MCM7 (NM_005916) Human Tagged ORF Clone

CAT#: RC213809

MCM7 (Myc-DDK-tagged)-Human minichromosome maintenance complex component 7 (MCM7), transcript variant 1

Product Images

Circular map for RC213809

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  Western blot validation of overexpression lysate (Cat# LY401787) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213809 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MCM7 protein (Cat# TP313809). The protein was produced from HEK293T cells transfected with MCM7 cDNA clone (Cat# RC213809) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MCM7
• Synonyms: CDC47; MCM2; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213809 representing NM_005916
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACTGAAGGACTACGCGCTAGAGAAGGAAAAGGTTAAGAAGTTCTTACAAGAGTTCTACCAGGATG
  ATGAACTCGGGAAGAAGCAGTTCAAGTATGGGAACCAGTTGGTTCGGCTGGCTCATCGGGAACAGGTGGC
  TCTGTATGTGGACCTGGACGACGTAGCCGAGGATGACCCCGAGTTGGTGGACTCAATTTGTGAGAATGCC
  AGGCGCTACGCGAAGCTCTTTGCTGATGCCGTACAAGAGCTGCTGCCTCAGTACAAGGAGAGGGAAGTGG
  TAAATAAAGATGTCCTGGACGTTTACATTGAGCATCGGCTAATGATGGAGCAGCGGAGTCGGGACCCTGG
  GATGGTCCGAAGCCCCCAGAACCAGTACCCTGCTGAACTCATGCGCAGATTTGAGCTGTATTTTCAAGGC
  CCTAGCAGCAACAAGCCTCGTGTGATCCGGGAAGTGCGGGCTGACTCTGTGGGGAAGTTGGTAACTGTGC
  GTGGAATCGTCACTCGTGTCTCTGAAGTCAAACCCAAGATGGTGGTGGCCACTTACACTTGTGACCAGTG
  TGGGGCAGAGACCTACCAGCCGATCCAGTCTCCCACTTTCATGCCTCTGATCATGTGCCCAAGCCAGGAG
  TGCCAAACCAACCGCTCAGGAGGGCGGCTGTATCTGCAGACACGGGGCTCCAGATTCATCAAATTCCAGG
  AGATGAAGATGCAAGAACATAGTGATCAGGTGCCTGTGGGAAATATCCCTCGTAGTATCACGGTGCTGGT
  AGAAGGAGAGAACACAAGGATTGCCCAGCCTGGAGACCACGTCAGCGTCACTGGTATTTTCTTGCCAATC
  CTGCGCACTGGGTTCCGACAGGTGGTACAGGGTTTACTCTCAGAAACCTACCTGGAAGCCCATCGGATTG
  TGAAGATGAACAAGAGTGAGGATGATGAGTCTGGGGCTGGAGAGCTCACCAGGGAGGAGCTGAGGCAAAT
  TGCAGAGGAGGATTTCTACGAAAAGCTGGCAGCTTCAATCGCCCCAGAAATATACGGGCATGAAGATGTG
  AAGAAGGCACTGCTGCTCCTGCTAGTCGGGGGTGTGGACCAGTCTCCTCGAGGCATGAAAATCCGGGGCA
  ACATCAACATCTGTCTGATGGGGGATCCTGGTGTGGCCAAGTCTCAGCTCCTGTCATACATTGATCGACT
  GGCGCCTCGCAGCCAGTACACAACAGGCCGGGGCTCCTCAGGAGTGGGGCTTACGGCAGCTGTGCTGAGA
  GACTCCGTGAGTGGAGAACTGACCTTAGAGGGTGGGGCCCTGGTGCTGGCTGACCAGGGTGTGTGCTGCA
  TTGATGAGTTCGACAAGATGGCTGAGGCCGACCGCACAGCCATCCACGAGGTCATGGAGCAGCAGACCAT
  CTCCATTGCCAAGGCCGGCATTCTCACCACACTCAATGCCCGCTGCTCCATCCTGGCTGCCGCCAACCCT
  GCCTACGGGCGCTACAACCCTCGCCGCAGCCTGGAGCAGAACATACAGCTACCTGCTGCACTGCTCTCCC
  GGTTTGACCTCCTCTGGCTGATTCAGGACCGGCCCGACCGAGACAATGACCTACGGTTGGCCCAGCACAT
  TACCTATGTGCACCAGCACAGCCGGCAGCCCCCCTCCCAGTTTGAACCTCTGGACATGAAGCTCATGAGG
  CGTTACATAGCCATGTGCCGCGAGAAGCAGCCCATGGTGCCAGAGTCTCTGGCTGACTACATCACAGCAG
  CATACGTGGAGATGAGGCGAGAGGCTTGGGCTAGTAAGGATGCCACCTATACTTCTGCCCGGACCCTGCT
  GGCTATCCTGCGCCTTTCCACTGCTCTGGCACGTCTGAGAATGGTGGATGTGGTGGAGAAAGAAGATGTG
  AATGAAGCCATCAGGCTAATGGAGATGTCAAAGGACTCTCTTCTAGGAGACAAGGGGCAGACAGCTAGGA
  CTCAGAGACCAGCAGATGTGATATTTGCCACCGTCCGTGAACTGGTCTCAGGGGGCCGAAGTGTCCGGTT
  CTCTGAGGCAGAGCAGCGCTGTGTATCTCGTGGCTTCACACCCGCCCAGTTCCAGGCGGCTCTGGATGAA
  TATGAGGAGCTCAATGTCTGGCAGGTCAATGCTTCCCGGACACGGATCACTTTTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213809 representing NM_005916
  Red=Cloning site Green=Tags(s)
  
