ENGASE (NM_001042573) Human Tagged ORF Clone

CAT#: RC211494

ENGASE (Myc-DDK-tagged)-Human endo-beta-N-acetylglucosaminidase (ENGASE)

Product Images

Circular map for RC211494

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  Western blot validation of overexpression lysate (Cat# LY420986) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211494 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ENGASE protein (Cat# TP311494). The protein was produced from HEK293T cells transfected with ENGASE cDNA clone (Cat# RC211494) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ENGASE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211494 representing NM_001042573
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCGCGGCGGTGACGGTCACCCGGTCGGCTACACGGCGGCGGCGGCGGCAGCTGCAGGGGCTGG
  CGGCCCCGGAGGCGGGGACGCAGGAGGAGCAGGAGGATCAGGAGCCGCGGCCGCGGCGGCGGCGGCCGGG
  AAGGAGCATCAAAGATGAAGAAGAAGAGACAGTCTTTCGAGAGGTGGTCAGTTTTTCCCCGGACCCCCTG
  CCAGTTAGATATTATGACAAGGACACCACCAAACCAATCAGCTTTTACTTGTCTTCGCTGGAGGAGCTCT
  TGGCGTGGAAGCCCCGCTTGGAGGATGGCTTTAATGTGGCCCTGGAGCCCCTGGCGTGTCGCCAGCCCCC
  TCTGAGCAGCCAGAGGCCCCGGACTTTGTTGTGTCATGACATGATGGGCGGGTACCTGGATGACAGGTTC
  ATTCAGGGCTCGGTGGTGCAGACTCCCTATGCTTTCTACCACTGGCAGTGCATCGACGTCTTTGTGTACT
  TCAGCCACCACACCGTCACCATTCCCCCAGTGGGCTGGACCAACACTGCCCACAGGCATGGGGTCTGCGT
  GCTGGGGACTTTCATCACGGAGTGGAATGAAGGGGGAAGGCTCTGTGAAGCCTTCCTGGCCGGGGATGAG
  CGCTCGTACCAGGCAGTGGCTGACCGGCTGGTCCAGATCACTCAGTTTTTTCGTTTTGATGGCTGGCTGA
  TCAACATCGAGAACTCGCTGAGTCTGGCCGCTGTGGGGAACATGCCTCCTTTCCTGCGGTACCTCACCAC
  ACAGCTGCACCGGCAGGTCCCAGGGGGCCTGGTGCTCTGGTATGACAGCGTGGTGCAAAGTGGGCAGCTC
  AAATGGCAAGACGAACTCAACCAGCACAACAGGGTCTTCTTTGATTCCTGCGACGGCTTCTTCACTAACT
  ATAACTGGCGGGAGGAGCACTTGGAGCGGATGCTGGGGCAGGCTGGGGAGCGCCGGGCTGATGTGTACGT
  GGGCGTGGATGTGTTTGCTCGAGGGAACGTGGTCGGAGGCCGATTCGACACAGACAAGTCGTTGGAGCTG
  ATCCGAAAGCATGGCTTCTCCGTGGCTTTGTTTGCCCCCGGCTGGGTGTATGAGTGTCTGGAGAAGAAGG
  ATTTCTTCCAGAACCAGGACAAGTTCTGGGGCCGACTGGAGCGTTATCTGCCCACACATAGCATCTGCTC
  CTTGCCTTTCGTCACGTCCTTCTGCCTGGGCATGGGTGCACGGAGGGTCTGCTATGGCCAGGAAGAGGCG
  GTAGGGCCCTGGTACCACCTGAGCGCCCAGGAGATCCAGCCCTTGTTTGGAGAACACAGGCTGGGAGGGG
  ATGGCCGGGGCTGGGTGAGGACGCACTGCTGCCTGGAGGATGCCTGGCACGGAGGCAGCTCCCTGCTCGT
  CCGGGGTGTGATCCCACCGGAGGTTGGAAATGTGGCTGTGAGGTTATTTTCCCTGCAGGCCCCAGTGCCA
  CCCAAGATTTACCTGTCCATGGTGTATAAGCTTGAGGGGCCCACGGACGTCACAGTTGCTTTGGAGCTGA
  CCACAGGGGATGCCGGCAGCTGCCACATCGGTGGCATCTCAGTGTTGAACGCAGAAACAAGCTCAAGACA
  CAGCCTCCGACCCCTCCGGGTGCCCCCCACCAAGCTGGCCAGATGGGTGGGCCGCTGCGGCCGGCAGCTG
  AGTGGGGGCTGGGTCCAGCACTGCTACGAGGTGAGCCTGCGTGGGTGCCTGCTGCTAGACCTCCTCGTTT
  GCTTCTCACGGCCGCCGGGTAGTCGGGAGGAGGAGAGCTTCACCTGTCGGCTTGGAGAGATCCAGGTGGT
  GGACGCTGCCAGCCTGCTGGCCCCTCTGCCCCAGGTGCAGGCCGTCACCATCTCTCACATCCGCTGGCAG
  CCATCCGCCTCTGAGCGGGAGGGGCCCCCTGCTCTGCTCCAGCTCAGCTGCACCCTGCACTGGTCCTTCC
  TCCTCTCACAAGTCCGTTGCTTCCGAATCCACTGCTGGGGAGGGATGAGTGATGACTCTCCGGGCAGGGA
  GCTGCCGAGGCCAGAGATGCCCATGTTCCTGGGGTTGGCTTTTGCCACCCAGTACCGGATAGTGGACCTG
  CTGGTGGAAGCCGCCGGGCCCGGCCAGGATCGTCGCATGGAATTTCTGGTGGAGCCTGTCCCCAAGGAAG
  GGTTCCGGGTACCTCAGGCCGAGTGGGGCAGGGCAGTTCTGCTTTATTCAGCCCCTGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211494 representing NM_001042573
  Red=Cloning site Green=Tags(s)
  
