CORIN (NM_006587) Human Tagged ORF Clone

CAT#: RC212742

CORIN (Myc-DDK-tagged)-Human corin, serine peptidase (CORIN)

Product Images

Circular map for RC212742

• 

  Coomassie blue staining of purified CORIN protein (Cat# TP312742). The protein was produced from HEK293T cells transfected with CORIN cDNA clone (Cat# RC212742) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CORIN
• Synonyms: ATC2; CRN; Lrp4; PEE5; TMPRSS10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212742 representing NM_006587
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACAGTCTCCTGCCCTCGCTCCGGAAGAGCGCTGCCGCAGAGCCGGGTCCCCAAAGCCGGTCTTGA
  GAGCTGATGACAATAACATGGGCAATGGCTGCTCTCAGAAGCTGGCGACTGCTAACCTCCTCCGGTTCCT
  ATTGCTGGTCCTGATTCCATGTATCTGTGCTCTCGTTCTCTTGCTGGTGATCCTGCTTTCCTATGTTGGA
  ACATTACAAAAGGTCTATTTTAAATCAAATGGGAGTGAACCTTTGGTCACTGATGGTGAAATCCAAGGGT
  CCGATGTTATTCTTACAAATACAATTTATAACCAGAGCACTGTGGTGTCTACTGCACATCCCGACCAACA
  CGTTCCAGCCTGGACTACGGATGCTTCTCTCCCAGGGGACCAAAGTCACAGGAATACAAGTGCCTGTATG
  AACATCACCCACAGCCAGTGTCAGATGCTGCCCTACCACGCCACGCTGACACCTCTCCTCTCAGTTGTCA
  GAAACATGGAAATGGAAAAGTTCCTCAAGTTTTTCACATATCTCCATCGCCTCAGTTGCTATCAACATAT
  CATGCTGTTTGGCTGTACCCTCGCCTTCCCTGAGTGCATCATTGATGGCGATGACAGTCATGGACTCCTG
  CCCTGTAGGTCCTTCTGTGAGGCTGCAAAAGAAGGCTGTGAATCAGTCCTGGGGATGGTGAATTACTCCT
  GGCCGGATTTCCTCAGATGCTCCCAGTTTAGAAACCAAACTGAAAGCAGCAATGTCAGCAGAATTTGCTT
  CTCACCTCAGCAGGAAAACGGAAAGCAATTGCTCTGTGGAAGGGGTGAGAACTTTCTGTGTGCCAGTGGA
  ATCTGCATCCCCGGGAAACTGCAATGTAATGGCTACAACGACTGTGACGACTGGAGTGACGAGGCTCATT
  GCAACTGCAGCGAGAATCTGTTTCACTGTCACACAGGCAAGTGCCTTAATTACAGCCTTGTGTGTGATGG
  ATATGATGACTGTGGGGATTTGAGTGATGAGCAAAACTGTGATTGCAATCCCACAACAGAGCATCGCTGC
  GGGGACGGGCGCTGCATCGCCATGGAGTGGGTGTGTGATGGTGACCACGACTGTGTGGATAAGTCTGACG
  AGGTCAACTGCTCCTGTCACAGCCAGGGTCTGGTGGAATGCAGAAATGGACAATGTATCCCCAGCACGTT
  TCAATGTGATGGTGACGAGGACTGCAAGGATGGGAGTGATGAGGAGAACTGCAGCGTCATTCAGACTTCA
  TGTCAAGAAGGAGACCAAAGATGCCTCTACAATCCCTGCCTTGATTCATGTGGTGGTAGCTCTCTCTGTG
  ACCCGAACAACAGTCTGAATAACTGTAGTCAATGTGAACCAATTACATTGGAACTCTGCATGAATTTGCC
  CTACAACAGTACAAGTTATCCAAATTATTTTGGCCACAGGACTCAAAAGGAAGCATCCATCAGCTGGGAG
  TCTTCTCTTTTCCCTGCACTTGTTCAAACCAACTGTTATAAATACCTCATGTTCTTTTCTTGCACCATTT
  TGGTACCAAAATGTGATGTGAATACAGGCGAGCATATCCCTCCTTGCAGGGCATTGTGTGAACACTCTAA
  AGAACGCTGTGAGTCTGTTCTTGGGATTGTGGGCCTACAGTGGCCTGAAGACACAGATTGCAGTCAATTT
  CCAGAGGAAAATTCAGACAATCAAACCTGCCTGATGCCTGATGAATATGTGGAAGAATGCTCACCTAGTC
  ATTTCAAGTGCCGCTCAGGACAGTGTGTTCTGGCTTCCAGAAGATGTGATGGCCAGGCCGACTGTGACGA
  TGACAGTGATGAGGAAAACTGTGGTTGTAAAGAGAGAGATCTTTGGGAATGTCCATCCAATAAACAATGT
  TTGAAGCACACAGTGATCTGCGATGGGTTCCCAGACTGCCCTGATTACATGGACGAGAAAAACTGCTCAT
  TTTGCCAAGATGATGAGCTGGAATGTGCAAACCATGCGTGTGTGTCACGTGACCTGTGGTGTGATGGTGA
  AGCCGACTGCTCAGACAGTTCAGATGAATGGGACTGTGTGACCCTCTCTATAAATGTGAACTCCTCTTCC
  TTTCTGATGGTTCACAGAGCTGCCACAGAACACCATGTGTGTGCAGATGGCTGGCAGGAGATATTGAGTC
  AGCTGGCCTGCAAGCAGATGGGTTTAGGAGAACCATCTGTGACCAAATTGATACAGGAACAGGAGAAAGA
  GCCGCGGTGGCTGACATTACACTCCAACTGGGAGAGCCTCAATGGGACCACTTTACATGAACTTCTAGTA
  AATGGGCAGTCTTGTGAGAGCAGAAGTAAAATTTCTCTTCTGTGTACTAAACAAGACTGTGGGCGCCGCC
  CTGCTGCCCGAATGAACAAAAGGATCCTTGGAGGTCGGACGAGTCGCCCTGGAAGGTGGCCATGGCAGTG
  TTCTCTGCAGAGTGAACCCAGTGGACATATCTGTGGCTGTGTCCTCATTGCCAAGAAGTGGGTTCTGACA
  GTTGCCCACTGCTTCGAGGGGAGAGAGAATGCTGCAGTTTGGAAAGTGGTGCTTGGCATCAACAATCTAG
  ACCATCCATCAGTGTTCATGCAGACACGCTTTGTGAAGACCATCATCCTGCATCCCCGCTACAGTCGAGC
  AGTGGTGGACTATGACATCAGCATCGTTGAGCTGAGTGAAGACATCAGTGAGACTGGCTACGTCCGGCCT
  GTCTGCTTGCCCAACCCGGAGCAGTGGCTAGAGCCTGACACGTACTGCTATATCACAGGCTGGGGCCACA
  TGGGCAATAAAATGCCATTTAAGCTGCAAGAGGGAGAGGTCCGCATTATTTCTCTGGAACATTGTCAGTC
  CTACTTTGACATGAAGACCATCACCACTCGGATGATATGTGCTGGCTATGAGTCTGGCACAGTTGATTCA
  TGCATGGGTGACAGCGGTGGGCCTCTTGTTTGTGAGAAGCCTGGAGGACGGTGGACATTATTTGGATTAA
  CTTCATGGGGCTCCGTCTGCTTTTCCAAAGTCCTGGGGCCTGGCGTTTATAGTAATGTGTCATATTTCGT
  CGAATGGATTAAAAGACAGATTTACATCCAGACCTTTCTCCTAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212742 representing NM_006587
  Red=Cloning site Green=Tags(s)
  
