ARHGAP9 (NM_001080157) Human Tagged ORF Clone

CAT#: RC211794

ARHGAP9 (Myc-DDK-tagged)-Human Rho GTPase activating protein 9 (ARHGAP9), transcript variant 2

Product Images

Circular map for RC211794

• 

  Western blot validation of overexpression lysate (Cat# LY421604) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211794 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARHGAP9
• Synonyms: 10C; RGL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211794 representing NM_001080157
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTATCCAGCCGGTGGTGGCCAAGTTCCTGGGGGATCCTAGGGCTGGGCCCCCGAAGCCCTCCTCGGG
  GATCCCAGCTCTGTGCCCTCTATGCCTTTACTTATACTGGGGCAGATGGCCAGCAGGTGTCTCTGGCTGA
  AGGGGATAGGTTCCTACTGCTTCGAAAGACCAACTCCGACTGGTGGTTGGCAAGACGCCTAGAAGCTCCC
  TCCACCTCTCGACCCATCTTCGTCCCAGCAGCCTATATGATAGAGGAATCCATCCCTTCCCAGAGTCCAA
  CTACCGTCATCCCCGGCCAATTGCTCTGGACTCCTGGGCCGAAGTTGTTTCATGGTTCCCTGGAGGAGTT
  GTCTCAGGCCCTCCCAAGCAGGGCTCAGGCTAGCTCGGAGCAGCCTCCTCCACTTCCCCGCAAAATGTGT
  AGGAGCGTCAGCACTGACAATCTGAGCCCCAGCCTTCTGAAGCCTTTCCAGGAAGGACCAAGCGGAAGAT
  CCCTCTCCCAGGAAGACTTGCCGTCAGAAGCCAGTGCCAGCACAGCAGGCCCCCAGCCCCTCATGTCAGA
  GCCCCCTGTGTACTGTAACCTGGTGGACCTTCGCCGCTGTCCTCGGTCCCCACCCCCAGGCCCTGCATGC
  CCCCTGCTGCAGAGGCTGGATGCCTGGGAGCAGCACCTGGACCCCAACTCTGGACGCTGCTTCTACATAA
  ATTCACTGACTGGCTGCAAGTCCTGGAAGCCCCCGCGCCGCAGTCGCAGCGAGACGAACCCTGGCTCCAT
  GGAGGGGACACAGACCCTGAAGAGGAACAATGATGTCCTGCAACCTCAGGCAAAGGGCTTCAGATCTGAC
  ACAGGGACCCCAGAACCGCTTGACCCACAGGGTTCACTCAGCCTCAGCCAACGCACCTCGCAGCTTGACC
  CTCCAGCCTTGCAGGCCCCTCGACCTCTGCCGCAGCTCCTGGACGACCCCCATGAGGTGGAAAAGTCGGG
  TCTGCTCAACATGACCAAGATTGCCCAAGGGGGGCGCAAGCTCAGGAAGAACTGGGGCCCGTCTTGGGTG
  GTGTTAACGGGTAACAGCCTGGTGTTCTACCGAGAGCCACCGCCGACAGCGCCCTCCTCAGGCTGGGGAC
  CAGCGGGTAGCCGGCCCGAAAGTAGCGTGGACCTGCGCGGGGCGGCCCTGGCGCACGGCCGCCACCTGTC
  CAGCCGCCGCAACGTCCTGCACATCCGCACGATCCCTGGCCACGAGTTCCTGCTGCAGTCGGACCACGAG
  ACAGAGCTGCGAGCCTGGCACCGCGCGCTGCGGACTGTCATCGAGCGGCTGGATCGGGAGAACCCCCTGG
  AGCTGCGTCTGTCGGGCTCTGGACCCGCGGAGCTGAGCGCCGGGGAGGACGAAGAAGAGGAGTCGGAGCT
  GGTGTCCAAGCCGCTGCTGCGCCTCAGCAGCCGCCGGAGCTCCATTCGGGGGCCCGAAGGCACCGAGCAG
  AACCGCGTGCGCAACAAACTAAAGCGGCTCATCGCGAAGAGACCGCCCTTACAAAGCCTGCAGGAGCGGG
  GTCTGCTCCGAGACCAGGTGTTCGGCTGCCAGTTGGAATCACTCTGCCAGCGGGAAGGAGACACGGTGCC
  CAGCTTTTTGCGGCTCTGCATTGCTGCTGTGGATAAAAGAGGTCTAGATGTGGATGGCATTTATCGGGTG
  AGCGGGAACTTGGCAGTGGTCCAGAAGCTTCGCTTTCTGGTGGACAGAGAGCGTGCGGTCACCTCCGATG
  GGAGGTATGTGTTCCCAGAACAGCCAGGACAAGAAGGTCGGTTAGATTTGGACAGTACTGAGTGGGATGA
  CATTCATGTGGTCACCGGAGCCCTGAAGCTTTTTCTCCGGGAGCTGCCCCAGCCTCTGGTGCCACCACTG
  CTGCTGCCCCATTTCCGTGCTGCCCTTGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211794 representing NM_001080157
  Red=Cloning site Green=Tags(s)
  
  MLSSRWWPSSWGILGLGPRSPPRGSQLCALYAFTYTGADGQQVSLAEGDRFLLLRKTNSDWWLARRLEAP
  STSRPIFVPAAYMIEESIPSQSPTTVIPGQLLWTPGPKLFHGSLEELSQALPSRAQASSEQPPPLPRKMC
  RSVSTDNLSPSLLKPFQEGPSGRSLSQEDLPSEASASTAGPQPLMSEPPVYCNLVDLRRCPRSPPPGPAC
  PLLQRLDAWEQHLDPNSGRCFYINSLTGCKSWKPPRRSRSETNPGSMEGTQTLKRNNDVLQPQAKGFRSD
  TGTPEPLDPQGSLSLSQRTSQLDPPALQAPRPLPQLLDDPHEVEKSGLLNMTKIAQGGRKLRKNWGPSWV
  VLTGNSLVFYREPPPTAPSSGWGPAGSRPESSVDLRGAALAHGRHLSSRRNVLHIRTIPGHEFLLQSDHE
  TELRAWHRALRTVIERLDRENPLELRLSGSGPAELSAGEDEEEESELVSKPLLRLSSRRSSIRGPEGTEQ
  NRVRNKLKRLIAKRPPLQSLQERGLLRDQVFGCQLESLCQREGDTVPSFLRLCIAAVDKRGLDVDGIYRV
  SGNLAVVQKLRFLVDRERAVTSDGRYVFPEQPGQEGRLDLDSTEWDDIHVVTGALKLFLRELPQPLVPPL
  LLPHFRAALG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080157
• ORF Size: 1920 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080157.2
• RefSeq Size: 2604 bp
• RefSeq ORF: 1923 bp
• Locus ID: 64333
• UniProt ID: Q9BRR9
• Cytogenetics: 12q13.3
• MW: 70.7 kDa
• Gene Summary: This gene encodes a member of the Rho-GAP family of GTPase activating proteins. The protein has substantial GAP activity towards several Rho-family GTPases in vitro, converting them to an inactive GDP-bound state. It is implicated in regulating adhesion of hematopoietic cells to the extracellular matrix. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]