IRX5 (NM_005853) Human Tagged ORF Clone

CAT#: RC212385

IRX5 (Myc-DDK-tagged)-Human iroquois homeobox 5 (IRX5)

Product Images

Circular map for RC212385

• 

  Western blot validation of overexpression lysate (Cat# LY417041) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212385 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IRX5
• Synonyms: HMMS; IRX-2a; IRXB2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212385 representing NM_005853
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTATCCGCAGGGCTACTTGTACCAGCCGTCCGCCTCGCTGGCGCTCTACTCGTGCCCGGCGTACA
  GCACCAGCGTCATTTCGGGGCCCCGCACGGATGAGCTCGGCCGCTCTTCTTCGGGCTCCGCGTTCTCGCC
  CTACGCTGGCTCGACTGCCTTCACGGCGCCCTCGCCGGGCTACAACTCGCACCTCCAGTACGGCGCCGAC
  CCCGCGGCCGCCGCCGCCGCCGCCTTCTCCTCGTACGTGGGCTCTCCCTACGACCACACACCCGGCATGG
  CGGGCTCCTTGGGGTACCATCCTTACGCGGCGCCCCTGGGATCGTACCCTTACGGGGACCCAGCGTACCG
  GAAGAACGCCACAAGGGACGCCACGGCTACCCTCAAGGCCTGGCTCAACGAGCACCGCAAGAACCCCTAC
  CCCACCAAGGGCGAGAAGATCATGCTGGCCATCATCACCAAGATGACCCTCACCCAGGTGTCCACCTGGT
  TCGCCAACGCGCGCCGGCGCCTCAAGAAAGAGAATAAAATGACGTGGACGCCGCGGAACCGCAGCGAGGA
  CGAGGAAGAGGAGGAGAACATTGACCTGGAGAAGAACGACGAGGACGAGCCCCAGAAGCCCGAGGACAAG
  GGCGACCCCGAGGGCCCCGAAGCAGGAGGAGCTGAGCAGAAGGCGGCTTCGGGCTGCGAACGGCTTCAGG
  GACCACCCACCCCTGCAGGCAAGGAGACGGAGGGCAGCCTCAGCGACTCGGATTTTAAGGAGCCGCCCTC
  GGAGGGCCGCCTCGACGCGCTGCAGGGCCCCCCCCGCACCGGCGGGCCCTCCCCGGCTGGGCCAGCGGCG
  GCGCGGCTGGCGGAGGACCCGGCCCCTCACTACCCCGCCGGAGCGCCGGCGCCCGGCCCGCATCCAGCCG
  CGGGCGAGGTGCCTCCGGGTCCCGGCGGGCCCTCGGTTATCCATTCGCCGCCTCCGCCGCCGCCTCCTGC
  GGTGCTCGCCAAGCCCAAACTGTGGTCTTTGGCAGAGATCGCCACATCGTCGGACAAGGTCAAGGACGGG
  GGCGGCGGGAACGAGGGCTCTCCATGCCCACCGTGTCCCGGGCCCATAGCCGGGCAAGCCCTAGGAGGCA
  GCCGGGCGTCGCCGGCCCCGGCGCCGTCACGCTCGCCCTCGGCGCAGTGTCCTTTTCCAGGCGGGACGGT
  GCTGTCCCGGCCTCTCTACTACACCGCGCCCTTCTATCCCGGCTACACGAACTATGGCTCCTTCGGACAC
  CTTCATGGCCACCCGGGGCCCGGGCCAGGCCCCACAACCGGTCCGGGGTCTCATTTCAATGGATTAAACC
  AGACCGTGTTGAACCGAGCGGACGCTTTGGCTAAAGACCCGAAAATGTTGCGGAGCCAGTCTCAGCTAGA
  CCTGTGCAAAGACTCTCCCTATGAATTGAAGAAAGGTATGTCCGACATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212385 representing NM_005853
  Red=Cloning site Green=Tags(s)
  
  MSYPQGYLYQPSASLALYSCPAYSTSVISGPRTDELGRSSSGSAFSPYAGSTAFTAPSPGYNSHLQYGAD
  PAAAAAAAFSSYVGSPYDHTPGMAGSLGYHPYAAPLGSYPYGDPAYRKNATRDATATLKAWLNEHRKNPY
  PTKGEKIMLAIITKMTLTQVSTWFANARRRLKKENKMTWTPRNRSEDEEEEENIDLEKNDEDEPQKPEDK
  GDPEGPEAGGAEQKAASGCERLQGPPTPAGKETEGSLSDSDFKEPPSEGRLDALQGPPRTGGPSPAGPAA
  ARLAEDPAPHYPAGAPAPGPHPAAGEVPPGPGGPSVIHSPPPPPPPAVLAKPKLWSLAEIATSSDKVKDG
  GGGNEGSPCPPCPGPIAGQALGGSRASPAPAPSRSPSAQCPFPGGTVLSRPLYYTAPFYPGYTNYGSFGH
  LHGHPGPGPGPTTGPGSHFNGLNQTVLNRADALAKDPKMLRSQSQLDLCKDSPYELKKGMSDI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005853
• ORF Size: 1449 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005853.6
• RefSeq Size: 2078 bp
• RefSeq ORF: 1452 bp
• Locus ID: 10265
• UniProt ID: P78411
• Cytogenetics: 16q12.2
• Protein Families: Transcription Factors
• MW: 50.8 kDa
• Gene Summary: This gene encodes a member of the iroquois homeobox gene family, which are involved in several embryonic developmental processes. Knockout mice lacking this gene show that it is required for retinal cone bipolar cell differentiation, and that it negatively regulates potassium channel gene expression in the heart to ensure coordinated cardiac repolarization. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]