Aurora A (AURKA) (NM_198433) Human Tagged ORF Clone
AURKA (Myc-DDK-tagged)-Human aurora kinase A (AURKA), transcript variant 1
|Product Name||Aurora A (AURKA) (NM_198433) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||AIK; ARK1; AURA; BTAK; PPP1R47; STK6; STK7; STK15|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC212018 representing NM_198433
Red=Cloning site Blue=ORF Green=Tags(s)
>RC212018 representing NM_198433
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1209 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_198433.1, NM_198433.2, NP_940835.1|
|RefSeq Size||2554 bp|
|RefSeq ORF||1212 bp|
|Protein Families||Druggable Genome, Protein Kinase, Stem cell - Pluripotency|
|Protein Pathways||Oocyte meiosis|
|Gene Summary||The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]|
|The use of this cDNA Clones has been cited in the following citations:|
MiR-26a-5p Inhibits Cell Proliferation and Enhances Doxorubicin Sensitivity in HCC Cells via Targeting AURKA
,Yuan, YL;Yu, H;Mu, SM;Dong, YD;Li, Y;,
Technol. Cancer Res. Treat. 2019
,PubMed ID 31570091
Opposing roles for JNK and Aurora A in regulating WD40-Repeat Protein 62 association with spindle microtubules
,Lim, NR;Yeap, YY;Zhao, TT;Yip, YY;Wong, SC;Xu, D;Ang, CS;Williamson, NA;Xu, Z;Bogoyevitch, MA;Ng, DC;,
J. Cell. Sci. Dec 2014
,PubMed ID 25501809
|cDNA Clone Resources|
|SC107819||AURKA (untagged)-Human aurora kinase A (AURKA), transcript variant 1||
|RG212018||AURKA (GFP-tagged) - Human aurora kinase A (AURKA), transcript variant 1||
|RC212018L1||Lenti ORF clone of Human aurora kinase A (AURKA), transcript variant 1, Myc-DDK-tagged||
|RC212018L2||Lenti ORF clone of Human aurora kinase A (AURKA), transcript variant 1, mGFP tagged||
|RC212018L3||Lenti ORF clone of Human aurora kinase A (AURKA), transcript variant 1, Myc-DDK-tagged||
|RC212018L4||Lenti ORF clone of Human aurora kinase A (AURKA), transcript variant 1, mGFP tagged||