Zfp219 (ZNF219) (NM_001102454) Human Tagged ORF Clone

CAT#: RC212535

ZNF219 (Myc-DDK-tagged)-Human zinc finger protein 219 (ZNF219), transcript variant 3

Product Images

Circular map for RC212535

• 

  Western blot validation of overexpression lysate (Cat# LY420325) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223274 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zfp219
• Synonyms: ZFP219
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212535 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGCTCACGTCCCCGCGCCCCGAGCGGCCACTTAGCGCCGTCGCCGCCGGCTTTCGACGGCGAGC
  TGGATCTGCAGCGATACTCCAACGGGCCAGCCGTGAGCGCAGGGTCGCTCGGGATGGGAGCGGTGAGCTG
  GTCTGAGAGTCGTGCAGGCGAACGGCGCTTCCCCTGCCCTGTATGCGGGAAGCGCTTCCGCTTCAACTCT
  ATCCTTGCTTTGCACCTGCGGGCGCACCCAGGAGCCCAGGCCTTCCAGTGCCCTCACTGCGGCCACCGCG
  CGGCGCAGCGGGCTCTGCTGCGCTCGCACCTGCGCACACACCAGCCCGAGCGCCCACGTAGTCCTGCTGC
  ACGCCTGTTGCTGGAGTTGGAAGAGCGCGCGCTACTACGCGAGGCCCGACTGGGGAGAGCCCGAAGCTCA
  GGGGGCATGCAGGCCACCCCTGCCACTGAGGGTCTGGCGCGGCCCCAGGCTCCTTCATCGTCCGCCTTCC
  GTTGCCCCTACTGCAAAGGCAAGTTTCGCACCTCGGCGGAGCGCGAACGCCACCTGCACATCCTGCATAG
  GCCCTGGAAGTGCGGCCTGTGCAGTTTCGGCTCCAGCCAGGAGGAGGAGCTGCTGCACCACAGCCTGACG
  GCCCACGGGGCTCCCGAGCGTCCCCTGGCGGCCACCTCCGCTGCGCCTCCGCCTCAGCCTCAGCCTCCAC
  CCCAGCCCGAACCCAGATCAGTTCCCCAGCCGGAGCCGGAGCCGGAGCCCGAACGTGAGGCAACCCCGAC
  CCCAGCTCCTGCCGCTCCCGAGGAGCCCCCAGCGCCTCCGGAGTTCCGCTGCCAAGTGTGCGGCCAGAGC
  TTTACACAGTCTTGGTTTCTCAAGGGCCACATGCGTAAGCACAAGGCCTCCTTCGATCATGCGTGTCCGG
  TGTGCGGCCGCTGCTTCAAGGAGCCCTGGTTCCTTAAGAACCACATGAAGGTGCACGCCAGCAAGCTGGG
  CCCACTGCGTGCCCCGGGGCCTGCCTCCGGGCCTGCCCGCGCCCCCCAGCCTCCTGACCTCGGCCTGCTG
  GCCTATGAGCCGTTGGGCCCAGCGCTCCTCTTGGCCCCGGCTCCCACCCCGGCCGAGCGCCGTGAGCCCC
  CGAGCCTCTTGGGCTACCTGAGCCTGCGAGCTGGCGAGGGCCGGCCCAACGGCGAGGGTGCTGAGCCCGG
  TCCCGGCCGCAGCTTCGGAGGCTTCCGCCCGCTGTCCTCTGCTCTCCCGGCCCGGGCTCGCCGGCACCGT
  GCGGAGGAGCCTGAGGAAGAAGAGGAGGTGGTGGAGGCCGAGGAGGAAACCTGGGCCCGGGGCAGGTCGC
  TGGGCTCTCTGGCTTCCCTGCACCCGCGCCCGGGTGAGGGACCGGGGCACTCTGCCTCTGCTGCTGGGGC
  CCAGGCAAGATCGACCGCCACGCAGGAAGAGAATGGGCTGTTGGTTGGAGGGACCCGGCCTGAAGGGGGC
  CGGGGCGCCACCGGCAAGGATTGTCCTTTCTGCGGAAAATCTTTCCGCTCAGCACATCACCTCAAAGTGC
