p16 ARC (ARPC5) (NM_005717) Human Tagged ORF Clone
ARPC5 (Myc-DDK-tagged)-Human actin related protein 2/3 complex, subunit 5, 16kDa (ARPC5)
|Product Name||p16 ARC (ARPC5) (NM_005717) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||ARC16; dJ127C7.3; p16-Arc|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC212631 representing NM_005717
Red=Cloning site Blue=ORF Green=Tags(s)
>RC212631 representing NM_005717
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||453 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_005717.1, NM_005717.2, NM_005717.3, NP_005708.1|
|RefSeq Size||2000 bp|
|RefSeq ORF||456 bp|
|Protein Pathways||Fc gamma R-mediated phagocytosis, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton|
|Gene Summary||This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p16 subunit, has yet to be determined. Alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]|
|The use of this cDNA Clones has been cited in the following citations:|
Interaction between microRNAs and actin-associated protein Arpc5 regulates translational suppression during male germ cell differentiation
,Yao-Fu Chang, Jennifer S. Lee-Chang, J. Saadi Imam, Kalyan Chakravarthy Buddavarapu, Sarah S. Subaran, Amiya P. Sinha-Hikim, Myriam Gorospe, and Manjeet K. Rao,
PNAS, Mar 2012; 10.1073/pnas.1117837109.
|cDNA Clone Resources|
|SC112682||ARPC5 (untagged)-Human actin related protein 2/3 complex, subunit 5, 16kDa (ARPC5)||
|RG212631||ARPC5 (GFP-tagged) - Human actin related protein 2/3 complex, subunit 5, 16kDa (ARPC5)||
|RC212631L3||Lenti ORF clone of Human actin related protein 2/3 complex, subunit 5, 16kDa (ARPC5), Myc-DDK-tagged||
|RC212631L4||Lenti ORF clone of Human actin related protein 2/3 complex, subunit 5, 16kDa (ARPC5), mGFP tagged||