QTRT1 (NM_031209) Human Tagged ORF Clone

CAT#: RC212190

QTRT1 (Myc-DDK-tagged)-Human queuine tRNA-ribosyltransferase 1 (QTRT1)

Product Images

Circular map for RC212190

• 

  Western blot validation of overexpression lysate (Cat# LY410589) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212190 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: QTRT1
• Synonyms: FP3235; TGT; TGUT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212190 representing NM_031209
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGAGCAGCTACCCAGGCTTCCCTGGAGTCGGCCCCACGGATCATGCGGCTGGTGGCCGAATGCA
  GCCGCTCCAGGGCCCGGGCAGGCGAGCTGTGGCTGCCGCATGGGACAGTGGCCACTCCTGTGTTCATGCC
  AGTGGGCACGCAGGCCACCATGAAGGGCATCACGACCGAACAGCTGGACGCTCTGGGTTGCCGCATCTGC
  CTGGGCAATACCTACCATCTGGGTCTAAGGCCGGGACCCGAGCTGATCCAGAAAGCCAACGGTCTCCACG
  GCTTCATGAATTGGCCTCATAATCTGCTAACGGACAGCGGCGGTTTCCAGATGGTGTCGCTGGTGTCTCT
  GTCCGAGGTGACGGAGGAGGGCGTCCGCTTCCGCTCCCCCTACGACGGCAATGAGACCCTGCTGAGCCCG
  GAGAAATCCGTGCAGATCCAGAATGCGCTGGGCTCGGACATCATCATGCAGCTGGACGACGTGGTTAGCA
  GTACTGTGACTGGGCCACGTGTGGAGGAGGCCATGTACAGGTCAATCCGCTGGCTGGACCGGTGCATTGC
  AGCCCATCAGCGGCCGGACAAGCAGAACCTCTTCGCCATTATCCAGGGTGGGCTGGACGCAGATCTCCGG
  GCCACCTGCCTTGAAGAGATGACCAAGCGAGACGTGCCTGGCTTCGCCATCGGGGGCCTGAGCGGGGGTG
  AGAGCAAGTCGCAGTTCTGGCGGATGGTGGCGCTGAGCACCTCTCGGCTGCCGAAGGACAAGCCCCGATA
  TCTGATGGGGGTTGGCTATGCCACTGATCTGGTAGTCTGCGTGGCTCTTGGATGTGACATGTTCGACTGC
  GTCTTCCCCACACGGACAGCGCGCTTTGGCTCTGCCCTGGTGCCCACTGGGAACCTGCAGTTGAGGAAGA
  AGGTGTTTGAGAAGGACTTCGGCCCCATAGACCCGGAGTGCACCTGCCCCACGTGCCAAAAGCACAGCCG
  CGCCTTCCTGCACGCACTGCTGCACAGTGACAACACGGCCGCGCTGCACCACCTCACGGTCCACAACATC
  GCCTACCAGCTGCAGCTCATGAGCGCCGTCCGCACCAGCATCGTGGAGAAGCGCTTCCCGGACTTCGTGC
  GGGACTTCATGGGCGCCATGTACGGGGATCCCACCCTCTGTCCCACCTGGGCCACTGACGCTCTGGCCTC
  TGTGGGAATCACACTTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212190 representing NM_031209
  Red=Cloning site Green=Tags(s)
  
  MAGAATQASLESAPRIMRLVAECSRSRARAGELWLPHGTVATPVFMPVGTQATMKGITTEQLDALGCRIC
  LGNTYHLGLRPGPELIQKANGLHGFMNWPHNLLTDSGGFQMVSLVSLSEVTEEGVRFRSPYDGNETLLSP
  EKSVQIQNALGSDIIMQLDDVVSSTVTGPRVEEAMYRSIRWLDRCIAAHQRPDKQNLFAIIQGGLDADLR
  ATCLEEMTKRDVPGFAIGGLSGGESKSQFWRMVALSTSRLPKDKPRYLMGVGYATDLVVCVALGCDMFDC
  VFPTRTARFGSALVPTGNLQLRKKVFEKDFGPIDPECTCPTCQKHSRAFLHALLHSDNTAALHHLTVHNI
  AYQLQLMSAVRTSIVEKRFPDFVRDFMGAMYGDPTLCPTWATDALASVGITLG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031209
• ORF Size: 1209 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031209.1, NP_112486.1
• RefSeq Size: 1313 bp
• RefSeq ORF: 1212 bp
• Locus ID: 81890
• UniProt ID: Q9BXR0
• Cytogenetics: 19p13.2
• Domains: TGT
• MW: 43.9 kDa
• Gene Summary: This gene encodes the catalytic subunit of tRNA-guanine transglycosylase. tRNA-guanine transglycosylase is a heterodimeric enzyme complex that plays a critical role in tRNA modification by synthesizing the 7-deazaguanosine queuosine, which is found in tRNAs that code for asparagine, aspartic acid, histidine and tyrosine. A pseudogene of this gene is located on the long arm of chromosome X. [provided by RefSeq, Feb 2012]