ZNF473 (NM_015428) Human Tagged ORF Clone

CAT#: RC212318

ZNF473 (Myc-DDK-tagged)-Human zinc finger protein 473 (ZNF473), transcript variant 1

Product Images

Circular map for RC212318

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF473
• Synonyms: ZFP100; ZN473
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212318 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGGAATTTGTGACCCTCAAGGATGTCGGCATGGACTTCACCTTGGGAGACTGGGAGCAGCTCG
  GGCTGGAACAGGGGGACACGTTCTGGGACACAGCGTTGGACAATTGCCAGGACCTCTTCCTGCTGGACCC
  CCCAAGACCCAACCTGACCTCCCACCCAGATGGCAGTGAAGATCTGGAGCCTCTGGCAGGAGGAAGCCCA
  GAAGCAACAAGCCCTGATGTGACTGAGACCAAGAACTCTCCTCTGATGGAGGATTTCTTCGAAGAAGGAT
  TCTCCCAGGAGATTATAGAGATGTTATCCAAGGATGGCTTCTGGAACTCCAATTTCGGAGAAGCCTGTAT
  AGAGGACACCTGGTTAGATAGTTTGCTAGGCGATCCAGAAAGTCTTCTGAGGTCTGATATTGCCACCAAC
  GGGGAAAGTCCCACGGAATGCAAGAGTCATGAATTAAAGAGAGGACTCAGTCCTGTGTCCACCGTTTCCA
  CGGGAGAAGATTCCATGGTGCATAATGTTTCTGAAAAGACCCTCACACCAGCTAAGTCTAAGGAATATAG
  GGGTGAGTTTTTCTCCTACTCCGACCACAGCCAGCAGGATTCTGTTCAGGAAGGGGAGAAACCATATCAA
  TGTAGTGAATGTGGGAAAAGCTTCAGTGGGAGTTACCGTCTTACCCAGCACTGGATCACTCATACTAGGG
  AGAAACCCACTGTCCATCAAGAGTGTGAGCAAGGTTTTGACCGGAATGCTTCCCTTTCTGTGTATCCGAA
  AACTCACACGGGCTACAAATTCTATGTGTGTAATGAATATGGGACAACTTTTAGTCAGAGTACATACCTG
  TGGCATCAGAAAACTCACACTGGAGAAAAACCATGTAAGAGTCAAGATAGTGACCACCCACCCAGTCATG
  ACACACAGCCTGGTGAGCATCAGAAAACTCACACAGATAGTAAGTCCTACAACTGTAACGAATGCGGCAA
  GGCTTTTACCCGGATCTTCCACCTTACTCGGCACCAGAAGATCCACACTCGGAAACGCTATGAGTGTTCC
  AAGTGCCAGGCGACCTTCAACTTGAGAAAACACCTCATCCAACATCAGAAAACTCACGCTGCAAAAACTA
  CCTCTGAGTGTCAGGAGTGTGGGAAGATTTTTAGGCACAGTTCGCTGCTCATTGAACACCAGGCTCTTCA
  TGCTGGAGAGGAGCCTTATAAGTGTAACGAACGTGGGAAATCCTTCAGGCATAACTCTACCCTAAAGATC
  CATCAGAGGGTTCACAGTGGAGAGAAGCCTTACAAATGCAGTGAGTGTGGGAAGGCCTTCCACCGGCACA
  CTCACCTTAATGAACATCGGCGAATTCATACAGGCTACAGACCCCACAAATGTCAGGAATGCGTCAGGAG
  TTTCAGCCGGCCCTCACATCTGATGCGACATCAGGCCATTCACACCGCAGAAAAGCCCTATAGCTGTGCT
  GAATGCAAGGAGACTTTCAGCGATAACAATCGCCTTGTGCAACACCAGAAAATGCACACTGTCAAAACCC
  CATATGAATGTCAGGAGTGCGGAGAACGCTTCATTTGCGGCTCAACCCTGAAGTGCCACGAGAGTGTTCA
  CGCCAGAGAAAAACAAGGATTTTTTGTGAGTGGGAAGATCTTGGATCAGAACCCAGAACAGAAAGAGAAG
  TGCTTTAAGTGTAACAAATGTGAGAAAACCTTTAGCTGCAGCAAATACCTAACTCAGCACGAGAGGATTC
  ACACCAGGGGAGTGAAGCCCTTTGAATGTGACCAGTGTGGGAAAGCCTTTGGCCAAAGTACTCGGCTCAT
  TCACCATCAAAGAATCCACTCTAGAGTGAGGCTGTATAAATGGGGTGAGCAAGGGAAAGCCATCAGCAGT
  GCCTCCCTTATCAAACTTCAGTCCTTCCACACAAAGGAGCACCCTTTTAAATGTAACGAATGCGGAAAGA
