GALR1 (NM_001480) Human Tagged ORF Clone

CAT#: RC213471

GALR1 (Myc-DDK-tagged)-Human galanin receptor 1 (GALR1)

Product Images

Circular map for RC213471

• 

  Western blot validation of overexpression lysate (Cat# LY419921) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213471 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GALR1
• Synonyms: GALNR; GALNR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213471 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGGCGGTCGGGAACCTCAGCGAGGGCAACGCGAGCTGGCCGGAGCCCCCCGCCCCGGAGCCCG
  GGCCGCTGTTCGGCATCGGCGTGGAGAACTTCGTCACGCTGGTGGTGTTCGGCCTGATCTTCGCGCTGGG
  CGTGCTGGGCAACAGCCTAGTGATCACCGTGCTGGCGCGCAGCAAGCCGGGCAAGCCGCGGAGCACCACC
  AACCTGTTCATCCTCAACCTGAGCATCGCCGACCTGGCCTACCTGCTCTTCTGCATCCCCTTCCAGGCCA
  CCGTGTACGCGCTGCCCACCTGGGTGCTGGGCGCCTTCATCTGCAAGTTCATCCACTACTTCTTCACCGT
  GTCCATGCTGGTGAGCATCTTCACCCTGGCCGCGATGTCCGTGGACCGCTACGTGGCCATCGTGCACTCG
  CGGCGCTCCTCCTCCCTCAGGGTGTCCCGCAACGCGCTGCTGGGCGTGGGCTGCATCTGGGCGCTGTCCA
  TTGCCATGGCCTCGCCCGTGGCCTACCACCAGGGCCTCTTCCACCCGCGCGCCAGCAACCAGACCTTCTG
  CTGGGAGCAGTGGCCCGACCCTCGCCACAAGAAGGCCTACGTGGTGTGCACCTTCGTCTTCGGCTACCTG
  CTGCCGCTCCTGCTCATCTGCTTCTGCTATGCCAAGGTCCTTAATCACTTGCATAAAAAGTTGAAGAACA
  TGTCAAAGAAGTCTGAAGCATCCAAGAAAAAGACTGCACAGACAGTTCTGGTGGTGGTTGTGGTGTTTGG
  AATCTCCTGGCTGCCGCACCACATCATCCATCTCTGGGCTGAGTTTGGAGTTTTCCCGCTGACGCCGGCT
  TCCTTCCTCTTCAGAATCACCGCCCACTGCCTGGCGTACAGCAATTCCTCCGTGAATCCTATCATTTATG
  CATTTCTCTCTGAAAATTTCAGGAAGGCCTATAAACAAGTGTTCAAGTGTCACATTCGCAAAGATTCACA
  CCTGAGTGATACTAAAGAAAATAAAAGTCGAATAGACACCCCACCATCAACCAATTGTACTCATGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213471 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MELAVGNLSEGNASWPEPPAPEPGPLFGIGVENFVTLVVFGLIFALGVLGNSLVITVLARSKPGKPRSTT
  NLFILNLSIADLAYLLFCIPFQATVYALPTWVLGAFICKFIHYFFTVSMLVSIFTLAAMSVDRYVAIVHS
  RRSSSLRVSRNALLGVGCIWALSIAMASPVAYHQGLFHPRASNQTFCWEQWPDPRHKKAYVVCTFVFGYL
  LPLLLICFCYAKVLNHLHKKLKNMSKKSEASKKKTAQTVLVVVVVFGISWLPHHIIHLWAEFGVFPLTPA
  SFLFRITAHCLAYSNSSVNPIIYAFLSENFRKAYKQVFKCHIRKDSHLSDTKENKSRIDTPPSTNCTHV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001480
• ORF Size: 1047 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001480.2, NP_001471.1
• RefSeq Size: 2787 bp
• RefSeq ORF: 1050 bp
• Locus ID: 2587
• UniProt ID: P47211
• Cytogenetics: 18q23
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 39 kDa
• Gene Summary: The neuropeptide galanin elicits a range of biological effects by interaction with specific G-protein-coupled receptors. Galanin receptors are seven-transmembrane proteins shown to activate a variety of intracellular second-messenger pathways. GALR1 inhibits adenylyl cyclase via a G protein of the Gi/Go family. GALR1 is widely expressed in the brain and spinal cord, as well as in peripheral sites such as the small intestine and heart. [provided by RefSeq, Jul 2008]