Glycogen synthase 2 (GYS2) (NM_021957) Human Tagged ORF Clone

CAT#: RC211375

GYS2 (Myc-DDK-tagged)-Human glycogen synthase 2 (liver) (GYS2)

Product Images

Circular map for RC211375

• 

  Western blot validation of overexpression lysate (Cat# LY411861) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211375 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GYS2 protein (Cat# TP311375). The protein was produced from HEK293T cells transfected with GYS2 cDNA clone (Cat# RC211375) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Glycogen synthase 2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211375 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCGAGGCCGATCCCTCTCTGTAACATCCCTGGGTGGGCTTCCCCAGTGGGAAGTCGAAGAACTTC
  CTGTGGAGGAGTTACTGCTCTTTGAAGTTGCTTGGGAAGTGACCAATAAAGTTGGAGGCATCTATACTGT
  GATTCAGACAAAGGCCAAAACAACAGCAGATGAATGGGGAGAGAACTATTTTCTGATAGGTCCATATTTT
  GAGCATAATATGAAGACTCAGGTGGAACAGTGTGAACCTGTAAATGATGCTGTCAGAAGAGCAGTGGACG
  CAATGAATAAGCATGGCTGCCAGGTGCATTTTGGAAGATGGCTGATAGAAGGAAGTCCTTATGTGGTACT
  TTTTGACATAGGCTATTCAGCTTGGAATCTGGACAGGTGGAAGGGTGACCTCTGGGAAGCATGCAGTGTC
  GGCATTCCTTATCATGACCGAGAAGCCAATGATATGCTGATATTTGGATCTTTAACTGCCTGGTTCTTAA
  AAGAGGTGACAGATCATGCAGATGGTAAATATGTCGTTGCCCAATTCCATGAATGGCAGGCTGGAATTGG
  ACTGATCCTTTCTCGAGCCAGGAAACTTCCTATTGCCACAATATTTACAACCCACGCTACACTACTTGGG
  AGGTATCTCTGTGCAGCAAATATTGATTTCTACAACCATCTTGATAAGTTTAACATTGACAAAGAGGCTG
  GGGAAAGGCAGATTTACCACCGGTACTGCATGGAGCGAGCTTCCGTTCATTGCGCTCACGTGTTCACCAC
  GGTTTCTGAAATAACAGCAATAGAAGCTGAACATATGCTGAAGAGAAAGCCTGATGTAGTTACTCCAAAC
  GGCTTGAATGTTAAGAAATTTTCAGCAGTGCATGAGTTTCAAAATCTACATGCCATGTACAAGGCCAGAA
  TCCAAGATTTTGTTCGAGGTCATTTCTATGGTCATCTCGACTTTGATCTTGAAAAGACTTTGTTCCTTTT
  CATTGCTGGGAGGTATGAGTTTTCAAACAAAGGAGCTGACATCTTCCTAGAATCCTTATCCAGGCTAAAT
  TTCCTGCTGAGGATGCATAAAAGTGACATCACAGTGGTGGTGTTTTTCATTATGCCTGCCAAGACAAATA
  ATTTCAACGTGGAAACCCTGAAAGGACAAGCAGTGCGAAAACAGCTGTGGGATGTTGCACATTCTGTGAA
  GGAAAAGTTTGGAAAAAAACTCTATGATGCATTATTAAGAGGAGAAATTCCTGACCTGAACGATATTTTA
  GATCGAGATGATCTAACAATTATGAAAAGAGCCATCTTTTCAACTCAGCGACAGTCATTGCCCCCAGTGA
  CCACGCACAACATGATTGATGACTCCACCGACCCCATCCTCAGCACCATTAGACGGATTGGACTTTTCAA
  CAACCGCACAGATAGAGTCAAGGTGATTTTGCACCCAGAGTTTCTATCCTCCACCAGTCCCTTACTACCC
  ATGGACTATGAAGAGTTTGTTAGAGGTTGTCATCTTGGAGTATTTCCATCATACTATGAACCCTGGGGTT