  CCTTCAGCCACAGTGCACACCTCTCAAAACATCAGTTAATTCACGCTGGAGAGAATCCCTTTAAATGTAG
  TAAGTGTGACAGAGTCTTCACCCAGAGAAACTACCTTGTTCAGCATGAGCGAACTCATGCCAGAAAGAAG
  CCGTTGGTGTGTAACGAATGCGGGAAAACGTTCCGTCAGAGCTCATGCCTTTCTAAGCATCAGAGAATTC
  ACTCAGGTGAGAAGCCCTATGTATGTGATTACTGCGGGAAGGCCTTCGGCCTGAGTGCTGAGCTTGTCCG
  CCACCAGAGAATTCACACTGGAGAAAAGCCTTATGTTTGTCAGGAATGCGGGAAAGCCTTCACCCAGAGC
  TCATGCCTTTCTATTCACCGGAGAGTTCACACTGGGGAGAAGCCCTACAGATGTGGTGAATGTGGGAAAG
  CCTTTGCCCAGAAAGCAAATCTAACACAGCACCAGAGAATTCACACAGGGGAGAAGCCTTACTCCTGTAA
  TGTGTGTGGCAAAGCTTTTGTCCTCAGTGCCCATCTCAACCAGCACCTGAGAGTTCACACCCAGGAGACA
  CTTTATCAGTGTCAACGTTGCCAGAAAGCCTTTCGGTGCCACTCGAGCCTCAGCCGCCATCAGCGTGTAC
  ACAACAAGCAGCAATACTGCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212318 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEEFVTLKDVGMDFTLGDWEQLGLEQGDTFWDTALDNCQDLFLLDPPRPNLTSHPDGSEDLEPLAGGSP
  EATSPDVTETKNSPLMEDFFEEGFSQEIIEMLSKDGFWNSNFGEACIEDTWLDSLLGDPESLLRSDIATN
  GESPTECKSHELKRGLSPVSTVSTGEDSMVHNVSEKTLTPAKSKEYRGEFFSYSDHSQQDSVQEGEKPYQ
  CSECGKSFSGSYRLTQHWITHTREKPTVHQECEQGFDRNASLSVYPKTHTGYKFYVCNEYGTTFSQSTYL
  WHQKTHTGEKPCKSQDSDHPPSHDTQPGEHQKTHTDSKSYNCNECGKAFTRIFHLTRHQKIHTRKRYECS
  KCQATFNLRKHLIQHQKTHAAKTTSECQECGKIFRHSSLLIEHQALHAGEEPYKCNERGKSFRHNSTLKI
  HQRVHSGEKPYKCSECGKAFHRHTHLNEHRRIHTGYRPHKCQECVRSFSRPSHLMRHQAIHTAEKPYSCA
  ECKETFSDNNRLVQHQKMHTVKTPYECQECGERFICGSTLKCHESVHAREKQGFFVSGKILDQNPEQKEK
  CFKCNKCEKTFSCSKYLTQHERIHTRGVKPFECDQCGKAFGQSTRLIHHQRIHSRVRLYKWGEQGKAISS
  ASLIKLQSFHTKEHPFKCNECGKTFSHSAHLSKHQLIHAGENPFKCSKCDRVFTQRNYLVQHERTHARKK
  PLVCNECGKTFRQSSCLSKHQRIHSGEKPYVCDYCGKAFGLSAELVRHQRIHTGEKPYVCQECGKAFTQS
  SCLSIHRRVHTGEKPYRCGECGKAFAQKANLTQHQRIHTGEKPYSCNVCGKAFVLSAHLNQHLRVHTQET
  LYQCQRCQKAFRCHSSLSRHQRVHNKQQYCL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015428
• ORF Size: 2613 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015428.4
• RefSeq Size: 4711 bp
• RefSeq ORF: 2616 bp
• Locus ID: 25888
• UniProt ID: Q8WTR7
• Cytogenetics: 19q13.33
• MW: 100.2 kDa
• Gene Summary: This gene encodes a member of the Krueppel C2H2-type zinc-finger family of proteins. The encoded protein, a component of the U7 snRNP complex, plays a role in histone 3'-end pre-mRNA processing and may be required for cell cycle progression to S phase. Expression level and methylation status of this gene may be correlated with bone mineral density. [provided by RefSeq, Jul 2016]