Isoleucyl tRNA synthetase (IARS) (NM_013417) Human Tagged ORF Clone

CAT#: RC213208

IARS (Myc-DDK-tagged)-Human isoleucyl-tRNA synthetase (IARS), transcript variant long

Product Images

Circular map for RC213208

• 

  Western blot validation of overexpression lysate (Cat# LY415595) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213208 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Isoleucyl tRNA synthetase
• Synonyms: GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213208 representing NM_013417
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCAACAAGTTCCAGAAAACATAAATTTTCCTGCTGAAGAAGAGAAAATCTTGGAGTTTTGGACTG
  AATTTAATTGTTTTCAGGAATGCTTAAAGCAATCAAAACATAAACCAAAATTTACCTTCTATGATGGTCC
  TCCTTTTGCAACTGGACTGCCTCACTATGGACATATACTTGCGGGTACAATTAAAGATATAGTTACAAGA
  TATGCTCACCAGAGTGGGTTTCATGTTGACAGAAGATTTGGATGGGATTGCCATGGCTTACCTGTGGAAT
  ATGAAATTGATAAGACACTGGGAATCAGAGGACCAGAGGATGTGGCCAAAATGGGGATTACAGAGTATAA
  CAATCAGTGCCGAGCAATTGTGATGAGATATTCTGCTGAGTGGAAGTCTACTGTTAGCAGACTTGGCCGA
  TGGATTGACTTTGACAATGACTATAAAACTCTGTATCCACAATTCATGGAATCAGTCTGGTGGGTCTTCA
  AACAACTCTATGATAAAGGCCTTGTTTATAGAGGTGTGAAAGTCATGCCCTTCTCTACGGCATGTAACAC
  TCCACTTTCCAACTTCGAGTCACACCAGAATTATAAGGATGTTCAAGATCCTTCAGTATTTGTAACTTTC
  CCTTTGGAAGAAGATGAAACTGTATCTTTAGTTGCTTGGACAACCACTCCCTGGACTCTACCTAGTAACC
  TTGCTGTGTGTGTTAATCCAGAAATGCAATATGTGAAAATTAAAGATGTTGCCAGAGGACGATTACTCAT
  TTTAATGGAAGCCAGATTGTCAGCCCTCTATAAATTGGAGAGTGACTATGAGATCCTTGAAAGATTTCCT
  GGTGCCTATCTTAAAGGCAAGAAGTACAGGCCCCTGTTTGACTATTTCCTGAAGTGTAAAGAGAATGGCG
  CTTTCACTGTGCTTGTTGACAACTATGTGAAGGAAGAAGAAGGCACAGGGGTTGTCCACCAAGCTCCTTA
  CTTCGGTGCTGAGGACTATCGGGTCTGTATGGACTTTAACATTATTCGGAAAGACTCACTCCCTGTTTGC
  CCTGTGGATGCTTCAGGCTGCTTCACAACGGAGGTGACAGATTTCGCAGGACAGTATGTGAAGGATGCTG
  ACAAAAGTATCATCAGGACTTTGAAGGAACAAGGCCGACTTCTGGTTGCCACCACCTTCACTCACAGCTA
  CCCTTTTTGCTGGAGATCAGACACTCCTCTAATTTACAAAGCAGTGCCCAGCTGGTTTGTGCGAGTGGAG
  AACATGGTGGACCAGCTCCTAAGGAACAATGACCTGTGCTACTGGGTCCCAGAGTTGGTACGAGAAAAAC
  GATTTGGAAATTGGCTGAAAGATGCACGTGACTGGACAATTTCCAGAAACAGATACTGGGGCACCCCCAT
  CCCACTGTGGGTCAGCGATGACTTTGAGGAGGTGGTATGCATTGGGTCAGTGGCGGAACTTGAAGAACTG
  TCAGGAGCAAAGATCTCAGATCTCCACAGAGAGAGTGTTGACCACCTGACCATTCCTTCACGCTGTGGGA
  AGGGATCCTTGCACCGCATCTCTGAAGTGTTTGACTGTTGGTTTGAGAGTGGCAGCATGCCCTATGCTCA
  GGTTCATTACCCGTTTGAAAACAAGAGGGAGTTTGAGGATGCTTTTCCTGCAGATTTCATTGCCGAGGGC
