OXCT2 (NM_022120) Human Tagged ORF Clone

CAT#: RC213077

OXCT2 (Myc-DDK-tagged)-Human 3-oxoacid CoA transferase 2 (OXCT2)

Product Images

Circular map for RC213077

• 

  Western blot validation of overexpression lysate (Cat# LY402907) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213077 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OXCT2
• Synonyms: FKSG25; SCOTT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213077 representing NM_022120
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCTGCGGCTCCTGGCGTCAGTGCTCGGGCGCGGGGTCCCCGCCGGCGGCTCAGGGCTCGCGC
  TGTCCCAGGGCTGCGCCCGCTGCTTTGCCACCAGTCCCCGGCTCCGTGCCAAGTTCTACGCGGACCCGGT
  GGAGATGGTGAAGGACATCTCTGACGGGGCGACCGTCATGATCGGGGGCTTCGGGCTCTGCGGGATCCCC
  GAGAACCTGATCGCCGCGCTGCTCAGGACCCGCGTGAAAGACCTGCAGGTGGTCAGCAGCAACGTGGGCG
  TGGAGGACTTCGGCCTGGGCCTCCTGCTGGCCGCCAGGCAGGTCCGTCGCATCGTCTGTTCCTACGTGGG
  CGAGAACACCCTGTGCGAGAGCCAGTACCTGGCAGGAGAGCTGGAGCTGGAGCTCACGCCCCAGGGCACC
  CTGGCCGAGCGCATCCGCGCGGGGGGCGCCGGGGTGCCCGCCTTCTACACCCCCACGGGCTACGGGACCC
  TGGTCCAGGAAGGGGGCGCCCCCATCCGCTACACCCCGGACGGCCACCTGGCGCTCATGAGCCAGCCCCG
  AGAGGTGAGGGAGTTCAACGGCGACCACTTCCTTTTGGAGCGCGCCATCCGGGCAGACTTCGCCCTGGTG
  AAAGGGTGGAAGGCCGACCGGGCAGGAAACGTGGTCTTCAGGAGAAGCGCCCGCAATTTCAACGTGCCCA
  TGTGCAAAGCTGCAGACGTCACGGCGGTGGAGGTGGAAGAGATCGTGGAGGTGGGGGCTTTCCCCCCAGA
  AGACATCCACGTTCCTAACATTTATGTAGATCGCGTGATAAAGGGGCAGAAATACGAGAAACGAATTGAG
  CGCTTAACGATCCTGAAAGAGGAAGATGGAGACGCTGGAAAGGAAGAGGACGCCAGGACGCGCATCATCA
  GACGCGCAGCTCTGGAATTTGAGGACGGCATGTACGCCAATCTGGGCATAGGCATCCCCCTGCTGGCCAG
  CAACTTCATCAGTCCCAGCATGACTGTACATCTTCACAGTGAGAACGGGATCCTGGGCCTGGGCCCGTTT
  CCCACGGAAGATGAGGTGGATGCCGACCTCATCAATGCAGGCAAGCAGACGGTCACGGTGCTTCCCGGGG
  GCTGCTTCTTCGCCAGCGACGACTCCTTCGCCATGATCCGAGGGGGACACATCCAACTAACCATGCTTGG
  AGCCATGCAGGTTTCCAAATACGGCGACCTGGCGAACTGGATGATCCCTGGCAAGAAGGTGAAAGGCATG
  GGCGGTGCCATGGACTTGGTGTCCAGTCAGAAGACCAGAGTGGTGGTCACCATGCAGCACTGCACAAAGG
  ACAACACCCCCAAGATCATGGAGAAATGCACCATGCCGCTGACCGGGAAGCGGTGCGTGGACCGCATCAT
  CACCGAGAAGGCCGTGTTTGACGTGCACAGGAAGAAAGAGCTGACGCTGAGGGAGCTCTGGGAGGGCCTG
  ACGGTGGACGACATCAAAAAGAGCACGGGGTGTGCCTTTGCTGTGTCCCCGAACCTCAGGCCCATGCAGC
  AGGTGGCACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213077 representing NM_022120
  Red=Cloning site Green=Tags(s)
  
  MAALRLLASVLGRGVPAGGSGLALSQGCARCFATSPRLRAKFYADPVEMVKDISDGATVMIGGFGLCGIP
  ENLIAALLRTRVKDLQVVSSNVGVEDFGLGLLLAARQVRRIVCSYVGENTLCESQYLAGELELELTPQGT
  LAERIRAGGAGVPAFYTPTGYGTLVQEGGAPIRYTPDGHLALMSQPREVREFNGDHFLLERAIRADFALV
  KGWKADRAGNVVFRRSARNFNVPMCKAADVTAVEVEEIVEVGAFPPEDIHVPNIYVDRVIKGQKYEKRIE
  RLTILKEEDGDAGKEEDARTRIIRRAALEFEDGMYANLGIGIPLLASNFISPSMTVHLHSENGILGLGPF
  PTEDEVDADLINAGKQTVTVLPGGCFFASDDSFAMIRGGHIQLTMLGAMQVSKYGDLANWMIPGKKVKGM
  GGAMDLVSSQKTRVVVTMQHCTKDNTPKIMEKCTMPLTGKRCVDRIITEKAVFDVHRKKELTLRELWEGL
  TVDDIKKSTGCAFAVSPNLRPMQQVAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022120
• ORF Size: 1551 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022120.1, NP_071403.1
• RefSeq Size: 1824 bp
• RefSeq ORF: 1554 bp
• Locus ID: 64064
• UniProt ID: Q9BYC2
• Cytogenetics: 1p34.2
• Protein Pathways: Butanoate metabolism, Synthesis and degradation of ketone bodies, Valine, leucine and isoleucine degradation
• MW: 56 kDa
• Gene Summary: The protein encoded by this gene catalyzes the transfer of a CoA group from succinate to acetoacetate and is an important enzyme in ketone body catabolism. The encoded protein localizes to the mitochondrion. This gene is intronless, and a pseudogene of this gene is located elsewhere on chromosome 1. [provided by RefSeq, Aug 2016]