CRISP1 (NM_001131) Human Tagged ORF Clone
CAT#: RC212061
CRISP1 (Myc-DDK-tagged)-Human cysteine-rich secretory protein 1 (CRISP1), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
"NM_001131" in other vectors (6)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | CRISP1 |
Synonyms | AEGL1; ARP; CRISP-1; HEL-S-57; HSCRISP1D; HSCRISP1G; HUMARP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC212061 representing NM_001131
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAATTAAACACCTCTTGTTTTTGGTTGCTGCTGCTTGCTTACTGCCTATGTTGTCCATGAAAAAGA AATCAGCTAGAGACCAATTTAATAAGCTCGTCACCGACTTGCCAAATGTACAAGAAGAGATCGTTAATAT ACACAACGCCCTCAGGAGAAGAGTAGTTCCACCAGCCAGCAACATGCTGAAGATGAGTTGGAGTGAAGAG GCTGCACAAAATGCCAGAATTTTTTCAAAGTATTGTGATATGACAGAGAGCAACCCCCTTGAGAGGAGAC TTCCAAATACCTTTTGTGGAGAAAATATGCATATGACATCTTATCCTGTATCATGGTCAAGTGTAATTGG AGTCTGGTACAGTGAGTCTACAAGTTTCAAACATGGAGAATGGACAACAACGGATGATGACATAACTACT GACCACTACACTCAGATTGTTTGGGCCACATCTTACCTGATTGGCTGTGCCATTGCATCTTGCCGCCAAC AAGGATCACCTCGATATCTCTACGTTTGTCACTATTGTCATGAGGGAAATGATCCTGAAACAAAGAATGA ACCTTATAAGACAGGCGTCCCATGTGAAGCCTGCCCAAGTAACTGTGAAGACAAACTTTGCACTAACCCC TGCATCTACTATGATGAATACTTCGACTGTGACATACAAGTCCATTATCTGGGATGCAACCACTCAACAA CTATCCTATTCTGTAAAGCCACTTGTCTGTGTGACACTGAGATAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212061 representing NM_001131
Red=Cloning site Green=Tags(s) MEIKHLLFLVAAACLLPMLSMKKKSARDQFNKLVTDLPNVQEEIVNIHNALRRRVVPPASNMLKMSWSEE AAQNARIFSKYCDMTESNPLERRLPNTFCGENMHMTSYPVSWSSVIGVWYSESTSFKHGEWTTTDDDITT DHYTQIVWATSYLIGCAIASCRQQGSPRYLYVCHYCHEGNDPETKNEPYKTGVPCEACPSNCEDKLCTNP CIYYDEYFDCDIQVHYLGCNHSTTILFCKATCLCDTEIK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001131 |
ORF Size | 747 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001131.3 |
RefSeq Size | 1892 bp |
RefSeq ORF | 750 bp |
Locus ID | 167 |
UniProt ID | P54107 |
Cytogenetics | 6p12.3 |
MW | 28.48 kDa |
Gene Summary | Fertilization consists of a sequence of specific cell-cell interactions culminating in the fusion of the sperm and egg plasma membranes. Recognition, binding, and fusion occur through the interaction of complementary molecules that are localized to specific domains of the sperm and egg plasma membranes. In the sperm, the postacrosomal region or equatorial segment is involved in sperm-egg plasma membrane fusion. The protein encoded by this gene is a member of the cysteine-rich secretory protein (CRISP) family. It is expressed in the epididymis, is secreted into the epididymal lumen, and binds to the postacrosomal region of the sperm head, where it plays a role in sperm-egg fusion. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC212061L1 | Lenti ORF clone of Human cysteine-rich secretory protein 1 (CRISP1), transcript variant 1, Myc-DDK-tagged |
USD 600.00 |
|
RC212061L2 | Lenti ORF clone of Human cysteine-rich secretory protein 1 (CRISP1), transcript variant 1, mGFP tagged |
USD 600.00 |
|
RC212061L3 | Lenti ORF clone of Human cysteine-rich secretory protein 1 (CRISP1), transcript variant 1, Myc-DDK-tagged |
USD 600.00 |
|
RC212061L4 | Lenti ORF clone of Human cysteine-rich secretory protein 1 (CRISP1), transcript variant 1, mGFP tagged |
USD 600.00 |
|
RG212061 | CRISP1 (tGFP-tagged) - Human cysteine-rich secretory protein 1 (CRISP1), transcript variant 1 |
USD 500.00 |
|
SC302975 | CRISP1 (untagged)-Human cysteine-rich secretory protein 1 (CRISP1), transcript variant 1 |
USD 300.00 |
{0} Product Review(s)
Be the first one to submit a review