  ATCGACCAAACCAGAGGATGGTTTTATACCCTGCTGGTGCTGGCCACGGCCCTCTTTGGACAACCGCCTT
  TCAAGAACGTAATTGTGAATGGGCTTGTCCTGGCAAGTGATGGCCAAAAAATGAGCAAACGGAAAAAGAA
  TTATCCAGATCCAGTTTCCATCATCCAGAAGTATGGTGCTGATGCCCTCAGATTATATCTGATTAACTCC
  CCTGTGGTGAGAGCAGAAAACCTCCGCTTTAAAGAAGAGGGTGTGCGGGACGTCCTTAAGGATGTACTGC
  TCCCATGGTACAATGCCTATCGCTTCTTAATCCAGAACGTTCTGAGGCTCCAGAAGGAGGAAGAAATAGA
  ATTTCTCTACAATGAGAACACGGTTAGAGAAAGCCCCAACATTACAGACCGGTGGATCCTGTCCTTCATG
  CAGTCTCTCATTGGCTTCTTTGAGACTGAAATGGCAGCTTATAGGCTTTATACTGTGGTGCCTCGCCTGG
  TCAAGTTTGTAGATATTCTGACCAATTGGTATGTTAGAATGAACCGCAGAAGATTAAAGGGTGAAAATGG
  GATGGAGGATTGTGTCATGGCCCTAGAAACCTTGTTTAGTGTTCTGCTTTCTCTTTGCAGACTTATAGCT
  CCCTACACACCTTTTCTCACTGAATTGATGTACCAGAATCTAAAGGTGCTGATTGACCCTGTTTCTGTTC
  AGGACAAGGACACACTCAGCATTCACTACCTCATGCTGCCCCGTGTTCGAGAAGAATTGATTGACAAGAA
  AACAGAGAGTGCAGTATCTCAGATGCAGTCTGTGATTGAACTTGGAAGAGTGATCAGAGACCGAAAAACT
  ATTCCCATAAAGTATCCTTTGAAAGAAATTGTGGTTATCCATCAAGATCCAGAAGCTCTTAAAGATATCA
  AGTCTTTGGAGAAGTATATCATTGAGGAACTCAATGTTCGAAAAGTTACACTGTCTACAGATAAAAACAA
  GTATGGCATTCGGCTAAGGGCAGAACCAGATCACATGGTCCTGGGGAAGCGTCTGAAGGGAGCCTTTAAG
  GCAGTGATGACGTCCATCAAGCAGTTGAGCAGTGAGGAGCTGGAGCAGTTCCAGAAGACTGGGACCATTG
  TTGTGGAAGGCCATGAATTGCACGATGAAGACATCCGCCTCATGTACACCTTTGATCAGGCCACAGGTGG
  GACTGCGCAATTTGAAGCACACTCAGATGCTCAGGCTTTGGTCCTCTTAGATGTCACTCCTGACCAGTCA
  ATGGTAGATGAAGGAATGGCTCGGGAAGTCATCAATCGCATACAGAAACTTCGCAAAAAGTGCAATCTGG
  TTCCAACTGATGAAATCACAGTGTACTATAAAGCAAAGTCTGAAGGAACATATCTGAATAGTGTTATTGA
  AAGCCACACAGAGTTCATATTTACCACCATAAAGGCTCCCTTGAAACCATATCCAGTTTCTCCATCGGAT
  AAAGTCCTTATTCAAGAAAAAACACAGTTGAAGGGATCTGAACTGGAAATTACACTCACCAGAGGATCTT
  CCCTTCCTGGTCCTGCTTGTGCATATGTCAATCTTAACATTTGTGCAAATGGCAGTGAACAAGGTGGAGT
  ATTGCTCCTGGAAAATCCAAAAGGTGACAATAGGTTGGACCTTTTAAAGCTGAAGAGTGTTGTCACTAGC
  ATTTTTGGTGTGAAAAATACAGAGCTGGCTGTCTTCCATGATGAAACAGAAATACAAAACCAAACTGACT
  TACTGAGTCTTAGTGGAAAAACACTTTGTGTGACTGCAGGATCGGCTCCCTCTCTGATCAACAGTTCTAG
  TACTCTTCTTTGTCAGTATATCAACCTACAGCTCCTGAATGCAAAGCCACAAGAGTGTTTAATGGGGACA
  GTGGGCACTCTCCTGCTTGAAAACCCACTTGGGCAGAATGGACTCACCCACCAAGGTCTTCTGTATGAAG
  CAGCCAAGGTGTTTGGCCTTCGGAGCAGGAAGCTAAAGCTGTTTCTGAATGAGACCCAAACGCAGGAAAT
  TACAGAAGACATCCCCGTGAAGACTTTGAATATGAAGACTGTGTATGTTTCTGTGTTACCAACAACAGCA
  GACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213208 representing NM_013417
  Red=Cloning site Green=Tags(s)
  
