PHLPP2 (NM_015020) Human Tagged ORF Clone

CAT#: RC212846

PHLPP2 (Myc-DDK-tagged)-Human PH domain and leucine rich repeat protein phosphatase 2 (PHLPP2)

Product Images

Circular map for RC212846

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PHLPP2 (Cat# RC212846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHLPP(Cat# TA590848). Positive lysates LY414856 (100ug) and LC414856 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY414856) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212846 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PHLPP2 protein (Cat# TP312846). The protein was produced from HEK293T cells transfected with PHLPP2 cDNA clone (Cat# RC212846) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PHLPP2
• Synonyms: PHLPPL; PPM3B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212846 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACGCAATGGGAGCAGAAATTGTTTGAATAGGAGAAGTAGGTTTGGTTCTCGAGAAAGAGACTGGC
  TAAGAGAAGATGTAAAGAGAGGCTGTGTTTACCTTTATGGAGCAGACACTACCACTGCCACTACAACCAC
  CACCACCTCCTCTTCCTCTTCCTCCTCCTCCTCTTCCTCTGACTTACATCTCGTCCTTTGCACTGTAGAG
  ACACCAGCATCAGAAATATGTGCTGGAGAGGGAAGAGAAAGTCTTTATTTACAGCTTCATGGAGACCTGG
  TCAGGAGACTGGAACCTACTGAACGACCTCTTCAGATCGTTTATGATTACTTATCCAGGCTGGGATTTGA
  TGATCCTGTGCGCATACAGGAGGAGGCTACAAATCCTGACCTCGGCTGTATGATTCGATTTTATGGTGAA
  AAACCATGCCACATGGATCGTTTGGATCGAATCCTATTGTCTGGCATCTATAATGTACGCAAGGGAAAGA
  CCCAGCTGCATAAGTGGGCTGAGCGCCTAGTTGTCCTCTGTGGTACCTGCCTTATCGTTTCCTCAGTGAA
  GGATTGTCAAACTGGAAAGATGCACATTTTGCCTCTGGTTGGTGGAAAGATAGAAGAAGTGAAGCGACGG
  CAATACTCCCTTGCTTTCAGCTCAGCAGGAGCCCAAGCTCAGACCTATCATGTCAGCTTCGAGACTTTGG
  CCGAGTACCAGCGATGGCAACGGCAAGCATCCAAGGTGGTGTCCCAGCGAATCAGTACCGTGGATCTCTC
  GTGTTACAGCCTCGAGGAGGTTCCTGAGCATCTCTTCTATAGTCAAGATATTACCTACCTCAACTTGCGA
  CACAACTTCATGCAGTTAGAAAGACCCGGAGGCCTCGATACACTCTACAAATTTTCTCAACTGAAGGGCC
  TGAACTTGTCCCATAATAAACTTGGGTTGTTTCCTATATTGTTATGCGAGATCTCTACCCTGACTGAGCT
  CAACCTTTCCTGTAATGGATTTCATGACCTACCAAGTCAAATTGGCAATCTGCTAAATCTTCAAACCCTC
  TGTCTTGATGGCAACTTTCTGACTACTTTACCTGAAGAATTGGGAAATCTACAACAGCTTTCCTCCTTGG
  GAATTTCCTTCAACAACTTTAGTCAAATTCCTGAGGTTTATGAGAAACTCACTATGTTAGATAGAGTGGT
  TATGGCAGGAAATTGCCTGGAAGTCCTGAACTTAGGGGTGCTGAATAGGATGAACCATATCAAGCATGTG
  GATTTAAGGATGAACCATTTGAAAACCATGGTTATTGAAAATCTGGAGGGAAATAAACACATCACCCACG
  TGGATCTGCGGGACAACCGACTGACTGACTTGGATCTTAGCTCCTTATGCAGCTTGGAACAGCTGCACTG
