PYHIN1 (NM_198930) Human Tagged ORF Clone

CAT#: RC212276

PYHIN1 (Myc-DDK-tagged)-Human pyrin and HIN domain family, member 1 (PYHIN1), transcript variant b2

Product Images

Circular map for RC212276

• 

  Western blot validation of overexpression lysate (Cat# LY404652) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212276 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PYHIN1
• Synonyms: IFIX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212276 representing NM_198930
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAATAACTACAAGAAAATTGTTCTACTGAAAGGATTAGAGGTCATCAATGATTATCATTTTAGAA
  TTGTTAAGTCCTTACTGAGTAACGATTTAAAACTTAATCCAAAAATGAAAGAAGAGTATGACAAAATTCA
  GATTGCTGACTTGATGGAGGAAAAGTTCCCAGGTGATGCCGGTTTGGGCAAACTAATAGAATTCTTCAAA
  GAAATACCAACACTGGGAGACCTTGCTGAAACTCTTAAAAGAGAAAAGTTAAAAGTCAAAGGAATAATCC
  CATCTAAAAAGACGAAACAGAAAGAAGTGTATCCTGCTACACCTGCATGCACCCCAAGCAACCGTCTCAC
  AGCTAAAGGAGCAGAGGAGACTCTTGGACCTCAGAAAAGAAAAAAACCATCTGAAGAAGAGACTGGAACC
  AAAAGGAGTAAGATGTCCAAAGAGCAGACTCGGCCTTCCTGCTCTGCAGGAGCCAGCACGTCCACAGCCA
  TGGGCCGTTCCCCACCTCCCCAGACCTCATCATCAGCTCCACCCAACACTTCCTCAACTGAGAGCCTAAA
  ACCATTGGCCAACCGTCACGCAACTGCCAGTAAAAATATTTTCCGAGAAGACCCAATAATCGCGATGGTA
  CTAAATGCAACAAAAGTATTTAAATATGAATCCTCAGAAAATGAGCAAAGAAGAATGTTTCATGCTACAG
  TGGCTACGCAGACACAGTTCTTTCATGTGAAGGTTTTAAACATCAACTTGAAGAGGAAATTCATTAAAAA
  GAGAATCATCATTATATCAAATTATTCCAAACGTAATAGTCTCCTAGAGGTGAATGAAGCCTCTTCTGTA
  TCTGAAGCTGGTCCTGACCAAACGTTTGAGGTTCCAAAGGACATCATCAGAAGAGCAAAGAAAATTCCGA
  AGATCAATATTCTTCACAAACAAACTTCAGGATATATTGTATATGGATTATTTATGCTACATACGAAAAT
  TGTAAATAGGAAGACGACAATCTATGAAATTCAGGATAAAACAGGAAGTATGGCTGTAGTAGGAAAAGGA
  GAATGCCACAATATCCCCTGTGAAAAAGGAGATAAGCTTCGACTCTTCTGCTTTCGACTGAGAAAGAGGG
  AAAATATGTCAAAACTGATGTCAGAAATGCATAGTTTCATCCAGATACAGAAAAATACAAACCAGAGAAG
  CCATGACTCCAGGAGCATGGCACTACCCCAGGAACAGAGTCAGCATCCAAAACCTTCAGAGGCCAGCACA
  ACCCTACCTGAAAGCCATCTCAAGACTCCTCAGATGCCACCAACAACCCCATCCAGCAGTTCCTTCACCA
  AGGTCACCAAGGACAAGGATATCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212276 representing NM_198930
  Red=Cloning site Green=Tags(s)
  
  MANNYKKIVLLKGLEVINDYHFRIVKSLLSNDLKLNPKMKEEYDKIQIADLMEEKFPGDAGLGKLIEFFK
  EIPTLGDLAETLKREKLKVKGIIPSKKTKQKEVYPATPACTPSNRLTAKGAEETLGPQKRKKPSEEETGT
  KRSKMSKEQTRPSCSAGASTSTAMGRSPPPQTSSSAPPNTSSTESLKPLANRHATASKNIFREDPIIAMV
  LNATKVFKYESSENEQRRMFHATVATQTQFFHVKVLNINLKRKFIKKRIIIISNYSKRNSLLEVNEASSV
  SEAGPDQTFEVPKDIIRRAKKIPKINILHKQTSGYIVYGLFMLHTKIVNRKTTIYEIQDKTGSMAVVGKG
  ECHNIPCEKGDKLRLFCFRLRKRENMSKLMSEMHSFIQIQKNTNQRSHDSRSMALPQEQSQHPKPSEAST
  TLPESHLKTPQMPPTTPSSSSFTKVTKDKDIK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198930
• ORF Size: 1356 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198930.4
• RefSeq Size: 1597 bp
• RefSeq ORF: 1359 bp
• Locus ID: 149628
• UniProt ID: Q6K0P9
• Cytogenetics: 1q23.1
• MW: 50.8 kDa
• Gene Summary: The protein encoded by this gene belongs to the HIN-200 family of interferon-inducible proteins that share a 200-amino acid signature motif at their C-termini. HIN200 proteins are primarily nuclear and are involved in transcriptional regulation of genes important for cell cycle control, differentiation, and apoptosis. Downregulation of this gene is associated with breast cancer. This protein acts as a tumor suppressor by promoting ubiquitination and subsequent degradation of MDM2, which leads to stabilization of p53/TP53. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]