PIAS2 (NM_004671) Human Tagged ORF Clone

CAT#: RC212659

PIAS2 (Myc-DDK-tagged)-Human protein inhibitor of activated STAT, 2 (PIAS2), transcript variant beta

Product Images

Circular map for RC212659

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PIAS2 (Cat# RC212659, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIAS2 (Cat# TA808334)(1:500). Positive lysates LY401487 (100ug) and LC401487 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY401487) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212659 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PIAS2
• Synonyms: ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212659 representing NM_004671
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATTTCGAAGAGTTGAGGAATATGGTTTCTAGTTTTAGGGTTTCTGAACTACAAGTATTACTAG
  GCTTTGCTGGACGGAATAAAAGTGGACGCAAGCATGACCTCCTGATGAGGGCGCTGCATTTATTGAAGAG
  CGGCTGCAGCCCTGCGGTTCAGATTAAAATCCGAGAATTGTATAGACGCCGATATCCACGAACTCTTGAA
  GGACTTTCTGATTTATCCACAATCAAATCATCGGTTTTCAGTTTGGATGGTGGCTCATCACCTGTAGAAC
  CTGACTTGGCCGTGGCTGGAATCCACTCGTTGCCTTCCACTTCAGTTACACCTCACTCACCATCCTCTCC
  TGTTGGTTCTGTGCTGCTTCAAGATACTAAGCCCACATTTGAGATGCAGCAGCCATCTCCCCCAATTCCT
  CCTGTCCATCCTGATGTGCAGTTAAAAAATCTGCCCTTTTATGATGTCCTTGATGTTCTCATCAAGCCCA
  CGAGTTTAGTTCAAAGCAGTATTCAGCGATTTCAAGAGAAGTTTTTTATTTTTGCTTTGACACCTCAACA
  AGTTAGAGAGATATGCATATCCAGGGATTTTTTGCCAGGTGGTAGGAGAGATTATACAGTCCAAGTTCAG
  TTGAGACTTTGCCTGGCAGAGACAAGTTGCCCTCAAGAAGATAACTATCCAAATAGTCTATGTATAAAAG
  TAAATGGGAAGCTATTTCCTTTGCCTGGCTATGCACCACCGCCTAAAAATGGGATTGAACAGAAGCGCCC
  TGGACGCCCCTTGAATATTACATCTTTAGTTAGGTTATCTTCAGCTGTGCCAAACCAAATTTCCATTTCT
  TGGGCATCAGAAATTGGGAAGAATTACTCTATGTCTGTATATCTTGTACGGCAGCTTACATCAGCCATGT
  TATTACAGAGATTAAAAATGAAAGGTATTAGAAACCCTGATCATTCCAGAGCACTAATTAAAGAAAAACT
  TACTGCAGATCCTGATAGTGAAATTGCTACAACTAGCCTTCGGGTATCCTTGATGTGCCCTTTAGGAAAA
  ATGAGGCTGACAATCCCATGCCGTGCAGTGACTTGTACACATCTGCAGTGTTTTGATGCTGCCCTCTATC
  TACAAATGAATGAGAAAAAGCCCACCTGGATTTGTCCTGTGTGTGACAAAAAAGCTGCCTATGAAAGTCT
  AATATTAGATGGGCTTTTTATGGAAATTCTCAATGACTGTTCTGATGTAGATGAGATCAAATTCCAAGAA
  GATGGTTCTTGGTGTCCAATGAGACCGAAGAAAGAAGCTATGAAAGTATCCAGCCAACCGTGTACAAAAA
  TAGAAAGTTCAAGCGTCCTCAGTAAGCCTTGTTCAGTGACTGTAGCCAGTGAGGCAAGCAAGAAGAAAGT
  AGATGTTATTGATCTTACAATAGAAAGCTCTTCTGACGAAGAGGAAGACCCTCCTGCCAAAAGGAAATGC
  ATCTTTATGTCAGAAACACAAAGCAGCCCAACCAAAGGGGTTCTCATGTATCAGCCATCTTCTGTAAGGG
  TGCCCAGTGTGACTTCGGTTGATCCTGCTGCTATTCCGCCTTCATTAACAGACTACTCAGTACCATTCCA
  CCATACGCCAATATCAAGCATGTCATCAGATTTGCCAGGTTTGGATTTTCTTTCCCTTATTCCAGTTGAT
  CCCCAGTACTGTCCTCCTATGTTTTTGGATAGTCTCACCTCACCCTTAACAGCAAGCAGTACGTCTGTCA
  CCACCACCAGCTCCCATGAAAGCAGTACTCATGTTAGTTCATCCAGCAGCAGGAGTGAGACAGGGGTCAT
  AACCAGCAGTGGAAGTAACATTCCTGACATCATCTCATTGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212659 representing NM_004671
  Red=Cloning site Green=Tags(s)
  
  MADFEELRNMVSSFRVSELQVLLGFAGRNKSGRKHDLLMRALHLLKSGCSPAVQIKIRELYRRRYPRTLE
  GLSDLSTIKSSVFSLDGGSSPVEPDLAVAGIHSLPSTSVTPHSPSSPVGSVLLQDTKPTFEMQQPSPPIP
  PVHPDVQLKNLPFYDVLDVLIKPTSLVQSSIQRFQEKFFIFALTPQQVREICISRDFLPGGRRDYTVQVQ
  LRLCLAETSCPQEDNYPNSLCIKVNGKLFPLPGYAPPPKNGIEQKRPGRPLNITSLVRLSSAVPNQISIS
  WASEIGKNYSMSVYLVRQLTSAMLLQRLKMKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLMCPLGK
  MRLTIPCRAVTCTHLQCFDAALYLQMNEKKPTWICPVCDKKAAYESLILDGLFMEILNDCSDVDEIKFQE
  DGSWCPMRPKKEAMKVSSQPCTKIESSSVLSKPCSVTVASEASKKKVDVIDLTIESSSDEEEDPPAKRKC
  IFMSETQSSPTKGVLMYQPSSVRVPSVTSVDPAAIPPSLTDYSVPFHHTPISSMSSDLPGLDFLSLIPVD
  PQYCPPMFLDSLTSPLTASSTSVTTTSSHESSTHVSSSSSRSETGVITSSGSNIPDIISLD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004671
• ORF Size: 1863 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004671.5
• RefSeq Size: 2360 bp
• RefSeq ORF: 1866 bp
• Locus ID: 9063
• UniProt ID: O75928
• Cytogenetics: 18q21.1
• Domains: SAP, zf-MIZ
• Protein Families: Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors
• Protein Pathways: Jak-STAT signaling pathway, Pathways in cancer, Small cell lung cancer, Ubiquitin mediated proteolysis
• MW: 68.1 kDa
• Gene Summary: This gene encodes a member of the protein inhibitor of activated STAT family, which function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Isoforms of the encoded protein enhance the sumoylation of specific target proteins including the p53 tumor suppressor protein, c-Jun, and the androgen receptor. A pseudogene of this gene is located on the short arm of chromosome 4. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. [provided by RefSeq, Aug 2017]