYITLPAGTEKQVVGAGLQTAGESGSSPEGLLASELVHRPEPC
  TSPEAMGPSGHRRRCLVPASPEQHVEVDKAVAQNMDSVFKELLGKTSVRQGLGPASTTSPSPGPRSPKAP
  PSSRLGRNKGFSRGPGAPASPSASHPQGLDTTPKPH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001666
• ORF Size: 2838 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001666.2, NP_001657.2
• RefSeq Size: 3236 bp
• RefSeq ORF: 2841 bp
• Locus ID: 393
• UniProt ID: P98171
• Cytogenetics: Xq28
• Domains: RhoGAP, FCH, SH3
• MW: 104.8 kDa
• Gene Summary: This gene encodes a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins belonging to the RAS superfamily. The protein encoded by the orthologous gene in rat is localized to the Golgi complex and can redistribute to microtubules. The rat protein stimulates the activity of some Rho GTPases in vitro. Genomic deletions of this gene and a neighboring gene have been found in patients with nephrogenic diabetes insipidus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]