GALNT3 (NM_004482) Human Tagged ORF Clone

CAT#: RC212219

GALNT3 (Myc-DDK-tagged)-Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 3 (GalNAc-T3) (GALNT3)

Product Images

Circular map for RC212219

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GALNT3
• Synonyms: GalNAc-T3; HFTC; HFTC1; HHS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212219 representing NM_004482
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCACCTAAAGCGACTAGTAAAATTACACATTAAAAGACATTACCATAAAAAGTTCTGGAAGCTTG
  GTGCAGTAATTTTTTTCTTTATAATAGTTTTGGTTTTAATGCAAAGAGAAGTAAGTGTTCAATATTCCAA
  AGAGGAATCAAGGATGGAAAGGAACATGAAAAACAAAAACAAGATGTTGGATTTAATGCTAGAAGCTGTA
  AACAATATTAAGGATGCCATGCCAAAAATGCAAATAGGAGCACCTGTCAGGCAAAACATTGATGCTGGTG
  AGAGACCTTGTTTGCAAGGATATTATACAGCAGCAGAATTGAAGCCTGTCCTTGACCGTCCACCTCAGGA
  TTCAAATGCACCTGGTGCTTCTGGTAAAGCATTCAAGACAACCAATTTAAGTGTTGAAGAGCAAAAGGAA
  AAGGAACGTGGGGAAGCTAAACACTGCTTTAATGCTTTCGCAAGTGACAGGATTTCTTTGCACCGAGATC
  TTGGACCAGACACTCGACCTCCTGAATGTATTGAACAAAAATTTAAGCGCTGCCCTCCCCTGCCCACCAC
  CAGTGTCATAATAGTTTTTCATAATGAAGCGTGGTCCACGTTGCTTAGAACTGTCCACAGTGTGCTCTAT
  TCTTCACCTGCAATACTGCTGAAGGAAATCATTTTGGTGGATGATGCTAGTGTAGATGAGTACTTACATG
  ATAAACTAGATGAATATGTAAAACAATTTTCTATAGTAAAAATAGTCAGACAAAGAGAAAGAAAAGGTCT
  GATCACTGCTCGGTTGCTAGGAGCAACAGTCGCAACAGCTGAAACGCTCACATTTTTAGATGCTCACTGT
  GAGTGTTTCTATGGTTGGCTAGAACCTCTGTTGGCCAGAATAGCTGAGAACTACACGGCTGTCGTAAGTC
  CAGATATTGCATCCATAGATCTGAACACGTTTGAATTCAACAAACCTTCTCCTTATGGAAGTAACCATAA
  CCGTGGAAATTTTGACTGGAGTCTTTCATTTGGCTGGGAGTCGCTTCCTGATCATGAGAAGCAAAGAAGG
  AAAGATGAAACCTACCCAATTAAAACACCCACTTTTGCAGGAGGACTTTTTTCCATATCAAAAGAATATT
  TTGAGTATATTGGAAGCTATGATGAAGAAATGGAAATCTGGGGAGGTGAAAATATAGAAATGTCTTTCAG
  AGTATGGCAATGTGGTGGGCAGTTGGAGATTATGCCTTGCTCTGTTGTTGGACATGTTTTTCGCAGCAAA
  AGCCCTCATAGCTTTCCAAAAGGCACTCAGGTGATTGCTAGAAACCAAGTTCGCCTTGCAGAAGTCTGGA
  TGGATGAATACAAGGAAATATTTTATAGGAGAAATACAGATGCAGCAAAAATTGTTAAACAAAAAGCATT
  TGGTGATCTTTCAAAAAGATTTGAAATAAAACACCGCCTTCAGTGTAAAAATTTTACATGGTATCTGAAC
  AACATTTATCCAGAGGTGTATGTGCCAGACCTTAATCCTGTTATATCTGGATACATTAAAAGCGTTGGTC
  AGCCTCTATGTCTGGATGTTGGAGAAAACAATCAAGGAGGCAAACCATTAATTATGTATACATGTCATGG
  ACTTGGGGGAAACCAGTACTTTGAATACTCTGCTCAACATGAAATTCGGCACAACATCCAGAAGGAATTA
  TGTCTTCATGCTGCTCAAGGTCTCGTTCAGCTGAAGGCATGTACCTACAAAGGTCACAAGACAGTTGTCA
  CTGGAGAGCAGATATGGGAGATCCAGAAGGATCAACTTCTATACAATCCATTCTTAAAAATGTGCCTTTC
  AGCAAATGGAGAGCATCCAAGTTTAGTGTCATGCAACCCATCAGATCCACTCCAAAAATGGATACTTAGC
  CAAAATGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212219 representing NM_004482
  Red=Cloning site Green=Tags(s)
  
  MAHLKRLVKLHIKRHYHKKFWKLGAVIFFFIIVLVLMQREVSVQYSKEESRMERNMKNKNKMLDLMLEAV
  NNIKDAMPKMQIGAPVRQNIDAGERPCLQGYYTAAELKPVLDRPPQDSNAPGASGKAFKTTNLSVEEQKE
  KERGEAKHCFNAFASDRISLHRDLGPDTRPPECIEQKFKRCPPLPTTSVIIVFHNEAWSTLLRTVHSVLY
  SSPAILLKEIILVDDASVDEYLHDKLDEYVKQFSIVKIVRQRERKGLITARLLGATVATAETLTFLDAHC
  ECFYGWLEPLLARIAENYTAVVSPDIASIDLNTFEFNKPSPYGSNHNRGNFDWSLSFGWESLPDHEKQRR
  KDETYPIKTPTFAGGLFSISKEYFEYIGSYDEEMEIWGGENIEMSFRVWQCGGQLEIMPCSVVGHVFRSK
  SPHSFPKGTQVIARNQVRLAEVWMDEYKEIFYRRNTDAAKIVKQKAFGDLSKRFEIKHRLQCKNFTWYLN
  NIYPEVYVPDLNPVISGYIKSVGQPLCLDVGENNQGGKPLIMYTCHGLGGNQYFEYSAQHEIRHNIQKEL
  CLHAAQGLVQLKACTYKGHKTVVTGEQIWEIQKDQLLYNPFLKMCLSANGEHPSLVSCNPSDPLQKWILS
  QND
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004482
• ORF Size: 1899 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004482.4
• RefSeq Size: 3874 bp
• RefSeq ORF: 1902 bp
• Locus ID: 2591
• UniProt ID: Q14435
• Cytogenetics: 2q24.3
• Domains: RICIN, Glycos_transf_2
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis
• MW: 72.4 kDa
• Gene Summary: This gene encodes UDP-GalNAc transferase 3, a member of the GalNAc-transferases family. This family transfers an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. Individual GalNAc-transferases have distinct activities and initiation of O-glycosylation is regulated by a repertoire of GalNAc-transferases. The protein encoded by this gene is highly homologous to other family members, however the enzymes have different substrate specificities. [provided by RefSeq, Jul 2008]