  ATCTGCGAGTGCACACAGGCGAGCGGCCCTACAAGTGTCCGCACTGCGACTACGCGGGCACCCAGTCCGG
  CTCGCTCAAGTATCACCTACAGCGCCACCACCGGGAGCAGAGGAGCGGGGCCGGCCCCGGGCCACCCCCG
  GAGCCACCGCCTCCTTCCCAGCGGGGTTCGGCCCCGCAATCTGGAGCCAAGCCGTCTCCGCAGCCTGCGA
  CCTGGGTGGAGGGCGCCTCAAGTCCCCGGCCTCCTTCTAGCGGTGCTGGGCCGGGGTCCCGTCGGAAGCC
  CGCCAGCCCTGGGAGGACCCTGCGCAACGGGCGAGGCGGTGAGGCCGAACCCCTGGACCTGTCCTTGCGG
  GCAGGGCCGGGAGGCGAGGCCGGGCCTGGGGGTGCCCTCCACCGCTGCCTCTTCTGCCCGTTCGCCACTG
  GAGCCCCAGAGCTCATGGCCTTGCACCTTCAAGTGCACCACAGCCGCCGGGCTAGGGGCCGCCGGCCACC
  CCAGGCTGACGCGTCCCCGCCCTATGCCCGAGTACCATCAGGAGAGACCCCTCCCAGTCCTTCGCAGGAA
  GGGGAGGAGGGCTCCGGGCTGTCCAGACCCGGAGAGGCAGGGCTGGGGGGGCAAGAACGG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212535 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEGSRPRAPSGHLAPSPPAFDGELDLQRYSNGPAVSAGSLGMGAVSWSESRAGERRFPCPVCGKRFRFNS
  ILALHLRAHPGAQAFQCPHCGHRAAQRALLRSHLRTHQPERPRSPAARLLLELEERALLREARLGRARSS
  GGMQATPATEGLARPQAPSSSAFRCPYCKGKFRTSAERERHLHILHRPWKCGLCSFGSSQEEELLHHSLT
  AHGAPERPLAATSAAPPPQPQPPPQPEPRSVPQPEPEPEPEREATPTPAPAAPEEPPAPPEFRCQVCGQS
  FTQSWFLKGHMRKHKASFDHACPVCGRCFKEPWFLKNHMKVHASKLGPLRAPGPASGPARAPQPPDLGLL
  AYEPLGPALLLAPAPTPAERREPPSLLGYLSLRAGEGRPNGEGAEPGPGRSFGGFRPLSSALPARARRHR
  AEEPEEEEEVVEAEEETWARGRSLGSLASLHPRPGEGPGHSASAAGAQARSTATQEENGLLVGGTRPEGG
  RGATGKDCPFCGKSFRSAHHLKVHLRVHTGERPYKCPHCDYAGTQSGSLKYHLQRHHREQRSGAGPGPPP
  EPPPPSQRGSAPQSGAKPSPQPATWVEGASSPRPPSSGAGPGSRRKPASPGRTLRNGRGGEAEPLDLSLR
  AGPGGEAGPGGALHRCLFCPFATGAPELMALHLQVHHSRRARGRRPPQADASPPYARVPSGETPPSPSQE
  GEEGSGLSRPGEAGLGGQER
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001102454
• ORF Size: 1389 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001102454.1, NP_001095924.1
• RefSeq Size: 2954 bp
• RefSeq ORF: 2169 bp
• Locus ID: 51222
• UniProt ID: Q9P2Y4
• Cytogenetics: 14q11.2
• Protein Families: Transcription Factors
• MW: 76.7 kDa
• Gene Summary: This gene is a member of the Kruppel-like zinc finger gene family. The encoded protein functions as a transcriptional repressor of the high mobility group nucleosome binding domain 1 protein, which is associated with transcriptionally active chromatin. [provided by RefSeq, Apr 2017]