ATP2B1 (NM_001001323) Human Tagged ORF Clone

CAT#: RC213177

ATP2B1 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant 1.

Product Images

Circular map for RC213177

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATP2B1
• Synonyms: PMCA1; PMCA1kb
• Vector: pCMV6-AC-Myc-DDK
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213177 representing NM_001001323.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGCGACATGGCAAACAACTCAGTTGCTTACAGTGGTGTGAAAAACTCTTTGAAGGAAGCTAATCAT
  GATGGAGACTTTGGAATTACGCTCGCAGAGCTGCGGGCTCTCATGGAGCTCAGGTCCACAGATGCATTA
  CGAAAAATACAGGAAAGCTATGGAGATGTCTATGGAATTTGCACCAAATTGAAAACATCTCCCAATGAA
  GGTTTAAGTGGAAACCCTGCAGATTTAGAAAGAAGAGAAGCAGTGTTTGGAAAGAATTTTATACCTCCT
  AAAAAGCCAAAAACCTTTCTTCAATTAGTATGGGAAGCATTACAAGATGTCACTTTAATTATATTAGAA
  ATTGCAGCCATAGTATCATTGGGCCTTTCTTTTTATCAGCCTCCAGAAGGGGATAATGCACTTTGTGGA
  GAAGTTTCTGTTGGGGAGGAAGAAGGTGAAGGTGAAACTGGTTGGATTGAAGGAGCTGCAATCCTCTTG
  TCTGTAGTGTGTGTGGTGTTAGTAACAGCTTTCAATGACTGGAGTAAGGAAAAACAGTTTAGAGGTTTG
  CAGAGCCGAATTGAACAAGAACAGAAGTTCACTGTCATCAGGGGTGGTCAGGTCATTCAGATACCTGTA
  GCTGACATTACTGTTGGAGATATTGCTCAAGTGAAATATGGTGATCTTCTTCCAGCTGACGGCATACTT
  ATTCAAGGCAACGATCTTAAAATTGATGAAAGCTCATTGACTGGTGAATCAGATCATGTTAAAAAGTCT
  TTAGATAAGGATCCCTTACTTCTATCAGGTACTCATGTAATGGAAGGCTCTGGAAGAATGGTAGTTACA
  GCTGTAGGTGTAAATTCTCAAACTGGAATTATCTTTACCTTACTTGGAGCTGGAGGTGAAGAGGAAGAG
  AAGAAAGATGAGAAGAAAAAGGAAAAGAAAAATAAGAAACAAGATGGAGCTATTGAGAATCGCAACAAA
  GCAAAAGCCCAGGATGGTGCAGCCATGGAAATGCAGCCATTGAAGAGTGAAGAAGGTGGAGATGGTGAT
  GAAAAAGATAAAAAGAAAGCAAATTTGCCAAAAAAGGAAAAATCTGTTTTACAAGGGAAACTTACAAAA
  CTGGCTGTTCAGATTGGCAAAGCAGGTCTGTTGATGTCTGCCATCACAGTTATCATTCTAGTATTATAT
  TTTGTCATTGACACCTTCTGGGTTCAGAAAAGACCATGGCTTGCTGAGTGCACACCAATTTATATACAA
  TACTTTGTGAAGTTCTTCATTATTGGAGTTACAGTTTTAGTGGTCGCAGTGCCAGAAGGTCTTCCACTT
  GCAGTCACGATCTCACTGGCTTATTCAGTCAAAAAAATGATGAAAGATAATAACTTAGTAAGGCATCTG
  GATGCTTGTGAAACCATGGGAAATGCTACAGCTATTTGTTCAGATAAAACAGGAACTTTGACAATGAAC
  AGAATGACAGTCGTTCAAGCTTACATAAATGAAAAACATTATAAAAAGGTTCCTGAACCAGAAGCTATT
  CCACCAAATATTTTGTCCTATCTTGTAACAGGAATTTCTGTGAATTGTGCTTATACATCAAAAATATTG
  CCACCAGAGAAAGAGGGTGGATTACCTCGTCACGTTGGTAATAAAACTGAATGTGCCTTGTTGGGACTT
  CTTTTGGATTTAAAACGGGATTATCAGGATGTTAGAAATGAAATACCAGAAGAAGCACTGTACAAAGTC
  TACACCTTCAATTCTGTTAGGAAGTCCATGAGTACTGTCCTGAAAAATTCAGATGGAAGTTATCGAATA
  TTCAGCAAGGGTGCATCTGAGATAATTCTGAAAAAGTGTTTCAAAATCTTGAGTGCTAATGGTGAGGCA
  AAAGTATTCAGACCAAGGGACCGTGATGATATTGTAAAAACTGTGATTGAACCGATGGCATCAGAAGGC
  TTGAGAACCATATGTCTTGCATTCAGAGATTTTCCAGCAGGAGAACCAGAACCAGAGTGGGATAATGAA
  AATGATATTGTCACCGGCCTTACATGCATTGCTGTTGTGGGGATTGAAGATCCTGTGAGACCTGAGGTG
  CCAGATGCAATTAAAAAGTGTCAGAGGGCTGGAATTACTGTGCGGATGGTCACTGGTGATAATATTAAT
  ACTGCTCGGGCCATTGCTACCAAATGTGGTATTTTACATCCTGGGGAAGATTTTCTGTGCCTAGAAGGT
  AAAGATTTTAACAGAAGAATACGAAATGAAAAAGGAGAGATTGAGCAAGAGAGGATAGACAAGATTTGG
  CCAAAACTTCGAGTACTTGCAAGATCATCTCCTACTGATAAGCATACACTGGTTAAAGGTATAATTGAC
  AGCACTGTCTCAGACCAACGCCAGGTTGTAGCTGTAACTGGTGATGGTACAAATGATGGCCCAGCACTA