  TGGGCGGAATCAGCTGAGGGAGCTAACACTCAGTGGCTTTTCCCTTCGGACCCTCTATGCCAGTTCCAAC
  AGGCTGACAGCAGTGAACGTCTATCCAGTACCCAGCCTGCTCACTTTCTTGGATCTCTCCCGAAACCTGC
  TAGAGTGTGTCCCTGACTGGGCCTGTGAAGCAAAGAAGATAGAAGTATTAGATGTGAGCTATAATCTTCT
  CACAGAGGTTCCCGTGAGAATTCTGAGTAGCTTGAGTCTTAGAAAACTGATGCTGGGACACAATCATGTG
  CAAAACCTTCCAACACTGGTAGAGCACATCCCCCTCGAGGTGCTGGATCTTCAGCATAATGCACTCACGA
  GGCTGCCAGACACCCTCTTCTCCAAGGCCTTAAATCTCAGATACTTGAATGCATCTGCAAATAGTCTGGA
  GTCTTTACCATCCGCCTGCACTGGAGAGGAGAGTTTGAGTATGCTGCAGCTGCTTTATCTGACCAACAAT
  CTCCTGACGGATCAGTGCATACCTGTCCTGGTAGGGCACCTGCACCTGCGAATCTTGCACCTTGCAAACA
  ATCAGTTACAGACCTTTCCTGCAAGCAAACTAAATAAATTGGAGCAATTGGAGGAACTGAACCTAAGTGG
  CAACAAGCTTAAAACCATTCCCACAACCATAGCAAACTGTAAAAGGCTGCACACCCTTGTTGCACACTCC
  AACAACATCAGCATTTTCCCAGAAATACTGCAGTTGCCTCAGATCCAGTTTGTAGACCTAAGTTGCAACG
  ACTTGACAGAAATCCTGATTCCAGAGGCTTTGCCTGCTACATTACAAGACCTTGACCTGACTGGAAATAC
  AAATCTGGTTCTGGAACACAAGACACTGGACATATTTAGCCATATCACAACCCTGAAAATTGATCAGAAA
  CCTTTGCCAACCACAGATTCTACAGTTACGTCAACCTTCTGGAGCCATGGACTGGCTGAGATGGCAGGGC
  AGAGAAATAAGCTGTGTGTCTCAGCACTTGCTATGGATAGCTTTGCAGAGGGGGTGGGAGCTGTGTATGG
  CATGTTTGATGGAGACCGAAATGAGGAGCTCCCGCGCCTGCTGCAGTGTACGATGGCAGATGTGCTTTTA
  GAAGAGGTACAGCAGTCAACTAATGACACAGTTTTCATGGCTAACACCTTCTTGGTATCTCACAGGAAAT
  TAGGAATGGCTGGCCAGAAGTTGGGCTCCTCCGCTCTCCTGTGCTACATCCGCCCTGACACTGCCGATCC
  AGCAAGTAGCTTTAGCTTGACTGTAGCCAATGTTGGCACGTGCCAAGCAGTCCTGTGCCGAGGTGGGAAG
  CCAGTGCCCCTCTCTAAAGTCTTCAGCCTGGAGCAGGACCCAGAGGAGGCTCAAAGGGTGAAGGACCAAA
  AAGCCATCATCACAGAGGACAACAAAGTGAATGGGGTAACCTGCTGTACCCGGATGCTGGGCTGTACATA
  CCTCTACCCTTGGATCCTCCCCAAGCCCCACATATCTTCCACTCCGCTGACCATTCAAGATGAGTTGCTG
  ATTCTGGGAAACAAAGCATTGTGGGAACACTTGTCCTACACAGAAGCTGTCAATGCTGTACGTCACGTAC
  AAGACCCATTAGCAGCTGCTAAGAAGCTGTGCACATTAGCGCAGAGCTATGGCTGTCAGGACAATGTAGG
  GGCGATGGTAGTTTATTTGAATATTGGTGAGGAAGGCTGCACTTGTGAAATGAATGGGCTCACCCTCCCA
  GGTCCTGTGGGATTTGCTTCAACCACCACTATCAAGGATGCCCCTAAGCCAGCCACTCCATCCTCTAGCA
  GTGGGATTGCCTCTGAGTTCAGCAGTGAGATGTCCACCTCAGAGGTGAGCAGTGAAGTGGGGTCCACTGC
  TTCTGATGAGCATAATGCTGGGGGCCTGGACACTGCCTTGCTTCCGAGGCCAGAGCGGCGCTGCAGCCTC
  CACCCAACACCCACCTCTGGGCTGTTTCAGCGCCAGCCTTCTTCTGCTACCTTCTCCAGTAACCAGTCTG
  ACAACGGCCTGGACAGTGATGATGACCAGCCCGTTGAGGGGGTCATAACCAATGGCAGCAAGGTAGAGGT
  GGAAGTAGACATCCACTGCTGCAGGGGGAGGGATCTGGAGAACTCACCCCCTCTCATAGAGAGTTCTCCT
  ACCCTGTGTTCTGAGGAACATGCTAGAGGGTCGTGTTTTGGGATCCGAAGACAGAACAGTGTGAATAGTG
