BDNF (NM_001709) Human Tagged ORF Clone
BDNF (Myc-DDK-tagged)-Human brain-derived neurotrophic factor (BDNF), transcript variant 4
|Product Name||BDNF (NM_001709) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC212882 representing NM_001709
Red=Cloning site Blue=ORF Green=Tags(s)
>RC212882 representing NM_001709
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||741 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_001709.1, NM_001709.2, NM_001709.3, NM_001709.4, NP_001700.2|
|RefSeq Size||3972 bp|
|RefSeq ORF||744 bp|
|Protein Families||Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Transmembrane|
|Protein Pathways||Huntington's disease, MAPK signaling pathway, Neurotrophin signaling pathway|
|Gene Summary||This gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders. [provided by RefSeq, Nov 2015]|
|The use of this cDNA Clones has been cited in the following citations:|
N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures
,Benicky, J;Sanda, M;Brnakova Kennedy, Z;Goldman, R;,
J. Biol. Chem. 2019
,PubMed ID 31558607
|cDNA Clone Resources|
|SC124461||BDNF (untagged)-Human brain-derived neurotrophic factor (BDNF), transcript variant 4||
|RG212882||BDNF (GFP-tagged) - Human brain-derived neurotrophic factor (BDNF), transcript variant 4||
|RC212882L1||Lenti ORF clone of Human brain-derived neurotrophic factor (BDNF), transcript variant 4, Myc-DDK-tagged||
|RC212882L2||Lenti ORF clone of Human brain-derived neurotrophic factor (BDNF), transcript variant 4, mGFP tagged||
|RC212882L3||Lenti ORF clone of Human brain-derived neurotrophic factor (BDNF), transcript variant 4, Myc-DDK-tagged||
|RC212882L4||Lenti ORF clone of Human brain-derived neurotrophic factor (BDNF), transcript variant 4, mGFP tagged||