Orexin Receptor 2 (HCRTR2) (NM_001526) Human Tagged ORF Clone

CAT#: RC213760

HCRTR2 (Myc-DDK-tagged)-Human hypocretin (orexin) receptor 2 (HCRTR2)

Product Images

Circular map for RC213760

• 

  Western blot validation of overexpression lysate (Cat# LY400585) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213760 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Orexin Receptor 2
• Synonyms: ORXR2; OX2R; OXR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213760 representing NM_001526
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGCACCAAATTGGAGGACTCCCCCCCTTGTCGCAACTGGTCATCTGCTTCGGAGCTGAATGAAA
  CTCAAGAGCCCTTTTTAAACCCCACCGACTATGACGACGAGGAATTCCTGCGGTACCTGTGGAGGGAATA
  CCTGCACCCGAAAGAATATGAGTGGGTCCTGATCGCCGGGTACATCATCGTGTTCGTCGTGGCTCTCATT
  GGGAACGTCCTGGTTTGTGTGGCAGTGTGGAAGAACCACCACATGAGGACGGTAACCAACTACTTCATAG
  TCAATCTTTCTCTGGCTGATGTGCTCGTGACCATCACCTGCCTTCCAGCCAAACTGGTCGTGGATATCAC
  TGAGACCTGGTTTTTTGGACAGTCCCTTTGCAAAGTGATTCCTTATCTACAGACCGTGTCGGTGTCTGTG
  TCTGTCCTCACACTGAGCTGTATCGCCTTGGATCGGTGGTATGCAATCTGTCACCCTTTGATGTTTAAGA
  GCACAGCAAAGCGGGCCCGTAACAGCATTGTCATCATCTGGATTGTCTCCTGCATTATAATGATTCCTCA
  GGCCATCGTCATGGAGTGCAGCACCGTGTTCCCAGGCTTAGCCAATAAAACCACCCTCTTTACGGTGTGT
  GATGAGCGCTGGGGTGGTGAAATTTATCCCAAGATGTACCACATCTGTTTCTTTCTGGTGACATACATGG
  CACCACTGTGTCTCATGGTGTTGGCTTATCTGCAAATATTTCGCAAACTCTGGTGTCGACAGATCCCTGG
  AACATCATCTGTAGTTCAGAGAAAATGGAAGCCCCTGCAGCCTGTTTCACAGCCTCGAGGGCCAGGACAG
  CCAACGAAGTCCCGGATGAGCGCTGTGGCGGCTGAAATAAAGCAGATCCGAGCCAGAAGGAAAACAGCCC
  GGATGTTGATGATTGTGCTTTTGGTATTTGCAATTTGCTATCTACCAATTAGCATCCTCAATGTGCTAAA
  GAGAGTATTTGGGATGTTTGCCCATACTGAAGACAGAGAGACTGTGTATGCCTGGTTTACCTTTTCACAC
  TGGCTTGTATATGCCAATAGTGCTGCGAATCCAATTATTTATAATTTTCTCAGTGGAAAATTTCGAGAGG
  AATTTAAAGCTGCGTTTTCTTGCTGTTGCCTTGGAGTTCACCATCGCCAGGAGGATCGGCTCACCAGGGG
  ACGAACTAGCACAGAGAGCCGGAAGTCCTTGACCACTCAAATCAGCAACTTTGATAACATATCAAAACTT
  TCTGAGCAAGTTGTGCTCACTAGCATAAGCACACTCCCAGCAGCCAATGGAGCAGGACCACTTCAAAACT
  GG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213760 representing NM_001526
  Red=Cloning site Green=Tags(s)
  
  MSGTKLEDSPPCRNWSSASELNETQEPFLNPTDYDDEEFLRYLWREYLHPKEYEWVLIAGYIIVFVVALI
  GNVLVCVAVWKNHHMRTVTNYFIVNLSLADVLVTITCLPAKLVVDITETWFFGQSLCKVIPYLQTVSVSV
  SVLTLSCIALDRWYAICHPLMFKSTAKRARNSIVIIWIVSCIIMIPQAIVMECSTVFPGLANKTTLFTVC
  DERWGGEIYPKMYHICFFLVTYMAPLCLMVLAYLQIFRKLWCRQIPGTSSVVQRKWKPLQPVSQPRGPGQ
  PTKSRMSAVAAEIKQIRARRKTARMLMIVLLVFAICYLPISILNVLKRVFGMFAHTEDRETVYAWFTFSH
  WLVYANSAANPIIYNFLSGKFREEFKAAFSCCCLGVHHRQEDRLTRGRTSTESRKSLTTQISNFDNISKL
  SEQVVLTSISTLPAANGAGPLQNW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001526
• ORF Size: 1332 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001526.5
• RefSeq Size: 1843 bp
• RefSeq ORF: 1335 bp
• Locus ID: 3062
• UniProt ID: O43614
• Cytogenetics: 6p12.1
• Protein Families: Druggable Genome
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 50.5 kDa
• Gene Summary: The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein binds the hypothalamic neuropeptides orexin A and orexin B. A related gene (HCRTR1) encodes a G-protein coupled receptor that selectively binds orexin A. [provided by RefSeq, Jan 2009]