CEACAM20 (NM_001102597) Human Tagged ORF Clone

CAT#: RC213248

CEACAM20 (Myc-DDK-tagged)-Human carcinoembryonic antigen-related cell adhesion molecule 20 (CEACAM20), transcript variant 5L

Product Images

Circular map for RC213248

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  Western blot validation of overexpression lysate (Cat# LY420166) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213248 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CEACAM20 protein (Cat# TP313248). The protein was produced from HEK293T cells transfected with CEACAM20 cDNA clone (Cat# RC213248) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CEACAM20
• Synonyms: UNQ9366
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213248 representing NM_001102597
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCTGCTGACTCATGGGGACACCACTGGATGGGAATCCTGCTTTCAGCCTCGCTTTGTACCGTAT
  GGAGTCCTCCAGCTGCAGCCCAGCTCACCCTCAATGCCAACCCACTTGATGCCACCCAAAGTGAGGATGT
  TGTTCTGCCTGTGTTTGGGACCCCCAGGACACCCCAGATTCATGGCAGATCCAGAGAGCTGGCCAAACCC
  TCCATTGCAGTCAGCCCAGGCACTGCCATAGAGCAGAAGGACATGGTGACCTTCTACTGCACCACTAAGG
  ACGTCAACATTACCATCCACTGGGTTTCCAACAACCTCTCCGTTGTGTTCCATGAGCGCATGCAGCTGTC
  CAAGGATGGCAAGATCCTCACCATTCTCATTGTCCAGCGGGAGGACTCAGGGACTTACCAATGTGAAGCT
  CGAGATGCCCTTCTGAGCCAGAGGAGCGACCCCATCTTCCTGGATGTGAAGTATGGTCCTGATCCTGTTG
  AAATCAAATTGGAGTCTGGTGTTGCCAGTGGGGAGGTGGTTGAGGTGATGGAGGGCTCCAGCATGACCTT
  CTTAGCGGAAACAAAGTCTCACCCACCCTGTGCCTATACTTGGTTTCTCCTTGACTCCATTCTGTCTCAC
  ACCACGAGAACATTCACCATCCATGCTGTGTCCAGAGAACATGAGGGCCTGTACAGGTGCTTGGTGTCCA
  ACAGTGCCACCCACCTGTCCAGCCTGGGTACTCTGAAGGTCCGAGTACTTGAAACACTGACCATGCCTCA
  AGTCGTGCCTTCAAGCCTGAACCTTGTGGAGAATGCTAGGTCTGTGGACCTGACCTGCCAAACCGTCAAT
  CAGAGTGTGAATGTCCAGTGGTTCCTAAGTGGCCAGCCCCTCCTGCCCAGTGAGCACCTGCAGCTGTCAG
  CTGACAACAGGACCCTAATCATCCATGGCCTCCAGCGGAATGACACGGGGCCCTATGCCTGTGAGGTCTG
  GAACTGGGGCAGCCGGGCCCGGAGTGAGCCCCTTGAGCTGACCATCAACTATGGTCCTGACCAAGTGCAC
  ATCACCAGGGAGTCGGCATCTGAGATGATCAGCACCATAGAGGCAGAGCTCAACTCCAGCCTGACCCTGC
  AGTGTTGGGCCGAGTCCAAGCCAGGTGCTGAGTATCGCTGGACTCTTGAACACTCCACCGGGGAGCACCT
  GGGTGAGCAGCTGATTATCAGGGCTCTGACCTGGGAACACGACGGGATCTACAACTGCACAGCCTCCAAC
  TCTCTCACTGGCCTGGCCCGCTCCACTTCAGTCCTGGTCAAGGTGGTAGGTCCCCAGTCCTCCTCCCTGT
  CCTCAGGGGCCATCGCTGGTATTGTCATCGGGATCCTGGCTGTCATTGCTGTGGCCTCAGAACTGGGCTA
  TTTTCTCTACATCAGAAATGCCAGACGGCCCTCAAGGAAAACAACAGAGGACCCCAGTCATGAGACCTCA
  CAACCCATCCCGAAGGAGGAGCACCCCACAGAGCCCAGTTCCGAAAGCCTGAGTCCTGAGTATTGCAATA
  TATCCCAGCTTCAGGGACGGATCAGAGTCGAACTGATGCAACCACCAGACCTTCCAGAGGAGACCTATGA
  GACGAAGCTGCCTTCAGCAAGCCGTAGAGGCAATTCTTTCAGCCCCTGGAAGCCACCACCCAAACCTCTG
  ATGCCCCCACTCAGATTGGTCTCCACTGTGCCAAAAAACATGGAGTCAATCTATGAGGAGCTTGTGAATC
  CAGAGCCCAACACTTACATCCAAATCAACCCCTCCGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213248 representing NM_001102597
  Red=Cloning site Green=Tags(s)
  
  MGPADSWGHHWMGILLSASLCTVWSPPAAAQLTLNANPLDATQSEDVVLPVFGTPRTPQIHGRSRELAKP
  SIAVSPGTAIEQKDMVTFYCTTKDVNITIHWVSNNLSVVFHERMQLSKDGKILTILIVQREDSGTYQCEA
  RDALLSQRSDPIFLDVKYGPDPVEIKLESGVASGEVVEVMEGSSMTFLAETKSHPPCAYTWFLLDSILSH
  TTRTFTIHAVSREHEGLYRCLVSNSATHLSSLGTLKVRVLETLTMPQVVPSSLNLVENARSVDLTCQTVN
  QSVNVQWFLSGQPLLPSEHLQLSADNRTLIIHGLQRNDTGPYACEVWNWGSRARSEPLELTINYGPDQVH
  ITRESASEMISTIEAELNSSLTLQCWAESKPGAEYRWTLEHSTGEHLGEQLIIRALTWEHDGIYNCTASN
  SLTGLARSTSVLVKVVGPQSSSLSSGAIAGIVIGILAVIAVASELGYFLYIRNARRPSRKTTEDPSHETS
  QPIPKEEHPTEPSSESLSPEYCNISQLQGRIRVELMQPPDLPEETYETKLPSASRRGNSFSPWKPPPKPL
  MPPLRLVSTVPKNMESIYEELVNPEPNTYIQINPSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001102597
• ORF Size: 1788 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001102597.1, NP_001096067.1
• RefSeq Size: 1809 bp
• RefSeq ORF: 1791 bp
• Locus ID: 125931
• UniProt ID: Q6UY09
• Cytogenetics: 19q13.31
• Protein Families: Transmembrane
• MW: 65.6 kDa
• Gene Summary: Together with the tyrosine-protein kinase SYK, enhances production of the cytokine CXCL8/IL-8 via the NFKB pathway and may thus have a role in the intestinal immune response.[UniProtKB/Swiss-Prot Function]