  ATACTCCAGCTGAATGCACTGTGATGGGTATCCCCAGTGTGACCACGAATCTCTCCGGGTTTGGCTGTTT
  CATGCAGGAGCACGTGGCTGATCCTACTGCTTACGGTATTTACATCGTTGACAGGCGGTTCCGTTCTCCA
  GATGATTCTTGCAATCAGCTGACTAAGTTTCTCTATGGATTTTGCAAACAGTCACGCCGCCAAAGGATTA
  TCCAGAGGAACAGAACTGAGAGGCTCTCAGATCTTCTGGATTGGAGATACTTAGGCAGATATTACCAGCA
  TGCCAGACACCTGACATTAAGCAGAGCTTTTCCAGATAAATTCCATGTGGAACTAACATCACCACCAACG
  ACAGAAGGATTTAAATATCCCAGGCCTTCCTCAGTACCACCTTCTCCTTCAGGGTCTCAGGCCTCCAGTC
  CTCAGAGCAGTGATGTGGAAGATGAAGTGGAGGATGAGAGATACGATGAGGAAGAGGAGGCTGAAAGGGA
  TCGGTTAAATATCAAGTCACCATTTTCACTGAGCCACGTTCCTCATGGGAAGAAAAAGCTGCATGGTGAA
  TATAAGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211375 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLRGRSLSVTSLGGLPQWEVEELPVEELLLFEVAWEVTNKVGGIYTVIQTKAKTTADEWGENYFLIGPYF
  EHNMKTQVEQCEPVNDAVRRAVDAMNKHGCQVHFGRWLIEGSPYVVLFDIGYSAWNLDRWKGDLWEACSV
  GIPYHDREANDMLIFGSLTAWFLKEVTDHADGKYVVAQFHEWQAGIGLILSRARKLPIATIFTTHATLLG
  RYLCAANIDFYNHLDKFNIDKEAGERQIYHRYCMERASVHCAHVFTTVSEITAIEAEHMLKRKPDVVTPN
  GLNVKKFSAVHEFQNLHAMYKARIQDFVRGHFYGHLDFDLEKTLFLFIAGRYEFSNKGADIFLESLSRLN
  FLLRMHKSDITVVVFFIMPAKTNNFNVETLKGQAVRKQLWDVAHSVKEKFGKKLYDALLRGEIPDLNDIL
  DRDDLTIMKRAIFSTQRQSLPPVTTHNMIDDSTDPILSTIRRIGLFNNRTDRVKVILHPEFLSSTSPLLP
  MDYEEFVRGCHLGVFPSYYEPWGYTPAECTVMGIPSVTTNLSGFGCFMQEHVADPTAYGIYIVDRRFRSP
  DDSCNQLTKFLYGFCKQSRRQRIIQRNRTERLSDLLDWRYLGRYYQHARHLTLSRAFPDKFHVELTSPPT
  TEGFKYPRPSSVPPSPSGSQASSPQSSDVEDEVEDERYDEEEEAERDRLNIKSPFSLSHVPHGKKKLHGE
  YKN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021957
• ORF Size: 2109 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021957.2, NP_068776.1
• RefSeq Size: 3132 bp
• RefSeq ORF: 2112 bp
• Locus ID: 2998
• UniProt ID: P54840
• Cytogenetics: 12p12.1
• Protein Pathways: Insulin signaling pathway, Starch and sucrose metabolism
• MW: 81 kDa
• Gene Summary: The protein encoded by this gene, liver glycogen synthase, catalyzes the rate-limiting step in the synthesis of glycogen - the transfer of a glucose molecule from UDP-glucose to a terminal branch of the glycogen molecule. Mutations in this gene cause glycogen storage disease type 0 (GSD-0) - a rare type of early childhood fasting hypoglycemia with decreased liver glycogen content. [provided by RefSeq, Dec 2009]