  MKQSPALAPEERCRRAGSPKPVLRADDNNMGNGCSQKLATANLLRFLLLVLIPCICALVLLLVILLSYVG
  TLQKVYFKSNGSEPLVTDGEIQGSDVILTNTIYNQSTVVSTAHPDQHVPAWTTDASLPGDQSHRNTSACM
  NITHSQCQMLPYHATLTPLLSVVRNMEMEKFLKFFTYLHRLSCYQHIMLFGCTLAFPECIIDGDDSHGLL
  PCRSFCEAAKEGCESVLGMVNYSWPDFLRCSQFRNQTESSNVSRICFSPQQENGKQLLCGRGENFLCASG
  ICIPGKLQCNGYNDCDDWSDEAHCNCSENLFHCHTGKCLNYSLVCDGYDDCGDLSDEQNCDCNPTTEHRC
  GDGRCIAMEWVCDGDHDCVDKSDEVNCSCHSQGLVECRNGQCIPSTFQCDGDEDCKDGSDEENCSVIQTS
  CQEGDQRCLYNPCLDSCGGSSLCDPNNSLNNCSQCEPITLELCMNLPYNSTSYPNYFGHRTQKEASISWE
  SSLFPALVQTNCYKYLMFFSCTILVPKCDVNTGEHIPPCRALCEHSKERCESVLGIVGLQWPEDTDCSQF
  PEENSDNQTCLMPDEYVEECSPSHFKCRSGQCVLASRRCDGQADCDDDSDEENCGCKERDLWECPSNKQC
  LKHTVICDGFPDCPDYMDEKNCSFCQDDELECANHACVSRDLWCDGEADCSDSSDEWDCVTLSINVNSSS
  FLMVHRAATEHHVCADGWQEILSQLACKQMGLGEPSVTKLIQEQEKEPRWLTLHSNWESLNGTTLHELLV
  NGQSCESRSKISLLCTKQDCGRRPAARMNKRILGGRTSRPGRWPWQCSLQSEPSGHICGCVLIAKKWVLT
  VAHCFEGRENAAVWKVVLGINNLDHPSVFMQTRFVKTIILHPRYSRAVVDYDISIVELSEDISETGYVRP
  VCLPNPEQWLEPDTYCYITGWGHMGNKMPFKLQEGEVRIISLEHCQSYFDMKTITTRMICAGYESGTVDS
  CMGDSGGPLVCEKPGGRWTLFGLTSWGSVCFSKVLGPGVYSNVSYFVEWIKRQIYIQTFLLN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006587
• ORF Size: 3126 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006587.4
• RefSeq Size: 4942 bp
• RefSeq ORF: 3129 bp
• Locus ID: 10699
• UniProt ID: Q9Y5Q5
• Cytogenetics: 4p12
• Domains: FRI, SR, Tryp_SPc, ldl_recept_a
• Protein Families: Druggable Genome, Protease, Transmembrane
• MW: 116.3 kDa
• Gene Summary: This gene encodes a member of the type II transmembrane serine protease class of the trypsin superfamily. Members of this family are composed of multiple structurally distinct domains. The encoded protein converts pro-atrial natriuretic peptide to biologically active atrial natriuretic peptide, a cardiac hormone that regulates blood volume and pressure. This protein may also function as a pro-brain-type natriuretic peptide convertase. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]