  MLQQVPENINFPAEEEKILEFWTEFNCFQECLKQSKHKPKFTFYDGPPFATGLPHYGHILAGTIKDIVTR
  YAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGITEYNNQCRAIVMRYSAEWKSTVSRLGR
  WIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVFVTF
  PLEEDETVSLVAWTTTPWTLPSNLAVCVNPEMQYVKIKDVARGRLLILMEARLSALYKLESDYEILERFP
  GAYLKGKKYRPLFDYFLKCKENGAFTVLVDNYVKEEEGTGVVHQAPYFGAEDYRVCMDFNIIRKDSLPVC
  PVDASGCFTTEVTDFAGQYVKDADKSIIRTLKEQGRLLVATTFTHSYPFCWRSDTPLIYKAVPSWFVRVE
  NMVDQLLRNNDLCYWVPELVREKRFGNWLKDARDWTISRNRYWGTPIPLWVSDDFEEVVCIGSVAELEEL
  SGAKISDLHRESVDHLTIPSRCGKGSLHRISEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEG
  IDQTRGWFYTLLVLATALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADALRLYLINS
  PVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKEEEIEFLYNENTVRESPNITDRWILSFM
  QSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMEDCVMALETLFSVLLSLCRLIA
  PYTPFLTELMYQNLKVLIDPVSVQDKDTLSIHYLMLPRVREELIDKKTESAVSQMQSVIELGRVIRDRKT
  IPIKYPLKEIVVIHQDPEALKDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFK
  AVMTSIKQLSSEELEQFQKTGTIVVEGHELHDEDIRLMYTFDQATGGTAQFEAHSDAQALVLLDVTPDQS
  MVDEGMAREVINRIQKLRKKCNLVPTDEITVYYKAKSEGTYLNSVIESHTEFIFTTIKAPLKPYPVSPSD
  KVLIQEKTQLKGSELEITLTRGSSLPGPACAYVNLNICANGSEQGGVLLLENPKGDNRLDLLKLKSVVTS
  IFGVKNTELAVFHDETEIQNQTDLLSLSGKTLCVTAGSAPSLINSSSTLLCQYINLQLLNAKPQECLMGT
  VGTLLLENPLGQNGLTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEITEDIPVKTLNMKTVYVSVLPTTA
  DF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013417
• ORF Size: 3786 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013417.1, NP_038203.1
• RefSeq Size: 4508 bp
• RefSeq ORF: 3789 bp
• Locus ID: 3376
• UniProt ID: P41252
• Cytogenetics: 9q22.31
• Domains: tRNA-synt_1
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis
• MW: 144.3 kDa
• Gene Summary: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Alternatively spliced transcript variants have been found. [provided by RefSeq, Nov 2012]