SPATA5 (NM_145207) Human Tagged ORF Clone

CAT#: RC211834

SPATA5 (Myc-DDK-tagged)-Human spermatogenesis associated 5 (SPATA5)

Product Images

Circular map for RC211834

• 

  Coomassie blue staining of purified SPATA5 protein (Cat# TP311834). The protein was produced from HEK293T cells transfected with SPATA5 cDNA clone (Cat# RC211834) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SPATA5
• Synonyms: AFG2; EHLMRS; SPAF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211834 representing NM_145207
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTCCAAGAAGAATAGAAAGCGGTTGAACCAAAGCGCGGAAAATGGTTCGTCCTTGCCCTCTGCTG
  CTTCCTCTTGTGCGGAGGCACGGGCTCCTTCTGCTGGATCAGACTTCGCGGCAACCTCCGGGACTCTGAC
  GGTGACCAACTTATTAGAAAAGGTAGATGACAAAATTCCTAAAACATTCCAGAATTCCCTTATTCATCTT
  GGACTCAACACTATGAAGTCTGCAAATATATGTATAGGTCGACCAGTGTTGCTTACTAGTTTGAACGGAA
  AGCAAGAGGTGTATACAGCCTGGCCTATGGCAGGATTTCCTGGAGGCAAGGTCGGCCTGAGTGAAATGGC
  ACAGAAAAATGTGGGTGTGAGGCCTGGTGATGCCATCCAGGTCCAGCCTCTTGTGGGTGCTGTGCTACAG
  GCTGAGGAAATGGATGTGGCACTGAGTGACAAAGATATGGAAATTAATGAAGAAGAACTGACTGGTTGTA
  TCCTGAGAAAACTAGATGGCAAGATTGTTTTACCAGGCAACTTTCTGTATTGTACATTCTATGGACGACC
  GTACAAGCTGCAAGTATTGCGAGTGAAAGGGGCAGATGGCATGATATTGGGAGGGCCTCAGAGTGACTCT
  GACACTGATGCCCAAAGAATGGCCTTTGAACAGTCCAGCATGGAAACCAGTAGCCTGGAGTTATCCTTAC
  AGCTAAGCCAGTTAGATCTGGAGGATACCCAGATCCCAACATCAAGAAGTACTCCTTATAAACCAATTGA
  TGACAGAATTACAAATAAAGCCAGTGATGTTTTGCTGGATGTTACACAGAGCCCTGGAGATGGCAGTGGA
  CTTATGCTAGAGGAAGTCACAGGTCTTAAATGTAATTTTGAATCTGCCAGAGAAGGAAATGAGCAACTTA
  CTGAAGAAGAGAGACTGCTAAAGTTCAGCATAGGAGCAAAGTGCAATACTGATACTTTTTATTTTATTTC
  TTCAACAACAAGAGTCAATTTTACAGAGATTGATAAAAATTCAAAAGAGCAAGACAACCAATTCAAAGTA
  ACTTATGACATGATAGGAGGATTAAGTAGCCAGCTGAAAGCAATTAGAGAAATAATTGAATTGCCCCTCA
  AACAGCCTGAGCTTTTCAAGAGTTATGGAATTCCTGCCCCTAGAGGAGTGTTACTTTATGGTCCTCCAGG
  TACTGGAAAAACAATGATCGCCAGGGCTGTTGCTAATGAAGTTGGAGCCTATGTTTCTGTAATTAATGGT
  CCTGAAATTATAAGCAAATTCTATGGTGAGACTGAAGCAAAGTTACGTCAGATATTTGCTGAAGCCACTC
  TACGACACCCATCAATTATTTTTATTGATGAGCTGGATGCACTTTGTCCGAAAAGAGAGGGGGCCCAGAA
  TGAAGTGGAAAAAAGAGTTGTGGCTTCACTCTTAACACTGATGGATGGCATTGGTTCAGAAGTAAGTGAA
  GGACAAGTGTTGGTTCTTGGGGCCACAAATCGCCCTCATGCCTTGGATGCTGCTCTCCGAAGACCTGGGC
  GATTTGATAAAGAGATTGAGATTGGAGTTCCCAATGCTCAGGACCGGCTAGATATTCTCCAGAAACTGCT
  TCGAAGGGTACCCCATTTGCTCACTGAGGCTGAGCTGCTGCAGCTGGCAAATAGTGCTCATGGATACGTT
  GGAGCAGACTTGAAAGTCTTGTGTAATGAAGCAGGTCTCTGTGCCTTGCGGAGAATCCTGAAAAAACAGC
  CTAACCTCCCTGATGTCAAGGTGGCTGGACTGGTGAAGATTACTCTGAAGGATTTCTTGCAGGCAATGAA
  TGATATCAGACCCAGTGCCATGAGGGAAATAGCAATTGATGTCCCAAATGTATCCTGGTCAGATATAGGA
  GGACTGGAAAGTATCAAACTGAAGTTGGAACAGGCTGTGGAATGGCCCTTAAAACATCCAGAGTCTTTCA
  TTCGAATGGGTATTCAGCCACCTAAAGGAGTTCTTCTCTATGGGCCACCTGGGTGCTCTAAAACAATGAT
  AGCAAAGGCTTTGGCCAATGAGAGTGGACTGAATTTTCTAGCTATAAAGGGGCCTGAATTAATGAATAAA
  TATGTTGGTGAATCTGAAAGAGCAGTTAGAGAGACCTTCCGAAAAGCAAGAGCAGTGGCGCCTTCCATTA
  TTTTCTTTGATGAACTGGATGCCTTAGCAGTTGAAAGGGGCAGTTCTTTAGGTGCTGGGAATGTAGCCGA
  TCGTGTTTTGGCTCAGCTCTTAACAGAAATGGATGGGATTGAACAGCTAAAGGATGTGACCATTTTGGCA
  GCTACTAACCGTCCAGATAGGATAGACAAGGCTTTGATGCGGCCTGGAAGAATTGATAGAATCATCTATG
  TGCCTTTACCGGATGCAGCAACAAGAAGGGAAATATTTAAGCTGCAGTTTCACTCCATGCCTGTCAGTAA
  TGAAGTTGACCTGGATGAACTCATCCTTCAAACCGACGCATACTCAGGAGCAGAGATTGTAGCTGTCTGC
  AGAGAGGCAGCTCTTCTGGCTCTGGAAGAAGACATTCAAGCCAATCTCATCATGAAAAGACATTTCACTC
  AGGCCTTGAGCACTGTGACACCTAGAATTCCTGAGTCATTGAGACGTTTTTATGAAGATTATCAAGAGAA
  GAGTGGGCTGCATACACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211834 representing NM_145207
  Red=Cloning site Green=Tags(s)
  