  MEAAAVTVTRSATRRRRRQLQGLAAPEAGTQEEQEDQEPRPRRRRPGRSIKDEEEETVFREVVSFSPDPL
  PVRYYDKDTTKPISFYLSSLEELLAWKPRLEDGFNVALEPLACRQPPLSSQRPRTLLCHDMMGGYLDDRF
  IQGSVVQTPYAFYHWQCIDVFVYFSHHTVTIPPVGWTNTAHRHGVCVLGTFITEWNEGGRLCEAFLAGDE
  RSYQAVADRLVQITQFFRFDGWLINIENSLSLAAVGNMPPFLRYLTTQLHRQVPGGLVLWYDSVVQSGQL
  KWQDELNQHNRVFFDSCDGFFTNYNWREEHLERMLGQAGERRADVYVGVDVFARGNVVGGRFDTDKSLEL
  IRKHGFSVALFAPGWVYECLEKKDFFQNQDKFWGRLERYLPTHSICSLPFVTSFCLGMGARRVCYGQEEA
  VGPWYHLSAQEIQPLFGEHRLGGDGRGWVRTHCCLEDAWHGGSSLLVRGVIPPEVGNVAVRLFSLQAPVP
  PKIYLSMVYKLEGPTDVTVALELTTGDAGSCHIGGISVLNAETSSRHSLRPLRVPPTKLARWVGRCGRQL
  SGGWVQHCYEVSLRGCLLLDLLVCFSRPPGSREEESFTCRLGEIQVVDAASLLAPLPQVQAVTISHIRWQ
  PSASEREGPPALLQLSCTLHWSFLLSQVRCFRIHCWGGMSDDSPGRELPRPEMPMFLGLAFATQYRIVDL
  LVEAAGPGQDRRMEFLVEPVPKEGFRVPQAEWGRAVLLYSAPA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042573
• ORF Size: 2229 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042573.3
• RefSeq Size: 4490 bp
• RefSeq ORF: 2232 bp
• Locus ID: 64772
• UniProt ID: Q8NFI3
• Cytogenetics: 17q25.3
• Protein Pathways: Other glycan degradation
• MW: 83.8 kDa
• Gene Summary: This gene encodes a cytosolic enzyme which catalyzes the hydrolysis of peptides and proteins with mannose modifications to produce free oligosaccharides. [provided by RefSeq, Feb 2012]