EVX2 (NM_001080458) Human Tagged ORF Clone

CAT#: RC212735

EVX2 (Myc-DDK-tagged)-Human even-skipped homeobox 2 (EVX2)

Product Images

Circular map for RC212735

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EVX2
• Synonyms: EVX-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212735 representing NM_001080458
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAAAGAATAAGAAAAGAGATGATTCTGATGGAGAGAGGGCTGCACAGCCCTACGGCGGGCAAGA
  GATTCTCCAATTTGTCCAACTCGGCTGGCAATGCTGTGCTCGAGGCCCTGGAAAATTCGCAGCACCCGGC
  TCGCCTAAGCCCGCGCCTGCCGTCTGCCCCCCTGCACAGCGCTCTGGGAGAACTCCCCGCCAAGGGCAAA
  TTCGAAATAGACACTTTGTTCAACCTGCAGCACACGGGCAGCGAAAGCACCGTCTCCTCCGAAATCTCCT
  CCGCCGCCGAGAGCCGCAAGAAGCCGGGCCATTATTCAGAGGCGGCCGCTGAGGCCGACATGAGCAGCGA
  CGTGGAGGTGGGCTGCTCCGCGCTTCGCTCCCCCGGGGGCCTCGGCGCCGCTCAGCTTAAGGAAAACAAT
  GGCAAAGGGTACGCAGAGAGCGGCTCGGCTGCCGGCACCACGACGTCGGCGTCGGGCTCAGGCCTCGGAA
  GCCTGCATGGAGGCAGCGGAGGCAGCGGCGGGAGCGCGGCGCTGGGTGGCTCCGGCTCTGGCGCGGATCA
  AGTGCGGCGCTACCGTACGGCGTTCACCCGCGAGCAGATCGCGCGCCTGGAGAAGGAGTTCTACCGGGAG
  AACTATGTGTCGCGGCCCCGCCGGTGCGAGCTGGCCGCGGCACTCAACCTGCCCGAAACCACCATCAAGG
  TGTGGTTCCAGAACCGGCGCATGAAGGACAAGCGGCAGCGCCTGGCCATGTCCTGGCCGCACCCAGCCGA
  CCCCAGCTTCTACACCTACATGATGACGCACGCGGCCGCCACCGGAAGCCTGCCCTACCCCTTCCACTCG
  CACGTGCCGCTGCACTACTACCCGCACGTGGGCGTCACGGCGGCGGCGGCCGCGGCTGCAGCCTCAGGCG
  CGGCGGCCGCGGCTTCGTCGCCCTTCGCTACTTCCATCCGGCCACTGGACACCTTCCGCGCCCTCTCGCA
  CCCCTACTCTCGGCCGGAGCTGCTGTGTAGCTTCCGCCACCCTGGTCTCTACCAGGCTCCCGCGGCCGCC
  GCGGGGCTCAACAGCGCGGCCTCTGCCGCGGCAGCCGCGGCAGCCGCAGCGGCTGCGGCCTCCTCGGCGG
  CGGCGGCCGGCGCGCCCCCCAGCGGCGGCTCTGCACCCTGCTCGTGCCTCAGTTGCCACAGCAGTCAGTC
  GGCGGCGGCAGCCGCGGCAGCAGCTGCCGCAGCCCTGGGTTCCCGGGGTGGCGGTGGCGGCGGCGGTGGT
  GGTGGTGGCGGCGGCGGCGGGGGCGCCGGGGCCGGGGGAGGCTCGGACTTCGGCTGCAGCGCGGCGGCGC
  CGCGTTCCGAGAGCGGCTTCCTGCCCTACTCGGCCGCGGTGCTTAGTAAGACGGCCGTGAGCCCGCCGGA
  CCAGAGGGACGAGGCTCCGCTCACCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212735 representing NM_001080458
  Red=Cloning site Green=Tags(s)
  
  MMERIRKEMILMERGLHSPTAGKRFSNLSNSAGNAVLEALENSQHPARLSPRLPSAPLHSALGELPAKGK
  FEIDTLFNLQHTGSESTVSSEISSAAESRKKPGHYSEAAAEADMSSDVEVGCSALRSPGGLGAAQLKENN
  GKGYAESGSAAGTTTSASGSGLGSLHGGSGGSGGSAALGGSGSGADQVRRYRTAFTREQIARLEKEFYRE
  NYVSRPRRCELAAALNLPETTIKVWFQNRRMKDKRQRLAMSWPHPADPSFYTYMMTHAAATGSLPYPFHS
  HVPLHYYPHVGVTAAAAAAAASGAAAAASSPFATSIRPLDTFRALSHPYSRPELLCSFRHPGLYQAPAAA
  AGLNSAASAAAAAAAAAAAASSAAAAGAPPSGGSAPCSCLSCHSSQSAAAAAAAAAAALGSRGGGGGGGG
  GGGGGGGGAGAGGGSDFGCSAAAPRSESGFLPYSAAVLSKTAVSPPDQRDEAPLTR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080458
• ORF Size: 1428 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080458.1, NP_001073927.1
• RefSeq Size: 1617 bp
• RefSeq ORF: 1431 bp
• Locus ID: 344191
• UniProt ID: Q03828
• Cytogenetics: 2q31.1
• MW: 47.6 kDa
• Gene Summary: This gene is located at the 5' end of the HOXD gene cluster on chromosome 2. The encoded protein is a homeobox transcription factor that is related to the protein encoded by the Drosophila even-skipped (eve) gene, a member of the pair-rule class of segmentation genes. A 117 kb microdeletion at the 5' end of the HOXD gene cluster, which includes this gene and the HOXD9-HOXD13 genes, causes synpolydactyly, a dominantly inherited disease resulting in limb malformation. [provided by RefSeq, Sep 2009]