  AAGAAAGCAGATGTTGGATTTGCAATGGGTATTGCTGGAACTGATGTAGCTAAAGAAGCATCCGATATT
  ATTCTCACAGATGACAACTTTACAAGCATTGTTAAAGCAGTTATGTGGGGACGAAATGTCTATGACAGC
  ATCTCAAAATTCCTTCAGTTCCAACTTACTGTTAATGTAGTAGCAGTGATTGTTGCTTTTACGGGCGCC
  TGCATTACTCAAGACTCACCGCTTAAGGCTGTGCAGATGCTGTGGGTAAACCTCATAATGGATACACTC
  GCTTCCCTGGCTCTGGCAACGGAACCACCCACTGAGTCTCTCTTGCTTCGGAAACCTTATGGTAGAAAT
  AAGCCTCTCATCTCACGTACAATGATGAAGAATATTTTGGGTCATGCATTCTATCAACTTGTAGTAGTC
  TTTACACTCTTATTTGCTGGAGAAAAGTTTTTTGACATTGATAGTGGAAGAAATGCTCCTTTGCATGCT
  CCTCCTTCAGAACATTATACTATTGTTTTTAATACCTTTGTGCTGATGCAACTTTTCAACGAAATAAAT
  GCCCGGAAAATTCATGGTGAAAGAAATGTATTCGAAGGAATCTTTAACAATGCCATCTTCTGCACAATT
  GTTTTAGGCACTTTTGTGGTACAGATAATAATTGTGCAGTTTGGTGGAAAACCTTTCAGTTGTTCAGAA
  CTTTCAATAGAACAGTGGCTATGGTCAATATTCCTAGGAATGGGAACATTACTCTGGGGCCAGCTTATT
  TCAACAATTCCAACTAGCCGTTTAAAATTCCTCAAAGAAGCTGGTCATGGAACACAAAAGGAAGAAATA
  CCTGAGGAGGAATTAGCAGAGGATGTTGAAGAGATTGATCACGCTGAAAGGGAGTTGCGGCGTGGCCAA
  ATCTTGTGGTTTAGAGGTCTGAACAGAATCCAAACACAGATGGATGTAGTGAATGCTTTCCAGAGTGGA
  AGTTCCATTCAGGGGGCTCTAAGGCGGCAACCCTCCATCGCCAGCCAGCATCATGATGTAACAAATATT
  TCTACCCCTACACATGTAGTGTTTTCCTCTTCTACTGCTTCTACTACTGTGGGGTATTCGAGTGGTGAA
  TGCATTTCG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by RC213177
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MGDMANNSVAYSGVKNSLKEANHDGDFGITLAELRALMELRSTDALRKIQESYGDVYGICTKLKTSPNE
  GLSGNPADLERREAVFGKNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIVSLGLSFYQPPEGDNALCG
  EVSVGEEEGEGETGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVIRGGQVIQIPV
  ADITVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPLLLSGTHVMEGSGRMVVT
  AVGVNSQTGIIFTLLGAGGEEEEKKDEKKKEKKNKKQDGAIENRNKAKAQDGAAMEMQPLKSEEGGDGD
  EKDKKKANLPKKEKSVLQGKLTKLAVQIGKAGLLMSAITVIILVLYFVIDTFWVQKRPWLAECTPIYIQ
  YFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMN
  RMTVVQAYINEKHYKKVPEPEAIPPNILSYLVTGISVNCAYTSKILPPEKEGGLPRHVGNKTECALLGL
  LLDLKRDYQDVRNEIPEEALYKVYTFNSVRKSMSTVLKNSDGSYRIFSKGASEIILKKCFKILSANGEA
  KVFRPRDRDDIVKTVIEPMASEGLRTICLAFRDFPAGEPEPEWDNENDIVTGLTCIAVVGIEDPVRPEV
  PDAIKKCQRAGITVRMVTGDNINTARAIATKCGILHPGEDFLCLEGKDFNRRIRNEKGEIEQERIDKIW
  PKLRVLARSSPTDKHTLVKGIIDSTVSDQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDI
  ILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTL
  ASLALATEPPTESLLLRKPYGRNKPLISRTMMKNILGHAFYQLVVVFTLLFAGEKFFDIDSGRNAPLHA
  PPSEHYTIVFNTFVLMQLFNEINARKIHGERNVFEGIFNNAIFCTIVLGTFVVQIIIVQFGGKPFSCSE
  LSIEQWLWSIFLGMGTLLWGQLISTIPTSRLKFLKEAGHGTQKEEIPEEELAEDVEEIDHAERELRRGQ
  ILWFRGLNRIQTQMDVVNAFQSGSSIQGALRRQPSIASQHHDVTNISTPTHVVFSSSTASTTVGYSSGE
  CIS
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001323
• ORF Size: 3528 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001323.2
• RefSeq Size: 7121 bp
• RefSeq ORF: 3531 bp
• Locus ID: 490
• UniProt ID: P20020
• Cytogenetics: 12q21.33
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway
• MW: 129.5 kDa
• Gene Summary: The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 1. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]