Cordon bleu homolog (COBL) (NM_015198) Human Tagged ORF Clone

CAT#: RC213560

COBL (Myc-DDK-tagged)-Human cordon-bleu homolog (mouse) (COBL)

Product Images

Circular map for RC213560

• 

  Western blot validation of overexpression lysate (Cat# LY414720) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213560 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified COBL protein (Cat# TP313560). The protein was produced from HEK293T cells transfected with COBL cDNA clone (Cat# RC213560) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cordon bleu homolog
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213560 representing NM_015198
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCGCCGCGCGCCTCGGCGGCCAAGCCCCCGACCGGGAGGAAGATGAAGGCTCGTGCTCCCCCAC
  CTCCTGGAAAGGCTGCCACTCTGCATGTGCACAGTGACCAGAAGCCCCCCCACGATGGGGCCCTCGGGTC
  GCAGCAGAACTTGGTTCGCATGAAGGAGGCGCTGAGGGCCAGCACCATGGACGTCACCGTGGTCCTGCCT
  AGTGGGCTGGAGAAGAGGAGCGTGCTCAATGGGAGCCATGCGATGATGGACCTACTGGTTGAACTTTGCC
  TTCAGAACCACCTGAATCCATCCCACCATGCCCTTGAAATTCGGTCTTCAGAAACCCAACAACCTTTGAG
  TTTTAAGCCAAATACTTTGATTGGGACCCTGAATGTGCATACTGTGTTTCTGAAAGAAAAAGTTCCTGAA
  GAGAAGGTTAAGCCTGGTCCCCCTAAGGTGCCTGAGAAATCTGTGCGTTTGGTCGTGAATTACCTGCGGA
  CACAAAAAGCTGTTGTGCGTGTGAGCCCTGAGGTTCCTCTCCAGAATATTCTCCCAGTCATTTGTGCAAA
  GTGTGAGGTCAGCCCAGAGCACGTGGTTCTCCTCAGGGACAACATTGCCGGAGAGGAGCTGGAGCTGTCC
  AAGTCCCTGAACGAGCTCGGGATAAAGGAGCTCTACGCGTGGGACAACAGAAGAGAAACCTTTAGGAAAT
  CATCACTTGGCAATGATGAGACAGATAAAGAGAAGAAAAAATTTCTGGGATTTTTCAAAGTTAATAAAAG
  AAGCAATAGTAAGGGCTGTTTAACGACCCCCAACTCCCCATCCATGCACTCACGTTCTCTTACGCTGGGT
  CCATCCCTCTCGCTGGGCAGCATCTCAGGGGTGTCCGTGAAGTCGGAGATGAAGAAGCGCCGAGCCCCTC
  CTCCTCCAGGTTCAGGGCCACCTGTGCAAGACAAGGCATCGGAAAAGGTATCTCTTGGGTCACAGATTGA
  TTTACAGAAGAAGAAGCGGCGAGCGCCAGCTCCCCCTCCACCACAGCCACCACCACCGAGTCCCCTGATC
  CCCAACCGCACTGAGGATAAGGAGGAGAACAGGAAGAGCACGATGGTAAGCCTGCCCCTGGGGTCTGGCA
  GCCACTGCAGCCCGGATGGAGCCCCGCAGGTGCTGTCAGAGGCGGAGGAGACCGTGTCAGTTGGCAGCTG
  TTTTGCGTCGGAGGACACGACCGAGGACTCAGGAGTGATGAGTTCCCCCTCAGACATCGTCTCTCTGGAC
  TCGCAGCAGGACAGCATGAAATACAAAGACAAGTGGGCCACAGACCAGGAAGACTGCAGTGACCAGGACC
  TCGCTGGAACCCCAGACCTGGGTCCCCAGAAGAGCCCCTTGTGGGAGAAGAATGGCTCTGAGAACTCACA
  TCTGAGAACTGAAAAAGCTGTCACAGCCTCAAATGATGAAGAAGACCTATTAATTGCTGGAGAGTTCCGT
  AAAACCCTTGCAGAACTTGATGAAGACCTGGAAGAAATGGAAGACAGCTATGAAACAGACACCAGCTCCC
  TCACCAGCTCCATCCATGGTGCATCCAACCACTGCCCACAGGATGCCATGATCCCTCACGGCGACACAGA
