CCT6A (NM_001009186) Human Tagged ORF Clone

CAT#: RC212330

CCT6A (Myc-DDK-tagged)-Human chaperonin containing TCP1, subunit 6A (zeta 1) (CCT6A), transcript variant 2

Product Images

Circular map for RC212330

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  Western blot validation of overexpression lysate (Cat# LY422908) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212330 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CCT6A protein (Cat# TP312330). The protein was produced from HEK293T cells transfected with CCT6A cDNA clone (Cat# RC212330) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCT6A
• Synonyms: CCT-zeta; CCT-zeta-1; CCT6; Cctz; HTR3; MoDP-2; TCP-1-zeta; TCP20; TCPZ; TTCP20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212330 representing NM_001009186
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGTGAAGACCCTGAACCCCAAGGCCGAGGTGGCCCGAGCGCAGGCGGCGCTGGCGGTCAACA
  TCAGCGCAGCGCGGGGTCTGCAGGACGTGCTAAGGACCAACCTGGGGCCCAAGGGCACCATGAAGATGCT
  CGTTTCTGGCGCTGGAGACATCAAACTTACTAAAGACGGCAATGTGCTGCTTCACGAAATGGGCCTTCAT
  CCTAGAATAATCACTGAAGGATTTGAAGCTGCAAAGGAAAAGGCCCTTCAGTTTTTGGAAGAAGTCAAAG
  TAAGCAGAGAGATGGACAGGGAAACACTTATAGATGTGGCCAGAACATCTCTTCGTACTAAAGTTCATGC
  TGAACTTGCAGATGTCTTAACAGAGGCTGTAGTGGACTCCATTTTGGCCATTAAAAAGCAAGATGAACCT
  ATTGATCTCTTCATGATTGAGATCATGGAGATGAAACATAAATCTGAAACTGATACAAGCTTAATCAGAG
  GGCTTGTTTTGGACCACGGAGCACGGCATCCTGATATGAAGAAAAGGGTGGAGGATGCATACATCCTCAC
  TTGTAACGTGTCATTAGAGTATGAGAAAACAGAAGTGAATTCTGGCTTTTTTTACAAGAGTGCAGAAGAG
  AGAGAAAAACTCGTGAAAGCTGAAAGAAAATTCATTGAAGATAGGGTTAAAAAAATAATAGAACTGAAAA
  GGAAAGTCTGTGGCGATTCAGATAAAGGATTTGTTGTTATTAATCAAAAGGGAATTGACCCCTTTTCCTT
  AGATGCTCTTTCAAAAGAAGGCATAGTTGCTCTGCGCAGAGCTAAAAGGAGAAATATGGAGAGGCTGACT
  CTTGCTTGTGGTGGGGTAGCCCTGAATTCTTTTGACGACCTAAGTCCTGACTGCTTGGGACATGCAGGAC
  TTGTATATGAGTATACATTGGGAGAAGAGAAGTTTACCTTTATTGAGAAATGTAACAACCCTCGTTCTGT
  CACATTATTGATCAAAGGACCAAATAAGCACACACTCACTCAGATCAAAGATGCAGTGAGGGACGGCTTG
  AGGGCTGTCAAAAATGCTATTGATGATGGCTGTGTGGTTCCAGGTGCTGGTGCCGTGGAAGTGGCAATGG
  CAGAAGCCCTGATTAAACATAAGCCCAGTGTAAAGGGCAGGGCACAGCTTGGAGTCCAAGCATTTGCTGA
  TGCATTGCTCATTATTCCCAAGGTTCTTGCTCAGAACTCTGGTTTTGACCTTCAGGAAACATTAGTTAAA
  ATTCAAGCAGAACATTCAGAATCAGGTCAGCTTGTGGGTGTGGACCTGAACACAGGTGAGCCAATGGTGG
  CAGCAGAAGTAGGCGTATGGGATAACTATTGTGTAAAGAAACAGCTTCTTCACTCCTGCACTGTGATTGC
  CACCAACATTCTCTTGGTTGATGAGATCATGCGAGCTGGAATGTCTTCTCTGAAAGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212330 representing NM_001009186
  Red=Cloning site Green=Tags(s)
  
  MAAVKTLNPKAEVARAQAALAVNISAARGLQDVLRTNLGPKGTMKMLVSGAGDIKLTKDGNVLLHEMGLH
  PRIITEGFEAAKEKALQFLEEVKVSREMDRETLIDVARTSLRTKVHAELADVLTEAVVDSILAIKKQDEP
  IDLFMIEIMEMKHKSETDTSLIRGLVLDHGARHPDMKKRVEDAYILTCNVSLEYEKTEVNSGFFYKSAEE
  REKLVKAERKFIEDRVKKIIELKRKVCGDSDKGFVVINQKGIDPFSLDALSKEGIVALRRAKRRNMERLT
  LACGGVALNSFDDLSPDCLGHAGLVYEYTLGEEKFTFIEKCNNPRSVTLLIKGPNKHTLTQIKDAVRDGL
  RAVKNAIDDGCVVPGAGAVEVAMAEALIKHKPSVKGRAQLGVQAFADALLIIPKVLAQNSGFDLQETLVK
  IQAEHSESGQLVGVDLNTGEPMVAAEVGVWDNYCVKKQLLHSCTVIATNILLVDEIMRAGMSSLKG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009186
• ORF Size: 1458 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009186.2
• RefSeq Size: 2547 bp
• RefSeq ORF: 1461 bp
• Locus ID: 908
• UniProt ID: P40227
• Cytogenetics: 7p11.2
• MW: 53.1 kDa
• Gene Summary: The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, several pseudogenes of this gene have been located. [provided by RefSeq, Jun 2010]