  MALKDYALEKEKVKKFLQEFYQDDELGKKQFKYGNQLVRLAHREQVALYVDLDDVAEDDPELVDSICENA
  RRYAKLFADAVQELLPQYKEREVVNKDVLDVYIEHRLMMEQRSRDPGMVRSPQNQYPAELMRRFELYFQG
  PSSNKPRVIREVRADSVGKLVTVRGIVTRVSEVKPKMVVATYTCDQCGAETYQPIQSPTFMPLIMCPSQE
  CQTNRSGGRLYLQTRGSRFIKFQEMKMQEHSDQVPVGNIPRSITVLVEGENTRIAQPGDHVSVTGIFLPI
  LRTGFRQVVQGLLSETYLEAHRIVKMNKSEDDESGAGELTREELRQIAEEDFYEKLAASIAPEIYGHEDV
  KKALLLLLVGGVDQSPRGMKIRGNINICLMGDPGVAKSQLLSYIDRLAPRSQYTTGRGSSGVGLTAAVLR
  DSVSGELTLEGGALVLADQGVCCIDEFDKMAEADRTAIHEVMEQQTISIAKAGILTTLNARCSILAAANP
  AYGRYNPRRSLEQNIQLPAALLSRFDLLWLIQDRPDRDNDLRLAQHITYVHQHSRQPPSQFEPLDMKLMR
  RYIAMCREKQPMVPESLADYITAAYVEMRREAWASKDATYTSARTLLAILRLSTALARLRMVDVVEKEDV
  NEAIRLMEMSKDSLLGDKGQTARTQRPADVIFATVRELVSGGRSVRFSEAEQRCVSRGFTPAQFQAALDE
  YEELNVWQVNASRTRITFV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005916
• ORF Size: 2157 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005916.5
• RefSeq Size: 2821 bp
• RefSeq ORF: 2160 bp
• Locus ID: 4176
• UniProt ID: P33993
• Cytogenetics: 7q22.1
• Domains: MCM, AAA
• Protein Families: Transcription Factors
• Protein Pathways: Cell cycle, DNA replication
• MW: 81.1 kDa
• Gene Summary: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]