ST8SIA5 (NM_013305) Human Tagged ORF Clone

CAT#: RC213009

ST8SIA5 (Myc-DDK-tagged)-Human ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5 (ST8SIA5)

Product Images

Circular map for RC213009

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ST8SIA5
• Synonyms: SIAT8-E; SIAT8E; ST8SiaV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213009 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTACGCGGACCCCTCGGCCAACCGGGATTTGTTGGGGAGCCGAACTTTGCTCTTCATCTTCATCT
  GCGCCTTTGCCTTGGTGACCTTGCTGCAACAGATCCTGTATGGCAGGAACTACATTAAGAGGTACTTTGA
  ATTTTATGAGGGGCCTTTTGAATATAACTCCACAAGATGCCTGGAGCTGAGGCACGAAATATTGGAAGTG
  AAGGTGCTGTCCATGGTGAAGCAGTCAGAGCTGTTCGACAGGTGGAAGAGCCTCCAGATGTGCAAATGGG
  CGATGAACATCTCTGAGGCCAACCAGTTCAAGTCTACTCTGTCCAGGTGCTGCAACGCCCCTGCCTTTCT
  CTTCACCACCCAGAAGAACACTCCCCTGGGGACAAAGCTCAAGTATGAGGTGGACACCAGTGGCATCTAC
  CACATCAACCAGGAGATCTTCCGCATGTTTCCCAAGGACATGCCCTACTACCGGTCCCAGTTTAAGAAGT
  GTGCTGTAGTGGGCAACGGAGGCATCTTGAAGAACAGCCGCTGCGGGAGGGAGATCAACAGCGCCGACTT
  CGTCTTCCGGTGCAACCTGCCCCCCATCTCAGAGAAGTACACCATGGATGTGGGGGTGAAGACGGATGTG
  GTCACTGTGAACCCCAGCATCATCACAGAGAGGTTCCACAAGCTGGAGAAGTGGCGGCGGCCGTTCTATC
  GCGTGCTGCAGGTGTACGAGAACGCGTCGGTGCTGCTGCCTGCCTTCTACAACACGCGCAACACCGACGT
  GTCCATCCGCGTCAAGTACGTGCTGGACGACTTCGAATCGCCGCAAGCTGTCTACTACTTCCATCCGCAG
  TACCTGGTCAACGTGTCGCGCTACTGGCTCAGCCTGGGGGTGCGCGCCAAGCGCATCAGCACCGGCCTCA
  TTCTGGTCACTGCGGCGCTGGAGCTCTGTGAGGAGGTGCACCTCTTTGGCTTCTGGGCCTTCCCCATGAA
  CCCCTCGGGCCTCTACATCACTCACCACTACTATGACAACGTCAAGCCGCGTCCCGGCTTCCACGCCATG
  CCCTCTGAGATCTTCAACTTCCTGCACTTGCACAGCCGAGGCATCCTCCGCGTGCACACGGGCACCTGCA
  GCTGCTGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213009 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRYADPSANRDLLGSRTLLFIFICAFALVTLLQQILYGRNYIKRYFEFYEGPFEYNSTRCLELRHEILEV
  KVLSMVKQSELFDRWKSLQMCKWAMNISEANQFKSTLSRCCNAPAFLFTTQKNTPLGTKLKYEVDTSGIY
  HINQEIFRMFPKDMPYYRSQFKKCAVVGNGGILKNSRCGREINSADFVFRCNLPPISEKYTMDVGVKTDV
  VTVNPSIITERFHKLEKWRRPFYRVLQVYENASVLLPAFYNTRNTDVSIRVKYVLDDFESPQAVYYFHPQ
  YLVNVSRYWLSLGVRAKRISTGLILVTAALELCEEVHLFGFWAFPMNPSGLYITHHYYDNVKPRPGFHAM
  PSEIFNFLHLHSRGILRVHTGTCSCC
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013305
• ORF Size: 1128 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013305.6
• RefSeq Size: 2627 bp
• RefSeq ORF: 1131 bp
• Locus ID: 29906
• UniProt ID: O15466
• Cytogenetics: 18q21.1
• Protein Families: Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - ganglio series, Metabolic pathways
• MW: 43.9 kDa
• Gene Summary: The protein encoded by this gene is a type II membrane protein that may be present in the Golgi apparatus. The encoded protein, which is a member of glycosyltransferase family 29, may be involved in the synthesis of gangliosides GD1c, GT1a, GQ1b, and GT3 from GD1a, GT1b, GM1b, and GD3, respectively. [provided by RefSeq, Jul 2008]