ARNT2 (NM_014862) Human Tagged ORF Clone

CAT#: RC211966

ARNT2 (Myc-DDK-tagged)-Human aryl-hydrocarbon receptor nuclear translocator 2 (ARNT2)

Product Images

Circular map for RC211966

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  Western blot validation of overexpression lysate (Cat# LY402381) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211966 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ARNT2 protein (Cat# TP311966). The protein was produced from HEK293T cells transfected with ARNT2 cDNA clone (Cat# RC211966) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARNT2
• Synonyms: bHLHe1; WEDAS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211966 representing NM_014862
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAACCCCGGCGGCGGTCAACCCTCCGGAAATGGCTTCAGACATACCTGGATCTGTGACGTTGCCCG
  TTGCCCCCATGGCGGCCACCGGACAGGTGAGGATGGCGGGGGCCATGCCTGCCCGTGGAGGAAAGCGGCG
  TTCCGGAATGGACTTCGATGATGAAGATGGTGAAGGCCCCAGTAAATTTTCAAGAGAGAATCATAGTGAA
  ATCGAAAGGCGCAGACGGAACAAGATGACTCAGTACATCACGGAGCTCTCCGACATGGTCCCCACATGCA
  GCGCACTGGCTCGGAAGCCAGACAAGCTCACCATCCTCCGCATGGCCGTCTCGCACATGAAGTCCATGAG
  GGGTACAGGGAACAAGTCCACCGATGGCGCGTACAAGCCTTCCTTCCTCACAGAGCAGGAACTGAAGCAT
  CTCATCCTTGAAGCAGCTGATGGATTTCTGTTTGTGGTGGCTGCTGAGACAGGGCGAGTGATTTATGTGT
  CTGACTCCGTCACCCCTGTTCTGAACCAGCCCCAGTCAGAGTGGTTTGGGAGCACACTGTATGAACAGGT
  GCATCCTGATGACGTGGAGAAGCTGAGAGAGCAACTGTGCACCTCAGAAAACTCAATGACAGGCCGGATC
  TTGGACCTGAAGACTGGGACGGTCAAGAAAGAAGGGCAGCAGTCATCCATGAGGATGTGCATGGGCTCGC
  GGCGGTCTTTCATCTGCAGGATGAGGTGTGGAAATGCTCCTTTGGACCACCTTCCTCTAAACAGAATAAC
  CACCATGAGGAAAAGGTTCAGGAATGGCCTTGGCCCTGTGAAAGAAGGAGAAGCCCAATATGCTGTGGTC
  CACTGTACAGGATACATCAAGGCCTGGCCACCAGCAGGAATGACCATACCTGAAGAAGACGCTGATGTGG
  GACAAGGCAGTAAATATTGCCTCGTGGCAATTGGGAGACTCCAGGTGACCAGCTCTCCTGTATGCATGGA
  CATGAATGGGATGTCGGTGCCCACAGAGTTCTTATCCCGGCATAACTCCGATGGAATCATCACATTTGTG
  GATCCAAGATGTATCAGTGTGATTGGCTACCAACCCCAGGATCTTCTGGGAAAGGACATTTTGGAATTCT
  GCCACCCTGAGGATCAAAGCCATCTGCGTGAGAGCTTCCAGCAGGTGGTTAAGCTGAAAGGCCAAGTCCT
  GTCGGTCATGTATCGATTTCGCACCAAGAACCGGGAGTGGATGTTGATCCGCACCAGCAGCTTCACATTC
  CAGAATCCCTATTCTGATGAGATTGAGTACATCATCTGCACCAACACCAACGTCAAGCAACTTCAGCAAC
  AGCAGGCAGAATTGGAAGTGCACCAGAGAGATGGATTGTCATCGTATGACTTATCCCAGGTCCCCGTCCC
  CAACCTACCAGCCGGTGTTCATGAGGCCGGGAAGTCCGTGGAAAAGGCGGATGCAATCTTCTCCCAGGAA
  AGAGATCCTCGGTTTGCTGAAATGTTTGCAGGAATTAGTGCATCGGAGAAGAAGATGATGAGCTCAGCCT
  CTGCAGCAGGAACCCAGCAGATCTACTCCCAAGGAAGCCCATTTCCCTCTGGACACTCCGGGAAGGCCTT
  CAGCTCTTCAGTGGTTCATGTGCCTGGAGTGAATGATATTCAGTCCTCTTCTTCCACGGGCCAGAACATG
  TCCCAAATCTCCCGGCAGCTAAACCAGAGTCAGGTGGCATGGACAGGGAGTCGTCCGCCCTTTCCGGGAC
  AGCAAATCCCATCTCAGTCCAGCAAGACTCAGTCATCTCCCTTTGGGATTGGAACGAGCCACACCTACCC
  GGCAGACCCCTCTTCCTACAGCCCCCTCTCCAGCCCAGCTACCTCCTCGCCAAGTGGGAATGCCTACTCC
  AGTCTTGCCAACAGGACTCCAGGGTTCGCTGAAAGTGGACAAAGTAGCGGGCAGTTCCAAGGGCGGCCCT
  CGGAAGTCTGGTCGCAGTGGCAAAGCCAGCACCATGGCCAGCAGAGCGGTGAGCAGCACTCCCACCAGCA
  GCCCGGTCAGACTGAAGTGTTCCAGGACATGCTGCCCATGCCAGGAGATCCAACCCAGGGGACTGGCAAC
  TATAACATCGAAGACTTTGCCGACCTGGGCATGTTTCCACCGTTTTCTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211966 representing NM_014862
  Red=Cloning site Green=Tags(s)
  
