SV2C (NM_014979) Human Tagged ORF Clone

CAT#: RC212245

SV2C (Myc-DDK-tagged)-Human synaptic vesicle glycoprotein 2C (SV2C)

Product Images

Circular map for RC212245

• 

  Western blot validation of overexpression lysate (Cat# LY414875) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212245 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SV2C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212245 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGACTCTTACAAGGATAGGACTTCACTGATGAAGGGTGCCAAGGACATTGCCAGAGAGGTGAAGA
  AACAAACAGTAAAGAAGGTGAATCAAGCTGTGGACCGAGCCCAGGATGAATACACCCAGAGGTCCTACAG
  TCGGTTCCAAGATGAAGAAGATGATGATGACTACTACCCGGCTGGAGAAACCTATAATGGTGAGGCCAAC
  GATGACGAAGGCTCAAGTGAAGCCACTGAGGGGCATGATGAAGATGATGAGATCTATGAGGGGGAGTATC
  AGGGCATCCCCAGTATGAACCAAGCGAAGGACAGCATCGTGTCAGTGGGGCAGCCCAAGGGCGATGAGTA
  CAAGGACCGACGGGAGCTGGAATCAGAAAGGAGAGCTGACGAGGAAGAGTTAGCCCAGCAGTATGAGCTG
  ATAATCCAAGAATGCGGTCATGGTCGTTTTCAGTGGGCCCTTTTCTTCGTCCTGGGCATGGCTCTTATGG
  CAGACGGTGTAGAGGTGTTTGTCGTTGGCTTCGTGTTACCCAGTGCTGAGACAGACCTCTGCATCCCAAA
  TTCAGGATCTGGATGGCTAGGCAGCATAGTGTACCTCGGGATGATGGTGGGGGCGTTCTTCTGGGGAGGA
  CTGGCAGACAAAGTGGGAAGGAAACAGTCTCTTCTGATTTGCATGTCTGTCAACGGATTCTTTGCCTTCC
  TTTCTTCATTTGTCCAAGGTTATGGCTTCTTTCTCTTCTGTCGCTTACTTTCTGGATTCGGGATTGGAGG
  AGCCATACCCACTGTGTTCTCGTACTTTGCTGAAGTCCTGGCCCGGGAAAAGCGGGGCGAACACTTGAGC
  TGGCTCTGCATGTTCTGGATGATCGGTGGCATCTACGCCTCTGCCATGGCCTGGGCCATCATCCCGCACT
  ACGGGTGGAGCTTCAGCATGGGATCGGCCTACCAGTTTCACAGTTGGCGTGTGTTTGTCATCGTCTGTGC
  ACTCCCCTGTGTCTCCTCCGTGGTGGCCCTCACATTCATGCCTGAAAGCCCACGATCCTTGTTGGAGGTT
  GGAAAACATGATGAAGCTTGGATGATTCTGAAGTTAATTCATGACACCAACATGAGAGCCCGGGGTCAGC
  CTGAGAAGGTCTTCACGGTAAACAAAATAAAAACTCCTAAACAAATAGATGAGCTGATTGAAATTGAGAG
  TGACACAGGAACATGGTATAGGAGGTGTTTTGTTCGGATCCGCACCGAGCTGTACGGAATTTGGTTGACT
  TTTATGAGATGTTTCAACTACCCAGTCAGGGATAATACAATAAAGCTTACAATTGTTTGGTTCACCCTGT
  CCTTTGGGTACTATGGATTATCCGTTTGGTTCCCTGATGTCATTAAACCTCTGCAGTCCGATGAATATGC
  ATTGCTAACCAGAAATGTGGAGAGAGATAAATATGCAAATTTCACTATTAACTTTACAATGGAAAATCAG
  ATTCATACTGGAATGGAATACGACAATGGCAGATTCATAGGGGTCAAGTTCAAATCTGTAACTTTCAAAG
  ACTCTGTTTTTAAGTCCTGCACCTTTGAGGATGTAACTTCAGTGAACACCTACTTCAAGAACTGCACATT
  TATTGACACTGTTTTTGACAACACAGATTTTGAGCCATATAAATTCATTGACAGTGAATTTAAAAACTGC
  TCGTTTTTTCACAACAAGACGGGATGTCAGATTACCTTTGATGATGACTATAGTGCCTACTGGATTTATT
  TTGTCAACTTTCTGGGGACATTGGCAGTATTGCCAGGGAACATTGTGTCTGCTCTGCTGATGGACAGAAT
  TGGGCGCTTAACAATGCTAGGTGGCTCTATGGTGCTTTCGGGGATCAGCTGTTTCTTCCTTTGGTTCGGC
  ACCAGTGAATCCATGATGATAGGCATGCTGTGTCTGTACAATGGATTGACCATCTCAGCCTGGAACTCTC
  TTGACGTGGTCACTGTGGAACTGTACCCCACAGACCGGAGGGCAACAGGCTTTGGCTTCTTAAATGCGCT
  ATGCAAGGCAGCAGCCGTCCTGGGAAACTTAATATTTGGCTCTCTGGTCAGCATCACCAAATCAATCCCC
  ATCCTGCTGGCTTCTACTGTGCTCGTGTGTGGAGGACTCGTTGGGCTGTGCCTGCCTGACACACGAACCC
  AGGTTCTGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212245 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEDSYKDRTSLMKGAKDIAREVKKQTVKKVNQAVDRAQDEYTQRSYSRFQDEEDDDDYYPAGETYNGEAN
  DDEGSSEATEGHDEDDEIYEGEYQGIPSMNQAKDSIVSVGQPKGDEYKDRRELESERRADEEELAQQYEL
  IIQECGHGRFQWALFFVLGMALMADGVEVFVVGFVLPSAETDLCIPNSGSGWLGSIVYLGMMVGAFFWGG
  LADKVGRKQSLLICMSVNGFFAFLSSFVQGYGFFLFCRLLSGFGIGGAIPTVFSYFAEVLAREKRGEHLS
  WLCMFWMIGGIYASAMAWAIIPHYGWSFSMGSAYQFHSWRVFVIVCALPCVSSVVALTFMPESPRSLLEV
  GKHDEAWMILKLIHDTNMRARGQPEKVFTVNKIKTPKQIDELIEIESDTGTWYRRCFVRIRTELYGIWLT
  FMRCFNYPVRDNTIKLTIVWFTLSFGYYGLSVWFPDVIKPLQSDEYALLTRNVERDKYANFTINFTMENQ
  IHTGMEYDNGRFIGVKFKSVTFKDSVFKSCTFEDVTSVNTYFKNCTFIDTVFDNTDFEPYKFIDSEFKNC
  SFFHNKTGCQITFDDDYSAYWIYFVNFLGTLAVLPGNIVSALLMDRIGRLTMLGGSMVLSGISCFFLWFG
  TSESMMIGMLCLYNGLTISAWNSLDVVTVELYPTDRRATGFGFLNALCKAAAVLGNLIFGSLVSITKSIP
  ILLASTVLVCGGLVGLCLPDTRTQVLM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014979
• ORF Size: 2181 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014979.4
• RefSeq Size: 10801 bp
• RefSeq ORF: 2184 bp
• Locus ID: 22987
• UniProt ID: Q496J9
• Cytogenetics: 5q13.3
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: ECM-receptor interaction
• MW: 82.3 kDa
• Gene Summary: Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles.[UniProtKB/Swiss-Prot Function]