KCNG2 (NM_012283) Human Tagged ORF Clone

CAT#: RC211800

KCNG2 (Myc-DDK-tagged)-Human potassium voltage-gated channel, subfamily G, member 2 (KCNG2)

Product Images

Circular map for RC211800

• 

  Western blot validation of overexpression lysate (Cat# LY415851) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211800 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCNG2
• Synonyms: KCNF2; KV6.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211800 representing NM_012283.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGCCATGGCCCTGCTCCCCGGGCGGCGGCGGCGGGACCCGCGCCCGGCACGTCATCATCAACGTG
  GGCGGCTGCCGCGTGCGCCTGGCATGGGCCGCGCTGGCGCGATGCCCCCTCGCGCGCCTGGAGCGCCTG
  CGCGCCTGCCGTGGCCACGACGACCTGCTGCGCGTGTGTGACGACTACGACGTGAGCCGCGACGAGTTC
  TTCTTCGACCGCAGCCCGTGCGCCTTCCGCGCCATCGTGGCGCTTTTGCGCGCAGGGAAGCTGCGACTG
  CTGCGGGGCCCGTGCGCGCTGGCCTTCCGCGACGAGCTGGCCTACTGGGGCATCGACGAGGCGCGCCTG
  GAGCGCTGCTGCCTGCGCCGCCTGCGCCGCCGCGAGGAGGAGGCGGCCGAGGCCCGCGCGGGGCCGACG
  GAGCGCGGGGCGCAGGGGAGCCCGGCGCGCGCCCTGGGACCTCGGGGGCGGCTGCAGCGCGGCCGGCGG
  CGCCTGCGCGACGTGGTGGACAACCCGCACTCGGGGCTGGCGGGCAAGCTCTTCGCCTGCGTGTCCGTG
  TCCTTCGTGGCCGTCACGGCCGTGGGCCTCTGCCTGAGCACCATGCCGGACATCCGCGCCGAGGAGGAG
  CGGGGCGAGTGCTCCCCCAAGTGCCGCAGCCTGTTCGTGCTGGAGACCGTGTGCGTGGCCTGGTTCTCC
  TTCGAGTTCCTGCTGCGCTCCCTGCAGGCCGAGAGCAAGTGCGCCTTCCTGCGCGCGCCACTCAACATC
  ATTGACATCCTGGCGCTCCTGCCGTTCTACGTGTCGCCGCTGCTGGGGCTGGCGGCAGGCCCGGGCGGG
  ACCAAGCTCCTGGAGCGCGCGGGGCTGGTGCTGCGGCTGCTGCGTGCGCTGCGCGTGCTCTACGTGATG
  CGCCTGGCGCGCCACTCGCTGGGGCTGCGTTCGCTTGGCCTGACCATGCGCCGCTGCGCGCGCGAGTTC
  GGGCTGCTGCTGCTGTTCCTCTGCGTGGCCATGGCGCTCTTCGCGCCACTGGTGCACCTGGCCGAGCGC
  GAGCTGGGCGCGCGCCGCGACCTCTCCAGCGTGCCCGCCAGCTATTGGTGGGCCGTCATCTCCATGACC
  ACCGTGGGCTACGGCGACATGGTCCCGCGCAGCTTGCCCGGGCAGGTGGTGGCGCTCAGCAGCATCCTC
  AGCGGCATCCTGCTCATGGCCTTCCCGGTCACCTCCATCTTCCACACCTTTTCGCGCTCCTACTCCGAG
  CTCAAGGAGCAGCAGCAGCGCGCGGCCAGCCCCGAGCCGGCCCTGCAGGAGGACAGCACGCACTCGGCC
  ACAGCCACCGAGGACAGCTCGCAGGGCCCCGACAGCGCGGGCCTGGCCGACGACTCCGCGGATGCGCTG
  TGGGTGCGGGCAGGGCGC
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by RC211800
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MEPWPCSPGGGGGTRARHVIINVGGCRVRLAWAALARCPLARLERLRACRGHDDLLRVCDDYDVSRDEF
  FFDRSPCAFRAIVALLRAGKLRLLRGPCALAFRDELAYWGIDEARLERCCLRRLRRREEEAAEARAGPT
  ERGAQGSPARALGPRGRLQRGRRRLRDVVDNPHSGLAGKLFACVSVSFVAVTAVGLCLSTMPDIRAEEE
  RGECSPKCRSLFVLETVCVAWFSFEFLLRSLQAESKCAFLRAPLNIIDILALLPFYVSPLLGLAAGPGG
  TKLLERAGLVLRLLRALRVLYVMRLARHSLGLRSLGLTMRRCAREFGLLLLFLCVAMALFAPLVHLAER
  ELGARRDLSSVPASYWWAVISMTTVGYGDMVPRSLPGQVVALSSILSGILLMAFPVTSIFHTFSRSYSE
  LKEQQQRAASPEPALQEDSTHSATATEDSSQGPDSAGLADDSADALWVRAGR
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012283
• ORF Size: 1398 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 1401 bp
• RefSeq ORF: 1401 bp
• Locus ID: 26251
• UniProt ID: Q9UJ96
• Cytogenetics: 18q23
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• MW: 51.2 kDa
• Gene Summary: Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, subfamily G. This member is a gamma subunit of the voltage-gated potassium channel. The delayed-rectifier type channels containing this subunit may contribute to cardiac action potential repolarization. [provided by RefSeq, Jul 2008]