ZNF571 (NM_016536) Human Tagged ORF Clone

CAT#: RC211426

ZNF571 (Myc-DDK-tagged)-Human zinc finger protein 571 (ZNF571)

Product Images

Circular map for RC211426

• 

  Western blot validation of overexpression lysate (Cat# LY413921) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211426 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF571
• Synonyms: HSPC059
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211426 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCACTTGTTGGTGACTTTCAGGGATGTGGCCATTGACTTCTCTCAGGAGGAATGGGAATGCCTGG
  ACCCTGCTCAGAGGGACTTGTACAGGGATGTGATGTTGGAGAACTACAGCAACCTGATCTCATTGGACTT
  GGAATCCAGTTGTGTGACCAAAAAGTTATCTCCAGAAAAGGAAATTTATGAAATGGAATCACTCCAGTGG
  GAGAATATGGGGAAACGTATCAACCATCACCTTCAATACAATGGTCTTGGAGACAATATGGAGTGCAAAG
  GCAACTTAGAGGGTCAAGAAGCAAGTCAGGAAGGGCTTTACATGTGTGTCAAAATTACCTGTGAAGAAAA
  GGCCACTGAAAGTCATTCAACCTCTTCTACTTTTCATCGGATAATTCCTACCAAGGAAAAATTGTACAAA
  TGTAAGGAATGCAGACAAGGTTTCAGCTACCTGTCATGCCTTATTCAACATGAGGAAAATCATAATATAG
  AAAAATGCTCTGAAGTTAAGAAACACAGGAATACCTTTAGCAAAAAGCCAAGCTATATTCAACATCAGAG
  AATTCAGACTGGTGAGAAACCTTATGAGTGTATGGAATGTGGAAAGGCCTTTGGTCGTACTTCTGATCTC
  ATTCAACATCAGAAAATTCATACTAATGAAAAACCTTATCAGTGTAACGCATGTGGGAAAGCTTTTATTC
  GTGGTTCACAGCTCACTGAACATCAGAGAGTTCATACAGGAGAGAAACCATATGATTGTAAGAAATGTGG
  AAAAGCCTTTAGTTATTGTTCACAATATACTCTTCATCAGAGAATTCATAGTGGTGAAAAACCCTATGAA
  TGTAAGGATTGTGGGAAGGCCTTTATTCTTGGCTCTCAACTTACTTACCATCAGAGAATTCATAGTGGTG
  AGAAACCTTATGAGTGTAAGGAATGTGGAAAGGCCTTTATTCTTGGTTCACACCTTACATACCATCAGAG
  AGTTCATACTGGTGAAAAGCCTTACATATGTAAAGAATGTGGGAAAGCCTTTTTATGTGCCTCCCAACTG
  AATGAACATCAGAGAATTCATACAGGAGAGAAACCCTATGAATGTAAAGAATGCGGGAAGACCTTTTTTC
  GTGGCTCACAACTTACTTACCACCTGAGAGTTCATTCAGGTGAGAGACCTTATAAATGCAAAGAATGTGG
  GAAAGCCTTTATTTCTAATTCTAATCTTATTCAACATCAAAGAATTCATACCGGAGAGAAGCCCTACAAA
  TGTAAGGAATGTGGAAAGGCCTTTATTTGTGGCAAACAACTTAGTGAACATCAGAGAATTCATACAGGTG
  AGAAACCCTTTGAATGTAAGGAATGTGGAAAGGCCTTTATTCGTGTTGCATATCTTACTCAACATGAGAA
  AATTCATGGTGAGAAACATTATGAATGTAAGGAATGTGGGAAGACCTTTGTACGTGCTACACAACTTACA
  TATCATCAAAGAATTCATACAGGTGAAAAGCCCTACAAATGTAAGGAATGTGACAAGGCCTTTATTTATG
  GCTCACAACTTAGTGAACATCAGAGAATTCACAGAGGTGAAAAACCTTATGAATGTAAACAGTGTGGGAA
  GGCTTTTATTCGTGGCTCACACCTTACTGAACATCTGAGAACTCATACTGGAGAGAAACCCTATGAATGT
  AAGGAATGTGGGAGGGCCTTTAGTCGTGGCTCAGAACATACTCTGCATCAAAGGATCCATACTGGTGAGA
  AACCCTATACATGTGTCCAGTGTGGTAAAGACTTTAGATGTCCTTCACAACTTACTCAACATACAAGGCT
  TCATAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211426 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPHLLVTFRDVAIDFSQEEWECLDPAQRDLYRDVMLENYSNLISLDLESSCVTKKLSPEKEIYEMESLQW
  ENMGKRINHHLQYNGLGDNMECKGNLEGQEASQEGLYMCVKITCEEKATESHSTSSTFHRIIPTKEKLYK
  CKECRQGFSYLSCLIQHEENHNIEKCSEVKKHRNTFSKKPSYIQHQRIQTGEKPYECMECGKAFGRTSDL
  IQHQKIHTNEKPYQCNACGKAFIRGSQLTEHQRVHTGEKPYDCKKCGKAFSYCSQYTLHQRIHSGEKPYE
  CKDCGKAFILGSQLTYHQRIHSGEKPYECKECGKAFILGSHLTYHQRVHTGEKPYICKECGKAFLCASQL
  NEHQRIHTGEKPYECKECGKTFFRGSQLTYHLRVHSGERPYKCKECGKAFISNSNLIQHQRIHTGEKPYK
  CKECGKAFICGKQLSEHQRIHTGEKPFECKECGKAFIRVAYLTQHEKIHGEKHYECKECGKTFVRATQLT
  YHQRIHTGEKPYKCKECDKAFIYGSQLSEHQRIHRGEKPYECKQCGKAFIRGSHLTEHLRTHTGEKPYEC
  KECGRAFSRGSEHTLHQRIHTGEKPYTCVQCGKDFRCPSQLTQHTRLHN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016536
• ORF Size: 1827 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016536.1
• RefSeq Size: 2311 bp
• RefSeq ORF: 1830 bp
• Locus ID: 51276
• UniProt ID: Q7Z3V5
• Cytogenetics: 19q13.12
• Domains: KRAB, zf-C2H2
• MW: 70.8 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]