CRYGN (NM_144727) Human Tagged ORF Clone

CAT#: RC212423

CRYGN (Myc-DDK-tagged)-Human crystallin, gamma N (CRYGN)

Product Images

Circular map for RC212423

• 

  Western blot validation of overexpression lysate (Cat# LY408155) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212423 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CRYGN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212423 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCAGCGCTCGGGGAAGATCACTCTCTATGAAGGCAAGCACTTCACAGGGCAGAAGCTGGAGGTCT
  TCGGGGACTGTGACAACTTCCAGGACCGGGGCTTTATGAACCGAGTGAACTCCATCCACGTGGAGAGCGG
  AGCCTGGGTCTGCTTCAATCACCCCGACTTCCGGGGCCAGCAGTTCATCTTGGAGCACGGCGACTACCCC
  GACTTCTTCCGCTGGAACAGCCACAGTGACCACATGGGCTCCTGTCGGCCTGTAGGAATGCACGGAGAAC
  ATTTCCGCCTAGAAATCTTCGAGGGTTGCAACTTCACGGGCCAGTGCCTGGAGTTCCTGGAGGACAGCCC
  CTTCCTCCAGAGCAGGGGCTGGGTCAAGAACTGTGTGAACACCATCAAGGTGTACGGGGACGGAGCAGCA
  TGGAGCCCTAGAAGCTTCGGAGCTGAGGACTTCCAGCTGAGCAGCTCTCTTCAATCAGATCAAGGACCGG
  AAGAGGCCACCACAAAACCAGCAACAACCCAGCCACCTTTCCTGACTGCAAACCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212423 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAQRSGKITLYEGKHFTGQKLEVFGDCDNFQDRGFMNRVNSIHVESGAWVCFNHPDFRGQQFILEHGDYP
  DFFRWNSHSDHMGSCRPVGMHGEHFRLEIFEGCNFTGQCLEFLEDSPFLQSRGWVKNCVNTIKVYGDGAA
  WSPRSFGAEDFQLSSSLQSDQGPEEATTKPATTQPPFLTANL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144727
• ORF Size: 546 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144727.3
• RefSeq Size: 746 bp
• RefSeq ORF: 549 bp
• Locus ID: 155051
• UniProt ID: Q8WXF5
• Cytogenetics: 7q36.1
• MW: 20.6 kDa
• Gene Summary: This gene encodes a member of the crystallin family of proteins that are localized to the refractive structure of vertebrate eye lenses. The protein encoded by this gene is unique in that it has both beta and gamma crystallin protein motifs. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2015]