  GCATGCTCCTGCCAATGAGCAAGGACAGGATGGAGTTACAGAAGTCTCCCTCCACCTCCTGCCTCTATGG
  GAAGAAACTCTCCAATGGCTCTATTGTGCCCCTAGAGGACAGCCTGAACCTCATTGAAGTGGCCACAGAA
  GTGCCCAAGAGGAAAACTGGCTATTTTGCTGCCCCCACTCAGATGGAACCAGAGGACCAGTTTGTTGTGC
  CTCATGACCTGGAAGAAGAAGTGAAGGAACAAATGAAACAGCACCAGGACAGCCGGCTCGAGCCTGAGCC
  CCATGAAGAGGATCAGACCGAGCCCCCGGAGGAGTTCGACACAGCACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212846 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKRNGSRNCLNRRSRFGSRERDWLREDVKRGCVYLYGADTTTATTTTTTSSSSSSSSSSSDLHLVLCTVE
  TPASEICAGEGRESLYLQLHGDLVRRLEPTERPLQIVYDYLSRLGFDDPVRIQEEATNPDLGCMIRFYGE
  KPCHMDRLDRILLSGIYNVRKGKTQLHKWAERLVVLCGTCLIVSSVKDCQTGKMHILPLVGGKIEEVKRR
  QYSLAFSSAGAQAQTYHVSFETLAEYQRWQRQASKVVSQRISTVDLSCYSLEEVPEHLFYSQDITYLNLR
  HNFMQLERPGGLDTLYKFSQLKGLNLSHNKLGLFPILLCEISTLTELNLSCNGFHDLPSQIGNLLNLQTL
  CLDGNFLTTLPEELGNLQQLSSLGISFNNFSQIPEVYEKLTMLDRVVMAGNCLEVLNLGVLNRMNHIKHV
  DLRMNHLKTMVIENLEGNKHITHVDLRDNRLTDLDLSSLCSLEQLHCGRNQLRELTLSGFSLRTLYASSN
  RLTAVNVYPVPSLLTFLDLSRNLLECVPDWACEAKKIEVLDVSYNLLTEVPVRILSSLSLRKLMLGHNHV
  QNLPTLVEHIPLEVLDLQHNALTRLPDTLFSKALNLRYLNASANSLESLPSACTGEESLSMLQLLYLTNN
  LLTDQCIPVLVGHLHLRILHLANNQLQTFPASKLNKLEQLEELNLSGNKLKTIPTTIANCKRLHTLVAHS
  NNISIFPEILQLPQIQFVDLSCNDLTEILIPEALPATLQDLDLTGNTNLVLEHKTLDIFSHITTLKIDQK
  PLPTTDSTVTSTFWSHGLAEMAGQRNKLCVSALAMDSFAEGVGAVYGMFDGDRNEELPRLLQCTMADVLL
  EEVQQSTNDTVFMANTFLVSHRKLGMAGQKLGSSALLCYIRPDTADPASSFSLTVANVGTCQAVLCRGGK
  PVPLSKVFSLEQDPEEAQRVKDQKAIITEDNKVNGVTCCTRMLGCTYLYPWILPKPHISSTPLTIQDELL
  ILGNKALWEHLSYTEAVNAVRHVQDPLAAAKKLCTLAQSYGCQDNVGAMVVYLNIGEEGCTCEMNGLTLP
  GPVGFASTTTIKDAPKPATPSSSSGIASEFSSEMSTSEVSSEVGSTASDEHNAGGLDTALLPRPERRCSL
  HPTPTSGLFQRQPSSATFSSNQSDNGLDSDDDQPVEGVITNGSKVEVEVDIHCCRGRDLENSPPLIESSP
  TLCSEEHARGSCFGIRRQNSVNSGMLLPMSKDRMELQKSPSTSCLYGKKLSNGSIVPLEDSLNLIEVATE
  VPKRKTGYFAAPTQMEPEDQFVVPHDLEEEVKEQMKQHQDSRLEPEPHEEDQTEPPEEFDTAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015020
• ORF Size: 3969 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015020.3, NP_055835.2
• RefSeq Size: 8317 bp
• RefSeq ORF: 3972 bp
• Locus ID: 23035
• UniProt ID: Q6ZVD8
• Cytogenetics: 16q22.2
• MW: 146.7 kDa
• Gene Summary: Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis (PubMed:20513427). Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation (PubMed:21986499). Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity (PubMed:24530606).[UniProtKB/Swiss-Prot Function]