  MSSKKNRKRLNQSAENGSSLPSAASSCAEARAPSAGSDFAATSGTLTVTNLLEKVDDKIPKTFQNSLIHL
  GLNTMKSANICIGRPVLLTSLNGKQEVYTAWPMAGFPGGKVGLSEMAQKNVGVRPGDAIQVQPLVGAVLQ
  AEEMDVALSDKDMEINEEELTGCILRKLDGKIVLPGNFLYCTFYGRPYKLQVLRVKGADGMILGGPQSDS
  DTDAQRMAFEQSSMETSSLELSLQLSQLDLEDTQIPTSRSTPYKPIDDRITNKASDVLLDVTQSPGDGSG
  LMLEEVTGLKCNFESAREGNEQLTEEERLLKFSIGAKCNTDTFYFISSTTRVNFTEIDKNSKEQDNQFKV
  TYDMIGGLSSQLKAIREIIELPLKQPELFKSYGIPAPRGVLLYGPPGTGKTMIARAVANEVGAYVSVING
  PEIISKFYGETEAKLRQIFAEATLRHPSIIFIDELDALCPKREGAQNEVEKRVVASLLTLMDGIGSEVSE
  GQVLVLGATNRPHALDAALRRPGRFDKEIEIGVPNAQDRLDILQKLLRRVPHLLTEAELLQLANSAHGYV
  GADLKVLCNEAGLCALRRILKKQPNLPDVKVAGLVKITLKDFLQAMNDIRPSAMREIAIDVPNVSWSDIG
  GLESIKLKLEQAVEWPLKHPESFIRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAIKGPELMNK
  YVGESERAVRETFRKARAVAPSIIFFDELDALAVERGSSLGAGNVADRVLAQLLTEMDGIEQLKDVTILA
  ATNRPDRIDKALMRPGRIDRIIYVPLPDAATRREIFKLQFHSMPVSNEVDLDELILQTDAYSGAEIVAVC
  REAALLALEEDIQANLIMKRHFTQALSTVTPRIPESLRRFYEDYQEKSGLHTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145207
• ORF Size: 2679 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145207.3
• RefSeq Size: 3359 bp
• RefSeq ORF: 2682 bp
• Locus ID: 166378
• UniProt ID: Q8NB90
• Cytogenetics: 4q28.1
• Domains: AAA
• MW: 97.7 kDa
• Gene Summary: This gene encodes a member of the ATPase associated with diverse activities family, whose members are defined by a highly conserved ATPase domain. Members of this family participate in diverse cellular processes that include membrane fusion, DNA replication, microtubule severing, and protein degradation. The protein encoded by this gene has a putative mitochondrial targeting sequence and has been proposed to function in maintenance of mitochondrial function and integrity during mouse spermatogenesis. Allelic variants in this gene have been associated with epilepsy, hearing loss, and cognitive disability syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]