  TGCAATCCCAGTAACATTCATAGGGGAAGTTTCAGATGATCCTGTGGATTCGGGGTTGTTTTCCAATAGA
  AACAACAATGCTGGGTCTTTCGACTCGGAGGGTGTTGCCAGCAGGAGAGACTCCCTGGCGCCACTTCAAG
  CTGAGCACAGCCAGCCCCATGAAAAGGCAAGGGAGGAAGTACCTGCCCTGCACCCCGCTTCCCATGACGT
  CGGAAAAGGAATCCGTGTGGCCTTATCTAACATCTCTAAAGATGGGAATCTAATGGAAACGGCGCCCAGG
  GTGACTTCTTTTGCTTCGAATCTTCACACAGACAACCTAAATGCAAAAGTGAAAGACAAAGTGTATGGCT
  GTGCTGACGGGGAGAGGACTCAAGCCACAGAGAGAGTGAATTCCCAACCGGTGAATGAAAAGGACAGCAA
  CGATAAAAACGCTGCCTTGGCACCAACATCATGGCACCAACGAGGCCAAAACCCAGGGAAAAGCTACAGA
  CTTAAGCACGGCCTCACAACGTATAAAATCATTCCTCCAAAGTCAGAGATGCGATGTTACGACAGAGATG
  TGTCCCTCTCCACCGGAGCCATTAAGATTGACGAGCTGGGGAACTTGGTGAGTCCTCACGCCACCGGCAT
  CAGGATCATTTCCCTGTCTTCGTCTGTGCCTGAAGCAGAATCTCAGCCCATTGGGAAAGTCAGAGAGTTC
  TGGAGGTGCAACTCTGTGGAAAAACACCTGGGCCGACCCTCAGAGAGCTCTGCCAGGGGACCCCCCTCCA
  CCCCTGTGCCCACGCAGACGCAGAATCCAGAGAGCAGACTCCAAGCAGACCCCAAGCCAATATCGCCCCA
  GCAGAAGTCTGCCCACCATGAGGGCCGGAACCCCCTAGGGGAGGGGAGAAACCAGCCCCCCACCATGGGC
  ATGGGTCACGTGAGGGTGCCAGCAGCTCACACCACAGAAGTCACATTTCTCAAGCCTCAGAGAAGAACGT
  CCAGCCAGTATGTGGCCTCTGCCATTGCCAAGCGCATAGGAGCCCCAAAAGTCCATGCTGATGTGGTGAG
  GCCACACGGTTATGCCGAGAAGGGTTATGCAGGCAAGGCGCCAGTGCTGGCTGCACCACCTGTCACTGTG
  AAAGATGACAGGACATCCAGCCCACACTCAGAGACACAAGGATGGAAGGATGGTGCCCAGTGGCCCTGTG
  TCACTCCTCCCAACAACCACGGGGAAGATTTGGCAGTGGGAGCCCCTCCTAGGGGGGAGGTCATTGGCCC
  ACACAGGAAGTTGTCTACTCAGGACAGACCTGCTGCTATCCACAGAAGCTCCTGTTTCTCACTGGTTCAG
  TCTTCCCAGAGGGATCGTGTTTCTGTGGGACAGAGCTGTGGTTTCAGTGGAAAGCAAAGCACAAGTAGCC
  AGGAGGCCAGCTCAGCATCTGAGCCCAGACGCGCACCTGATGGTACAGACCCACCCCCTCCACACACATC
  TGACACTCAGGCCTGCAGCAGGGAGCTGGTCAATGGCTCTGTGCGCGCCCCAGGCCACGGCGAGCCTTCC
  CACCCTCCAGGAGGGTCTGGCACAGAAAGCCACATTCTCCTAGAAAGAGAGGAAAAGCCCAGTGTGTTCT
  CTACAGATGGAAATGAAACAGACAGTATTTGGCCACCCAGCATTTTTGGGCCGAAGAAAAAATTCAAACC
  TGTTGTCCAGAGACCAGTCCCAAAAGACACATCCCTGCACTCTGCCCTGATGGAAGCCATCCACTCTGCG
  GGAGGGAAGGACAGACTACGCAAGACGGCAGAACACACCGGGGAGGGCAGGCCAGCGAAACTGTCCTACA
  CGGAGGCAGAGGGCGAACGATCTGCACTGCTGGCAGCTATCCGCGGGCACAGCGGCACCTGCAGCCTGAG
  GAAGGTGGCATCCTCTGCTTCTGAGGAGCTCCAGAGCTTCCGAGATGCCGCACTCTCTGCTCAGGGCTCG
  GAAAGTCCTCTGCTAGAAGACCTTGGTCTTCTGTCCCCACCAGCCATTCCCCCACCACCCCCTCCACCCT
  CCCAGGCTCTCTCTGCACCAAGGACGGCCTCCAGGTTCAGCACGGGCACCCTCAGCAACACCGCAGACGC
  AAGGCAAGCCTTGATGGACGCCATCCGCTCCGGCACAGGGGCTGCGAGACTGAGAAAGGTGCCCTTGCTC
  GTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213560 representing NM_015198
  Red=Cloning site Green=Tags(s)
  
  MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLP
  SGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPE
  EKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELS
  KSLNELGIKELYAWDNRRETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKGCLTTPNSPSMHSRSLTLG
  PSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLI
  PNRTEDKEENRKSTMVSLPLGSGSHCSPDGAPQVLSEAEETVSVGSCFASEDTTEDSGVMSSPSDIVSLD
  SQQDSMKYKDKWATDQEDCSDQDLAGTPDLGPQKSPLWEKNGSENSHLRTEKAVTASNDEEDLLIAGEFR
  KTLAELDEDLEEMEDSYETDTSSLTSSIHGASNHCPQDAMIPHGDTDAIPVTFIGEVSDDPVDSGLFSNR
  NNNAGSFDSEGVASRRDSLAPLQAEHSQPHEKAREEVPALHPASHDVGKGIRVALSNISKDGNLMETAPR
  VTSFASNLHTDNLNAKVKDKVYGCADGERTQATERVNSQPVNEKDSNDKNAALAPTSWHQRGQNPGKSYR
  LKHGLTTYKIIPPKSEMRCYDRDVSLSTGAIKIDELGNLVSPHATGIRIISLSSSVPEAESQPIGKVREF
  WRCNSVEKHLGRPSESSARGPPSTPVPTQTQNPESRLQADPKPISPQQKSAHHEGRNPLGEGRNQPPTMG
  MGHVRVPAAHTTEVTFLKPQRRTSSQYVASAIAKRIGAPKVHADVVRPHGYAEKGYAGKAPVLAAPPVTV
  KDDRTSSPHSETQGWKDGAQWPCVTPPNNHGEDLAVGAPPRGEVIGPHRKLSTQDRPAAIHRSSCFSLVQ
  SSQRDRVSVGQSCGFSGKQSTSSQEASSASEPRRAPDGTDPPPPHTSDTQACSRELVNGSVRAPGHGEPS
  HPPGGSGTESHILLEREEKPSVFSTDGNETDSIWPPSIFGPKKKFKPVVQRPVPKDTSLHSALMEAIHSA
  GGKDRLRKTAEHTGEGRPAKLSYTEAEGERSALLAAIRGHSGTCSLRKVASSASEELQSFRDAALSAQGS
  ESPLLEDLGLLSPPAIPPPPPPPSQALSAPRTASRFSTGTLSNTADARQALMDAIRSGTGAARLRKVPLL
  V
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015198
• ORF Size: 3783 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015198.5
• RefSeq Size: 5289 bp
• RefSeq ORF: 3786 bp
• Locus ID: 23242
• UniProt ID: O75128
• Cytogenetics: 7p12.1
• Domains: WH2
• MW: 135.4 kDa
• Gene Summary: This gene encodes a protein that contains WH2 domains (WASP, Wiskott-Aldrich syndrome protein, homology domain-2) that interact with actin. The encoded actin regulator protein is required for growth and assembly of brush border microvilli that play a role in maintaining intestinal homeostasis. A similar protein in mouse functions in midbrain neural tube closure. A pseudogene of this gene is located on chromosome X. [provided by RefSeq, Oct 2016]