SGCE (NM_001099400) Human Tagged ORF Clone

CAT#: RC212553

SGCE (Myc-DDK-tagged)-Human sarcoglycan, epsilon (SGCE), transcript variant 3

Product Images

Circular map for RC212553

• 

  Western blot validation of overexpression lysate (Cat# LY420437) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212553 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SGCE
• Synonyms: DYT11; epsilon-SG; ESG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212553 representing NM_001099400
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAATTGCCCCGGTGGTGGGAGCTGGGAGACCCCTGTGCTTGGACGGGACAGGGTCGGGGGACACGCA
  GGATGAGCCCCGCGACCACTGGCACATTCTTGCTGACAGTGTACAGTATTTTCTCCAAGGTACACTCCGA
  TCGGAGTGTATACCCATCAGCAGGTGTCCTCTTTGTTCATGTTTTGGAAAGAGAATATTTTAAGGGGGAA
  TTTCCACCTTACCCAAAACCTGGCGAGATTAGTAATGATCCCATAACATTTAATACAAATTTAATGGGTT
  ACCCAGACCGACCTGGATGGCTTCGATATATCCAAAGGACACCATATAGTGATGGAGTCCTATATGGGTC
  CCCAACAGCTGAAAATGTGGGGAAGCCAACAATCATTGAGATAACTGCCTACAACAGGCGCACCTTTGAG
  ACTGCAAGGCATAATTTGATAATTAATATAATGTCTGCAGAAGACTTCCCGTTGCCATATCAAGCAGAAT
  TCTTCATTAAGAATATGAATGTAGAAGAAATGTTGGCCAGTGAGGTTCTTGGAGACTTTCTTGGCGCAGT
  GAAAAATGTGTGGCAGCCAGAGCGCCTGAACGCCATAAACATCACATCGGCCCTAGACAGGGGTGGCAGG
  GTGCCACTTCCCATTAATGACCTGAAGGAGGGCGTTTATGTCATGGTTGGTGCAGATGTCCCGTTTTCTT
  CTTGTTTACGAGAAGTTGAAAATCCACAGAATCAATTGAGATGTAGTCAAGAAATGGAGCCTGTAATAAC
  ATGTGATAAAAAATTTCGTACTCAATTTTACATTGACTGGTGCAAAATTTCATTGGTTGATAAAACAAAG
  CAAGTGTCCACCTATCAGGAAGTGATTCGTGGAGAGGGGATTTTACCTGATGGTGGAGAATACAAACCCC
  CTTCTGATTCTTTGAAAAGCAGAGACTATTACACGGATTTCCTAATTACACTGGCTGTGCCCTCGGCAGT
  GGCACTGGTCCTTTTTCTAATACTTGCTTATATCATGTGCTGCCGACGGGAAGGCGTCATCCAACTGGTC
  CATCACAGTGCTATTCAGAAATCTACCAAGGAGCTTCGAGACATGTCCAAGAATAGAGAGATAGCATGGC
  CCCTGTCAACGCTTCCTGTGTTCCACCCTGTGACTGGGGAAATCATACATCCTTTACACACAGACAACTA
  TGATAGCACAAACATGCCATTGATGCAAACGCAGCAGAACTTGCCACATCAGACTCAGATTCCCCAACAG
  CAGACTACAGGAGATTTTCGTTTGACAACTTTTCAAAGATTTGAGGTAAATGGTATCCCTGAAGAAAGAA
  AACTGACTGAAGCAATGAATTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212553 representing NM_001099400
  Red=Cloning site Green=Tags(s)
  
  MQLPRWWELGDPCAWTGQGRGTRRMSPATTGTFLLTVYSIFSKVHSDRSVYPSAGVLFVHVLEREYFKGE
  FPPYPKPGEISNDPITFNTNLMGYPDRPGWLRYIQRTPYSDGVLYGSPTAENVGKPTIIEITAYNRRTFE
  TARHNLIINIMSAEDFPLPYQAEFFIKNMNVEEMLASEVLGDFLGAVKNVWQPERLNAINITSALDRGGR
  VPLPINDLKEGVYVMVGADVPFSSCLREVENPQNQLRCSQEMEPVITCDKKFRTQFYIDWCKISLVDKTK
  QVSTYQEVIRGEGILPDGGEYKPPSDSLKSRDYYTDFLITLAVPSAVALVLFLILAYIMCCRREGVIQLV
  HHSAIQKSTKELRDMSKNREIAWPLSTLPVFHPVTGEIIHPLHTDNYDSTNMPLMQTQQNLPHQTQIPQQ
  QTTGDFRLTTFQRFEVNGIPEERKLTEAMNL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001099400
• ORF Size: 1353 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001099400.1, NP_001092870.1
• RefSeq Size: 1717 bp
• RefSeq ORF: 1356 bp
• Locus ID: 8910
• UniProt ID: O43556
• Cytogenetics: 7q21.3
• Protein Families: Transmembrane
• MW: 51.2 kDa
• Gene Summary: This gene encodes the epsilon member of the sarcoglycan family. Sarcoglycans are transmembrane proteins that are components of the dystrophin-glycoprotein complex, which link the actin cytoskeleton to the extracellular matrix. Unlike other family members which are predominantly expressed in striated muscle, the epsilon sarcoglycan is more broadly expressed. Mutations in this gene are associated with myoclonus-dystonia syndrome. This gene is imprinted, with preferential expression from the paternal allele. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A pseudogene associated with this gene is located on chromosome 2. [provided by RefSeq, Oct 2016]