  MATPAAVNPPEMASDIPGSVTLPVAPMAATGQVRMAGAMPARGGKRRSGMDFDDEDGEGPSKFSRENHSE
  IERRRRNKMTQYITELSDMVPTCSALARKPDKLTILRMAVSHMKSMRGTGNKSTDGAYKPSFLTEQELKH
  LILEAADGFLFVVAAETGRVIYVSDSVTPVLNQPQSEWFGSTLYEQVHPDDVEKLREQLCTSENSMTGRI
  LDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGNAPLDHLPLNRITTMRKRFRNGLGPVKEGEAQYAVV
  HCTGYIKAWPPAGMTIPEEDADVGQGSKYCLVAIGRLQVTSSPVCMDMNGMSVPTEFLSRHNSDGIITFV
  DPRCISVIGYQPQDLLGKDILEFCHPEDQSHLRESFQQVVKLKGQVLSVMYRFRTKNREWMLIRTSSFTF
  QNPYSDEIEYIICTNTNVKQLQQQQAELEVHQRDGLSSYDLSQVPVPNLPAGVHEAGKSVEKADAIFSQE
  RDPRFAEMFAGISASEKKMMSSASAAGTQQIYSQGSPFPSGHSGKAFSSSVVHVPGVNDIQSSSSTGQNM
  SQISRQLNQSQVAWTGSRPPFPGQQIPSQSSKTQSSPFGIGTSHTYPADPSSYSPLSSPATSSPSGNAYS
  SLANRTPGFAESGQSSGQFQGRPSEVWSQWQSQHHGQQSGEQHSHQQPGQTEVFQDMLPMPGDPTQGTGN
  YNIEDFADLGMFPPFSE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014862
• ORF Size: 2151 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014862.3, NP_055677.3
• RefSeq Size: 6576 bp
• RefSeq ORF: 2154 bp
• Locus ID: 9915
• UniProt ID: Q9HBZ2
• Cytogenetics: 15q25.1
• Domains: PAS, HLH, PAC
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma
• MW: 78.5 kDa
• Gene Summary: This gene encodes a member of the basic-helix-loop-helix-Per-Arnt-Sim (bHLH-PAS) superfamily of transcription factors. The encoded protein acts as a partner for several sensor proteins of the bHLH-PAS family, forming heterodimers with the sensor proteins that bind regulatory DNA sequences in genes responsive to developmental and environmental stimuli. Under hypoxic conditions, the encoded protein complexes with hypoxia-inducible factor 1alpha in the nucleus and this complex binds to hypoxia-responsive elements in enhancers and promoters of oxygen-responsive genes. A highly similar protein in mouse forms functional complexes with both aryl hydrocarbon receptors and Single-minded proteins, suggesting additional roles for the encoded protein in the metabolism of xenobiotic compounds and the regulation of neurogenesis, respectively. [provided by RefSeq, Dec 2013]