PP11 (ENDOU) (NM_006025) Human Tagged ORF Clone

CAT#: RC213481

ENDOU (Myc-DDK-tagged)-Human endonuclease, polyU-specific (ENDOU), transcript variant 2

Product Images

Circular map for RC213481

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PP11
• Synonyms: P11; PP11; PRSS26
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213481 representing NM_006025
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGCCTGCATCTCCCTGGTATTGGCCGTGCTGTGTGGCCTGGCCTGGGCTGAGGACCACAAAGAGT
  CAGAGCCATTGCCACAGCTGGAGGAAGAGACAGAAGAGGCCCTCGCCAGCAACTTGTACTCGGCACCCAC
  CTCCTGCCAGGGCCGCTGCTACGAAGCCTTTGACAAGCACCACCAATGTCACTGCAATGCCCGCTGCCAA
  GAGTTTGGGAACTGCTGCAAGGATTTTGAGAGCCTGTGTAGTGACCACGAGGTCTCCCACAGCAGTGATG
  CCATAACAAAAGAGGAGATTCAGAGCATCTCTGAGAAGATCTACAGGGCAGACACCAACAAAGCCCAGAA
  GGAAGACATCGTTCTCAATAGCCAAAACTGCATCTCCCCGTCAGAGACCAGAAACCAAGTGGATCGCTGC
  CCAAAGCCACTCTTCACTTATGTCAATGAGAAGCTGTTCTCCAAGCCCACCTATGCAGCCTTCATCAACC
  TCCTCAACAACTACCAGCGGGCAACAGGCCATGGGGAGCACTTCAGTGCCCAGGAGCTGGCCGAGCAGGA
  CGCCTTCCTCAGAGAGATCATGAAGACAGCAGTCATGAAGGAGCTCTACAGCTTCCTCCATCACCAGAAT
  CGCTATGGCTCAGAGCAAGAGTTTGTCGATGACTTGAAGAACATGTGGTTTGGGCTCTATTCAAGAGGCA
  ATGAAGAGGGGGACTCGAGTGGCTTTGAACATGTCTTCTCAGGTGAGGTAAAAAAAGGCAAGGTTACTGG
  CTTCCATAACTGGATCCGCTTCTACCTGGAGGAGAAGGAGGGTCTGGTTGACTATTACAGTCACATCTAC
  GATGGGCCTTGGGATTCTTACCCCGATGTGCTGGCAATGCAGTTCAACTGGGACGGCTACTATAAGGAAG
  TGGGCTCTGCTTTCATCGGCAGCAGCCCTGAGTTTGAGTTTGCACTCTACTCCCTGTGCTTCATCGCCAG
  GCCAGGCAAAGTGTGCCAGTTAAGCCTGGGAGGATATCCCTTAGCTGTCCGGACATATACCTGGGACAAG
  TCCACCTATGGGAATGGCAAGAAGTACATCGCCACAGCCTACATAGTGTCTTCCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213481 representing NM_006025
  Red=Cloning site Green=Tags(s)
  
  MRACISLVLAVLCGLAWAEDHKESEPLPQLEEETEEALASNLYSAPTSCQGRCYEAFDKHHQCHCNARCQ
  EFGNCCKDFESLCSDHEVSHSSDAITKEEIQSISEKIYRADTNKAQKEDIVLNSQNCISPSETRNQVDRC
  PKPLFTYVNEKLFSKPTYAAFINLLNNYQRATGHGEHFSAQELAEQDAFLREIMKTAVMKELYSFLHHQN
  RYGSEQEFVDDLKNMWFGLYSRGNEEGDSSGFEHVFSGEVKKGKVTGFHNWIRFYLEEKEGLVDYYSHIY
  DGPWDSYPDVLAMQFNWDGYYKEVGSAFIGSSPEFEFALYSLCFIARPGKVCQLSLGGYPLAVRTYTWDK
  STYGNGKKYIATAYIVSST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006025
• ORF Size: 1107 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006025.4
• RefSeq Size: 2351 bp
• RefSeq ORF: 1110 bp
• Locus ID: 8909
• UniProt ID: P21128
• Cytogenetics: 12q13.11
• Domains: SO
• Protein Families: Protease
• MW: 42.1 kDa
• Gene Summary: This gene encodes a protein with endoribonuclease activity that binds polyuridine-enriched single-stranded RNA. This gene was initially characterized based on its high expression in placenta but was mischaracterized as a serine protease. In mouse, this gene promotes tolerance to self-antigens by regulating B cell activation-induced cell death (AICD). The protein may be useful as a tumor marker. Multiple alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq, Jul 2020]